Last-Century Forest Dynamics in a Highland Pyrenean National Park and Implications for Conservation.

Ecological records from before and after the creation of natural parks are valuable for informing conservation and management but are often unavailable. High-resolution paleoecological studies may bridge the gap and provide the required information. This paper presents a 20th-century subdecadal reconstruction of vegetation and landscape dynamics in a national park of the Pyrenean highlands. The park lands had traditionally been used for cultivation, extensive grazing, forest exploitation, and hydroelectricity generation following the damming of numerous glacial lakes. A significant finding is that forests have dominated the landscape, with negligible changes in composition, and only experienced fluctuations in forest cover, influenced by both climatic and anthropogenic factors. The creation of the park (1955) and the initial restrictions on forest exploitation did not significantly affect vegetation cover or composition. Major forest expansion did not occur until several decades later, 1980, when the park was enlarged and forest exploitation was further restricted. This expansion peaked in the 1990s, coinciding with a warming trend and a decrease in fire incidence, before declining due to warmer and drier climates. This decline was coeval with the ongoing global forest dieback and may be exacerbated by the predicted global warming in this century, which could also increase fire incidence due to dead-wood accumulation. Currently, the main threats are global warming/drying, fire, and tourism intensification. Similar high-resolution paleoecological records in protected areas are globally scarce and would be capable in providing the long-term ecological scope required to properly understand forest dynamics and optimize conservation measures.

Diatom responses to warming, heavy rains and human impact in a Mediterranean lake since the preindustrial period.

In the Mediterranean region, annual mean air temperature will continue to increase during the 21st century, while seasonal precipitation is expected to decrease and extreme events to be more frequent. Human-induced climate change will severely impact aquatic ecosystems. A subdecadal stratigraphic diatom record of Lake Montcortès (central Pyrenees) was investigated, focusing on the potential responses of diatoms to anthropogenic warming and catchment alteration. The study includes the end of the Little Ice Age (LIA), the transition to the industrial and postindustrial eras, and the recent global warming and its current acceleration. Sediment samples were treated and diatoms taxonomically identified. Relationships between diatom taxa abundances and climatic (temperature and precipitation) and environmental (land use, soil erosion, and eutrophication) variables were investigated using multivariate statistical methods. The results indicate that, from ca. 1716 to 1971 CE, the diatom community was dominated by Cyclotella cyclopuncta and showed small perturbations, despite the pressure of important stressors such as strong cooling episodes, droughts and an intense use of the lake for hemp retting during the 18th and 19th centuries. However, during the 20th century, other centric species gained relevance, and from the 1970s on, Cyclotella ocellata competed with C. cyclopuncta for dominance. These changes coincided with pulse-like disturbances in the form of extreme rainfall events along with the gradual 20th century increase in global temperature. These perturbations affected the planktonic diatom community and led to instability dynamics. The benthic diatom community did not reflect any comparable shifts under the effect of the same climatic and environmental variables. Because heavy rainfall episodes are likely to intensify with current climate change in the Mediterranean region, their importance as stressors of planktonic primary producers should be taken into account as potential disrupters of biogeochemical cycles and trophic networks of lakes and ponds.

Regional precipitation trends since 1500 CE reconstructed from calcite sublayers of a varved Mediterranean lake record (Central Pyrenees).

The Mediterranean region is expected to be highly impacted by global warming, although the uncertainty of future scenarios, particularly about precipitation patterns remains quite large. To better predict shifts in its current climate system and to test models, more regional climate records are needed spanning longer than the instrumental period. Here we provide a high-resolution reconstruction of autumn precipitation for the Central Pyrenees since 1500 CE based on annual calcite sublayer widths from Montcortès Lake (Central southern Pyrenees) varved sediments. The 500-yr calcite data series was detrended and calibrated with instrumental climate records by applying correlations and cross-correlations to regional precipitation anomalies. Highest relationships were obtained between a composite calcite series and autumn precipitation anomalies for the complete calibration period (1900-2002) and for the two halves of the full period. Applied statistical tests were significant, evidencing that the climatic signal could be reconstructed. The reconstructed precipitation anomalies show interdecadal shifts, and rainfall decrease within the coldest period of the LIA and during the second half of the 20th century, probably associated to current Global Warming. Neither increasing nor decreasing linear trends or periods of extreme precipitation events were identified. Our results are coherent with other palaeohydrological reconstructions for northern Iberian Peninsula. Correlations between the predicted autumn precipitation and the main teleconnections -NAO, ENSO and WEMO- were weak, although a potential relationship with the Atlantic Multidecadal Oscillation (AMO) pattern is suggested. The obtained reconstruction provides the first estimations of regional autumn precipitation shifts in the Central Pyrenees and is one of the few reconstructions that cover annual-to-century scale climate variability of precipitation in the Mediterranean region from the end of the Litte Ice Age (LIA) to the current period of Global Warming.

DNA metabarcoding reveals differences in distribution patterns and ecological preferences among genetic variants within some key freshwater diatom species.

Our study evaluates differences in the distribution and ecology of genetic variants within several ecologically important diatom species that are also key for Water Framework Directive monitoring of European rivers: Fistulifera saprophila (FSAP), Achnanthidium minutissimum (ADMI), Nitzschia inconspicua (NINC) and Nitzschia soratensis (NSTS). We used DADA2 to infer amplicon sequence variants (ASVs) of a short rbcL barcode in 531 environmental samples from biomonitoring campaigns in Catalonia and France. ASVs within each species showed different distribution patterns. Threshold Indicator Taxa ANalysis revealed three ecological groupings of ASVs in both ADMI and FSAP. Two of these in each species were separated by opposite responses to calcium and conductivity. Boosted regression trees additionally showed that both variables greatly influenced the occurrence of these groupings. A third grouping in FSAP was characterized by a negative response to total organic carbon and hence was better represented in waters with higher ecological status than the other FSAP ASVs, contrasting with what is generally assumed for the species. In the two Nitzschia species, our analyses confirmed earlier studies: NINC preferred higher levels of calcium and conductivity. Our findings suggest that the broad ecological tolerance of some diatom species results from overlapping preferences among genetic variants, which individually show much more restricted preferences and distributions. This work shows the importance of studying the ecological preferences of genetic variants within species complexes, now possible with DNA metabarcoding. The results will help reveal and understand biogeographical distributions and facilitate the development of more accurate biological indexes for biomonitoring programmes.

Historical shifts in oxygenation regime as recorded in the laminated sediments of lake Montcortès (Central Pyrenees) support hypoxia as a continental-scale phenomenon.

Recent expansion of anoxia has become a global issue and there is potential for worsening under global warming. At the same time, obtaining proper long-term instrumental oxygen records is difficult, thus reducing the possibility of recording long-term changes in oxygen shifts that can be related with climate or human influence. Varved lake sediments provide the better time frame to study this phenomenon at high resolution. We tracked the oxic/anoxic shifts of the varved Lake Montcortès since 1500CE, and tried to recognise anthropogenic and climatic influences combining biological and geochemical proxies. Four main scenarios emerged: 1) years with abrupt sediment inputs (A); 2) years with outstanding mixing and oxygenation of the water column (B); 3) years with strong stratification, anoxia, intense sulfur bacterial activity and increased biomass production (C); 4) years with stratification and anoxia, but relatively less biomass production (D). In line with current limnologic trends, high supra-annual variability in the occurrence of oxygenation events was observed. Interestingly, at least 45.3% of the years were mixing years and, like the meromictic ones, were mostly clustered into groups of consecutive years, thus alternating years of monomixis with years of meromixis. Most years of D belong to the period 1500-1820CE, when human activities were the most intense. Most years of A belonged to the climatic unstable period of 1850-1899CE. Years of B were irregularly distributed but were best represented in the period 1820-1849CE. Most years of C belonged to the 20th century. More than 90% of the years with climatic instrumental records belonged to B and C. Current climate warming seems to be taking control over the oxygenation capacity of the lake, especially since the second half of the 20th century. Our results support recent findings related to hypoxia spreading at the global scale.

Connection between El Niño-Southern Oscillation events and river nitrate concentrations in a Mediterranean river.

The causes of interannual nitrate variability in rivers remain uncertain, but extreme climatic events have been suggested as drivers of large nitrate inputs to rivers. Based on a 24-year data set (1983-2006), we suggest that El Niño-Southern Oscillation (ENSO) can affect nitrate behavior in a seasonal extra-tropical stream, the Llobregat (NE Iberian Peninsula), located thousands of kilometers away from the ENSO oscillating system via atmospheric teleconnections. Two commonly used indices, the Southern Oscillation Index (SOI) and the self-calibrating-Palmer Drought Severity Index (scPDSI) showed highly significant correlations with nitrate concentrations, which recurrently increased during La Niña phases, coinciding with severe droughts.