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1.
J Neurosci ; 28(14): 3631-43, 2008 Apr 02.
Article in English | MEDLINE | ID: mdl-18385322

ABSTRACT

Cyclin-dependent kinase 5 (Cdk5) is predominantly expressed in the nervous system, where it is involved in neuronal migration, synaptic transmission, and survival. The role of Cdk5 in synaptic transmission is mediated by regulating the cellular functions of presynaptic proteins such as synapsin, Munc18, and dynamin 1. Its multifunctional role at the synapse is complex and probably involves other novel substrates. To explore this possibility, we used a yeast two-hybrid screen of a human cDNA library with p35 as bait and isolated human septin 5 (SEPT5), known also as hCDCrel-1, as an interacting clone. Here we report that p35 associates with SEPT5 in GST (glutathione S-transferase)-pull-down and coimmunoprecipitation assays. We confirmed that Cdk5/p35 phosphorylates SEPT5 in vitro and in vivo and identified S327 of SEPT5 as a major phosphorylation site. A serine (S)-to-alanine (A) 327 mutant of SEPT5 bound syntaxin more efficiently than SEPT5 wild type. Additionally, coimmunoprecipitation from synaptic vesicle fractions and Cdk5 wild-type and knock-out lysates showed that phosphorylation of septin 5 by Cdk5/p35 decreases its binding to syntaxin-1. Moreover, mutant nonphosphorylated SEPT5 potentiated regulated exocytosis more than the wild type when each was expressed in PC12 cells. These data suggest that Cdk5 phosphorylation of human septin SEPT5 at S327 plays a role in modulating exocytotic secretion.


Subject(s)
Cell Cycle Proteins/metabolism , Cyclin-Dependent Kinase 5/physiology , Exocytosis/physiology , Neurons/physiology , Animals , Binding Sites/physiology , Brain/cytology , Brain/metabolism , Cells, Cultured , Cricetinae , Cricetulus , Embryo, Mammalian , Growth Hormone/metabolism , Humans , Immunoprecipitation/methods , Mutation/physiology , Nerve Tissue Proteins/metabolism , Neurons/drug effects , Phosphorylation , Protein Binding , Protein Structure, Tertiary/physiology , RNA, Small Interfering/metabolism , Rats , SNARE Proteins/metabolism , Septins , Serine/metabolism , Synaptic Vesicles/metabolism , Syntaxin 1/metabolism , Transfection/methods
2.
Exp Cell Res ; 313(10): 2098-109, 2007 Jun 10.
Article in English | MEDLINE | ID: mdl-17498690

ABSTRACT

Phosphorylation of types III and IV intermediate filaments (IFs) is known to regulate their organization and function. Phosphorylation of the amino-terminal head domain sites on types III and IV IF proteins plays a key role in the assembly/disassembly of IF subunits into 10 nm filaments, and influences the phosphorylation of sites on the carboxyl-terminal tail domain. These phosphorylation events are largely under the control of second messenger-dependent protein kinases and provide the cells a mechanism to reorganize the IFs in response to the changes in second messenger levels. In mitotic cells, Cdk1, Rho kinase, PAK1 and Aurora-B kinase are believed to regulate vimentin and glial fibrillary acidic protein phosphorylation in a spatio-temporal manner. In neurons, the carboxyl-terminal tail domains of the NF-M and NF-H subunits of heteropolymeric neurofilaments (NFs) are highly phosphorylated by proline-directed protein kinases. The phosphorylation of carboxyl-terminal tail domains of NFs has been suspected to play roles in forming cross-bridges between NFs and microtubules, slowing axonal transport and promoting their integration into cytoskeleton lattice and, in doing so, to control axonal caliber and stabilize the axon. The role of IF phosphorylation in disease pathobiology is discussed.


Subject(s)
Intermediate Filament Proteins/chemistry , Intermediate Filament Proteins/metabolism , Intermediate Filaments/metabolism , Protein Kinases/metabolism , Animals , Axons/metabolism , Axons/ultrastructure , Humans , Intermediate Filament Proteins/genetics , Intermediate Filaments/ultrastructure , Neurofilament Proteins/chemistry , Neurofilament Proteins/metabolism , Phosphorylation , Protein Structure, Tertiary/physiology , Protein Subunits/chemistry , Protein Subunits/genetics , Protein Subunits/metabolism , Vimentin/chemistry , Vimentin/genetics , Vimentin/metabolism
3.
J Biol Chem ; 277(1): 528-34, 2002 Jan 04.
Article in English | MEDLINE | ID: mdl-11684694

ABSTRACT

Cyclin-dependent protein kinase 5 (cdk5), a member of the cdk family, is active mainly in postmitotic cells and plays important roles in neuronal development and migration, neurite outgrowth, and synaptic transmission. In this study we investigated the relationship between cdk5 activity and regulation of the mitogen-activated protein (MAP) kinase pathway. We report that cdk5 phosphorylates the MAP kinase kinase-1 (MEK1) in vivo as well as the Ras-activated MEK1 in vitro. The phosphorylation of MEK1 by cdk5 resulted in inhibition of MEK1 catalytic activity and the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. In p35 (cdk5 activator) -/- mice, which lack appreciable cdk5 activity, we observed an increase in the phosphorylation of NF-M subunit of neurofilament proteins that correlated with an up-regulation of MEK1 and ERK1/2 activity. The activity of a constitutively active MEK1 with threonine 286 mutated to alanine (within a TPXK cdk5 phosphorylation motif in the proline-rich domain) was not affected by cdk5 phosphorylation, suggesting that Thr286 might be the cdk5/p35 phosphorylation-dependent regulatory site. These findings support the hypothesis that cdk5 and the MAP kinase pathway cross-talk in the regulation of neuronal functions. Moreover, these data and the recent studies of Harada et al. (Harada, T., Morooka, T., Ogawa, S., and Nishida, E. (2001) Nat. Cell Biol. 3, 453-459) have prompted us to propose a model for feedback down-regulation of the MAP kinase signal cascade by cdk5 inactivation of MEK1.


Subject(s)
MAP Kinase Signaling System , Mitogen-Activated Protein Kinase Kinases/metabolism , Protein Serine-Threonine Kinases/metabolism , Animals , Cerebral Cortex/metabolism , Down-Regulation , MAP Kinase Kinase 1 , Nerve Tissue Proteins/physiology , PC12 Cells , Phosphorylation , Rats , Threonine/metabolism
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