COX2 Effects on endometrial carcinomas progression.

Uterine corpus cancer is one of the most prevalent gynecologic malignancies, among which endometrial cancers (EC) represent about 90 %. Despite the proven predictive value of several immunohistochemical markers, there remains a need to identify new indicators of EC progression and exploit them for therapeutic purposes. Potential candidates with diagnostic and therapeutic efficacy include cyclooxygenases (COXs). We studied 50 EC cases: 30 endometrioid (EEC), 10 serous (SEC), 10 clear-cell endometrial carcinomas (CCEC) and 10 cases of normal endometrial tissues. We investigated the expression of COX2, ER, PR, Ki-67, EGFR, p53, Bcl-2, VEGF, MMP1, CD31, and CD163 immunohistochemically. COX2 levels in EC tissue are elevated compared to the normal endometrium and depend on tumour histological features and differentiation. Elevated COX2 leads to increased tumour cell proliferation, apoptosis inhibition, increased VEGF expression, microvessel density, and M2 macrophage infiltration, and inhibition of PR expression. ER, EGFR, and MMP1 levels are unaffected by COX2, whose levels are independent of patient age and FIGO stage.

Heavy metals effect on breast cancer progression.

BACKGROUND: Breast cancer is the most frequent localization of malignant process in American women and women of European countries. To date it is not possible to control the morbidity growth due to lack of effective ways of primary prevention. Comparing the incidence of breast cancer in developed countries with the countries of Asia and Africa, there is the fact of population predominance lesion in more urbanized countries. This suggests that the environment along with other factors, occupies a significant place in the initiation and progression of breast neoplasia. The impressive rates of industrial development led to the pollution of soil, surface water and, as a consequence, food by heavy metal salts.The purposes of this paper are as follows: the chemical composition determination of neoplastic breast tissue, evaluation of the DNA methylation level, study of prognostic-important receptors expression in the breast cancer cells, establishing linkages between all the derived indicators. METHODS: In our study we used the following methods: studying of the chemical composition of breast cancer tissue by atomic absorption spectrophotometry and energy-dispersion spectrometer; іmmunohistochemical study of ER, PR, HER2/neu, p53, Ki-67, E-cadherin and MGMT receptors; DNA extraction and investigation by oscillating infrared spectroscopy method. RESULTS: The total amount of heavy metals in breast cancer tissue ranged from 51.21 × 10-3 to 84.86 × 10-3 µg/kg. We have got the following results: the growth of heavy metals in neoplastic tissue is accompanied with the increase of HER2/neu, p53, Ki-67, MGMT expression and decrease of ER and PR expression. The increment of pathological DNA methylation is accompanied with the increasing amount of heavy metals in tumor tissue. CONCLUSIONS: Heavy metals through different pathogenetic links stimulate the progression of breast cancer and reduce its sensitivity to treatment. DNA of tumor tissue has a different level of methylation which changes with the amount of heavy metals in cancer cells. This is displayed on the synthesis of prognostically important receptors in neoplastic tissue.

[Computed tomographic evaluation of the healing of experimental defect of a long bone of the skeleton after implantation into its cavity osteoplastic material "Cerabone®"].

Described in the literature properties of osteoplastic material "Cerabone®" have been obtained from studies in the maxilla and cancellous bone. The lack of data on the impact of «Cerabone®¼ on the dynamics of the healing of defects of compact substance of the long bones of the skeleton dictates the need for such studies. Implantation of osteoplastic material «Cerabone ®¼ was performed into the defect of rat femoral shaft followed by computer-tomographic analysis of its healing. Starting from the 30th day until the end of the experiment (120th day) there were found faint signs of rarefaction in the distal part adjacent to the defect of parent bone, with no signs of bone resorption in its proximal part. There was identified an extremely high density of the implantation site «Cerabone®¼, no visible radiographic evidence of resorption of osteoplastic material and ensuring by the latter the stability of volume of the defect in cortical bone at all stages of the experiment.

STRUCTURAL CHANGES IN SUBMANDIBULAR SALIVARY GLAND, CAUSED BY HEAVY METAL SALTS, AND THEIR CORRECTION WITH DIALIPON (EXPERIMENTAL STUDY).

Setting of possibilities of structural changes correction in submandibular salivary gland with a Dialipone drug, caused by adverse effect of heavy metals salts on an organisms of different age group rats. Structural changes and their correction were studied on the histological preparations basis painted by hematoxylin-eozin, Van-Gizon, PAS reaction, investigated and photographed by means of the digital image display system "SEO Scan". In comparison to the results of submandibular salivary gland tissue histological research of animals without any correction of microelementosis, animals that took Dialipone are marked with a better glandular epithelium saving, less widespreading of dystrophic and dyscirculatory changes, sclerotization processes in stroma. When comparing of Dialipone restoration possibilities, it was set, that different age group animals show different application efficiency of the corrector. The rats of young and mature age have more complete renewal of structural changes. The rats of advanced age show worse reparative capabilities, less complete and effective renewal of structural changes.

[Chemical elements in the blood, bronchial lavage fluid and lung tissue of patients with pulmonary tuberculosis and lung cancer]. / Soderzhanie khimicheskikh elementov v krovi, lavazhnoi zhidkosti bronkhov i legochnoi tkani u bol'nykh tuberkulezom i rakom legkogo.

The examined groups included 58 patients with cancer, 61 subjects with pulmonary tuberculosis and 50 healthy persons as controls. The content of trace elements in blood, bronchial lavage fluid from the most affected portions of the lungs, and in pathologic and healthy lung tissues was determined by a C-115 atomic absorption spectrophotometer. The examination findings demonstrated that the content of blood serum ferric zinc and magnesium, and erythrocyte zinc, manganese, potassium and calcium was higher in patients with pulmonary tuberculosis than in those with lung cancer. At the same time the content of magnesium and calcium in bronchial lavage fluid was higher in persons with a malignant process than in those with pulmonary tuberculosis. As far as differential diagnosis of pulmonary tuberculosis and lung cancer is concerned, it is advisable to measure the levels of erythrocyte trace elements since the latter most precisely reflects their content in the lung pathologic tissue.

[Comparative morphometric analysis of the reaction of the rat bone to dehydration and burn trauma]. / Sravnite'lnyi morfometricheskii analiz reaktsii kostei krys na degidratatsiiu i ozhogovuiu travmu.

The epiphyseal cartilage and diaphysis of the tibia have been studied at dehydration and burn trauma. Dehydration produces certain disturbances in growth and formation of the bone, and burn trauma--development of dystrophic changes.