ABSTRACT
Existing modelling tools, developed to aid the design of efficient molecular wires and to better understand their charge-transport behaviour and mechanism, have limitations in accuracy and computational cost. Further research is required to develop faster and more precise methods that can yield information on how charge transport properties are impacted by changes in the chemical structure of a molecular wire. In this study, we report a clear semilogarithmic correlation between charge transport efficiency and nuclear magnetic resonance chemical shifts in multiple series of molecular wires, also accounting for the presence of chemical substituents. The NMR data was used to inform a simple tight-binding model that accurately captures the experimental single-molecule conductance values, especially useful in this case as more sophisticated density functional theory calculations fail due to inherent limitations. Our study demonstrates the potential of NMR spectroscopy as a valuable tool for characterising, rationalising, and gaining additional insights on the charge transport properties of single-molecule junctions.
ABSTRACT
The characteristic ring with ring appearance in patients with dermatophytosis should raise the suspicion of application of topical corticosteroids (alone or in combination with topical antifungals). Such patients be counselled about the harmful effects applying such inappropriate combinaiton.
Subject(s)
Adrenal Cortex Hormones/adverse effects , Antifungal Agents/adverse effects , Self Medication/adverse effects , Tinea/drug therapy , Tinea/pathology , Administration, Topical , Adolescent , Adrenal Cortex Hormones/therapeutic use , Adult , Aged , Antifungal Agents/therapeutic use , Child , Child, Preschool , Drug Therapy, Combination , Female , Humans , India/epidemiology , Male , Middle Aged , Social Class , Tinea/epidemiology , Young AdultABSTRACT
AIMS: The emergence of multidrug resistant strains of Mycobacterium tuberculosis has made tuberculosis more difficult to manage clinically. With the aim of obtaining new and effective anti-mycobacterial agent(s), this study investigated the anti-mycobacterial activity of several imidazole and piperidine derivatives. METHODS AND RESULTS: Towards obtaining new anti-mycobacterial agents, Mycobacterium smegmatis cells were treated with different compounds for their growth inhibitory activity. Among these, benzyl 1H-imidazole-1-carbodithioate and allyl piperidine-1-carbodiothioate exhibited better inhibition than the others. Thereafter, anti-biofilm property of these two was examined by treating M. smegmatis with these agents before and after the formation of biofilm. The result showed that both the compounds at their sublethal dose inhibited the formation of biofilm as well as dispersed preformed biofilm. Consistently, they augmented the activity of isoniazid or rifampicin against biofilm-encapsulated cells. MTT assay was performed to examine the toxic effects of this combinatorial therapy on different cell lines. Results exhibited a low cytotoxicity for this combinatorial treatment. The activity of these two was also verified against dormant mycobacterial cells and was found to be effective. CONCLUSION: The present study identified two compounds that exhibited anti-mycobacterial activities against both planktonic and dormant cells. These two also exhibited anti-biofilm activity at their sublethal dose and augmented the activity of isoniazid and rifampicin against biofilm encapsulated cells. SIGNIFICANCE AND IMPACT OF THE STUDY: The current study provides two new agents that have the potential to be used in anti-mycobacterial therapy and may help in public health management.
Subject(s)
Anti-Bacterial Agents/pharmacology , Imidazoles/pharmacology , Mycobacterium smegmatis/drug effects , Piperidines/pharmacology , Biofilms/drug effects , Biofilms/growth & development , Humans , Microbial Sensitivity Tests , Rifampin/pharmacologySubject(s)
Drug Eruptions/etiology , Lichen Planus/chemically induced , Rabies Vaccines/adverse effects , Child , Humans , MaleSubject(s)
Arsenic Poisoning/diagnosis , Keratoderma, Palmoplantar/chemically induced , Pigmentation Disorders/chemically induced , Adult , Arsenic Poisoning/pathology , Chronic Disease , Drinking Water/chemistry , Hair/chemistry , Humans , Keratoderma, Palmoplantar/pathology , Male , Nails/chemistry , Pigmentation Disorders/pathologySubject(s)
Erythema Chronicum Migrans/etiology , Lyme Disease/diagnosis , Adult , Anti-Bacterial Agents/therapeutic use , Doxycycline/therapeutic use , Erythema Chronicum Migrans/drug therapy , Erythema Chronicum Migrans/pathology , Humans , Lyme Disease/drug therapy , Lyme Disease/pathology , Male , Treatment OutcomeABSTRACT
In this article, a cleaner, greener, cheaper and environment friendly method for the generation of self assembled silver nanoparticles (Ag NPs) applying a simple irradiation technique using the aqueous extract of the fish scales (which is considered as a waste material) of Labeo rohita is described. Gelatin is considered as the major ingredient responsible for the reduction as well as stabilisation of the self assembled Ag NPs. The size and morphology of the individual Ag NPs can be tuned by controlling the various reaction parameters, such as temperature, concentration, and pH. Studies showed that on increasing concentration and pH Ag NPs size decreases, while on increasing temperature, Ag NPs size increases. The present process does not need any external reducing agent, like sodium borohydride or hydrazine or others and gelatin itself can play a dual role: a 'reducing agent' and 'stabilisation agent' for the formation of gelatin-Ag NPs colloidal dispersion. The synthesized Ag NPs were characterised by Ultraviolet-Visible spectroscopy (UV-Vis), Transmission electron microscopy (TEM) and Selected area electron diffraction (SAED) analyses. The synthesized Ag NPs was used to study the catalytic reduction of various aromatic nitro compounds in aqueous and three different micellar media. The hydrophobic and electrostatic interaction between the micelle and the substrate is responsible for the catalytic activity of the nanoparticles in micelle.
Subject(s)
Gelatin/chemistry , Green Chemistry Technology/methods , Metal Nanoparticles/chemistry , Nitro Compounds/chemistry , Reducing Agents/chemistry , Silver/chemistry , Animals , Biomimetics/methods , Catalysis , Cyprinidae/anatomy & histology , Hydrocarbons, Aromatic/chemistry , Metal Nanoparticles/ultrastructure , Oxidation-ReductionABSTRACT
AIM: Polyethylene succinate (PES) contains hydrolysable ester bonds that make it a potential substitute for polyethylene (PE) and polypropylene (PP). Towards bioremediation of PES, we have already reported that a new strain of Pseudomonas, Pseudomonas sp. AKS2, can efficiently degrade PES and hypothesized that cell surface hydrophobicity plays an important role in this degradation process. In this study, our efforts were targeted towards establishing a correlation between cell surface hydrophobicity and PES degradation. METHODS AND RESULTS: We have manipulated cell surface hydrophobicity of AKS2 by varying concentrations of glucose and ammonium sulphate in the growth medium and subsequently examined the extent of PES degradation. We observed an increase in PES degradation by AKS2 with an increase in cell surface hydrophobicity. The increased surface hydrophobicity caused an enhanced biofilm formation on PES surface that resulted in better polymer degradation. CONCLUSION: The current study establishes a direct correlation between cell surface hydrophobicity of an organism and its potential to degrade a nonpolar polymer like PES. SIGNIFICANCE AND IMPACT OF THE STUDY: Cell surface hydrophobicity manipulation can be used as an important strategy to increase bioremediation of nonpolar polymer like PES.
Subject(s)
Polyethylenes/metabolism , Pseudomonas/metabolism , Succinates/metabolism , Ammonium Sulfate/metabolism , Bacterial Adhesion , Biodegradation, Environmental , Biofilms/growth & development , Cell Membrane/metabolism , Culture Media/chemistry , Glucose/metabolism , Hydrophobic and Hydrophilic Interactions , Polysaccharides, Bacterial/isolation & purification , Polystyrenes/metabolism , Pseudomonas/physiologyABSTRACT
BACKGROUND: Nasal tip reconstruction is a challenging part of septorhinoplasty in post-traumatic patients. Aesthetic appearance of the nose is dependent on adequate tip definition, projection and rotation. Our aim is to establish if caudal septal dislocation (secondary to fracture or subluxation) is a significant factor in causing asymmetry in nostril axis angle (NAA) to effect surgical intervention. In addition, to assess if there is an association between the side of dislocation and the magnitude of NAA. METHODOLOGY: Photographic assessment of NAA was measured in a study group of 26 patients with caudal dislocation of the septum and a control group of 26 patients without caudal septal dislocation. All 52 patients were awaiting a septorhinoplasty procedure due to nasal trauma. RESULTS: Caudal septal dislocation causes a mean difference in NAA of 7.95o compared to 1.38o difference in the control group. We also found there is a greater degree of upward tip rotation on the side of the dislocation resulting in a larger NAA. CONCLUSION: Caudal dislocation of the septum causes a significant asymmetry in NAA, with a greater degree of rotation on the side of the dislocation. We should be aware of this fallacy prior to surgery.
Subject(s)
Nasal Septum/injuries , Nasal Septum/surgery , Nose Deformities, Acquired/surgery , Nose/injuries , Nose/surgery , Rhinoplasty/methods , Case-Control Studies , Female , Humans , Male , Photography , Statistics, NonparametricABSTRACT
Albinism represents a group of genetic disorders with a broad spectrum of hypopigmentary phenotypes dependent on the genetic background of the patients. Oculocutaneous albinism (OCA) patients have little or no pigment in their eyes, skin and hair, whereas ocular albinism (OA) primarily presents the ocular symptoms, and the skin and hair color may vary from near normal to very fair. Mutations in genes directly or indirectly regulating melanin production are responsible for different forms of albinism with overlapping clinical features. In this study, 27 albinistic individuals from 24 families were screened for causal variants by a PCR-sequencing based approach. TYR, OCA2, TYRP1, SLC45A2, SLC24A5, TYRP2 and SILV were selected as candidate genes. We identified 5 TYR and 3 OCA2 mutations, majority in homozygous state, in 8 unrelated patients including a case of autosomal recessive ocular albinism (AROA). A homozygous 4-nucleotide novel insertion in SLC24A5 was detected in a person showing with extreme cutaneous hypopigmentation. A potential causal variant was identified in the TYRP2 gene in a single patient. Haplotype analyses in the patients carrying homozygous mutations in the classical OCA genes suggested founder effect. This is the first report of an Indian AROA patient harboring a mutation in OCA2. Our results also reveal for the first time that mutations in SLC24A5 could contribute to extreme hypopigmentation in humans.
Subject(s)
Albinism/genetics , Albinism/epidemiology , Genes, Recessive , Humans , India/epidemiology , Mutation , Polymerase Chain ReactionSubject(s)
Antineoplastic Agents/adverse effects , Benzamides/adverse effects , Drug Eruptions/etiology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy , Piperazines/adverse effects , Pyrimidines/adverse effects , Adult , Antineoplastic Agents/therapeutic use , Benzamides/therapeutic use , Drug Eruptions/pathology , Exanthema/chemically induced , Exanthema/drug therapy , Hand-Foot Syndrome , Humans , Imatinib Mesylate , Male , Piperazines/therapeutic use , Pyrimidines/therapeutic use , Treatment OutcomeABSTRACT
Cigarette smoke (CS), a major risk factor for developing lung cancer, is known to activate transcriptional activator nuclear factor kappa B (NF-κB). However, the underlying mechanism of this activation remains unclear because of conflicting reports. As NF-κB has a pivotal role in the generation and maintenance of malignancies, efforts were targeted towards understanding its activation mechanism using both ex vivo and in vivo studies. The results show that CS-induced NF-κB activation mechanism is different from that of other pro-inflammatory signals such as lipopolysaccharide (LPS). The NF-κB dimer that translocates to the nucleus upon stimulation with CS is predominantly composed of c-Rel/p50 and this translocation involves degradation of I-κBÉ and not I-κBα. This degradation of I-κBÉ depends on IKKß activity, which preferentially targets I-κBÉ. Consistently, CS-activated form of IKKß was found to be different from that involved in LPS activation as neither Ser177 nor Ser181 of IKKß is crucial for CS-induced NF-κB activation. Thus, unlike other pro-inflammatory stimulations where p65 and I-κBα have a central role, the predominantly active signaling cascade in CS-induced NF-κB activation in the lung epithelial cells comprises of IKKß-I-κBÉ-c-Rel/p50. Thus, this study uncovers a new axis of NF-κB activation wherein I-κBÉ and c-Rel have the central role.