ABSTRACT
This project presents the results obtained from a new strategy based on Virtual Reality techniques, which intends to minimize the operational issues caused in electric power substations due to the lack of spatial and functional information on the traditional operation interfaces. For this purpose, a three-dimensional interactive virtual reality environment was built in a realistic and accurate way regarding an energy supplier substation in Minas Gerais - Brazil and subsequently implanted it in its operation center for tasks related to its functioning. Lastly, tests were applied to operators to obtain results aiming at the contextualized problems.
Subject(s)
Virtual Reality , Brazil , EnvironmentABSTRACT
Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details to perform heterotopic and partial orthotopic surgical models to promote ALS transplantation. After preservation and vessel cannulation steps, the liver grafts were decellularized. In addition, ex vivo blood perfusion tests were performed to obtain a successful anticoagulation treatment prior in vivo transplantation. Then, methods of partial liver resection, combination of hand-suture and cuff techniques to complete end-to-end anastomosis between the scaffold and the recipient animal were performed. These procedures which take 30-60 min and were efficient to allow acellular liver scaffold viability and recellularization of different types of cell post-surgery. In conclusion, our methods are practical and simple promising approach that provides the opportunity to investigate ways to achieve sufficient liver function post-transplantation in vivo.
