Muscle Strength Indexes and Its Association With Cardiometabolic Risk Factors in Adolescents: An Allometric Approach.

Background and aims: Muscle strength (MS) has been associated with better cardiometabolic health prognosis. However, the result for the beneficial relationship seems to be dependent on the influence of body size in determining MS levels. We investigate the association between allometric MS indexes and its association with cardiometabolic risk factors in adolescents. Methods: It was a cross-sectional study comprising 351 adolescents (male: 44.4%; age range 14-19 years) from Southern Brazil. MS was assessed by handgrip strength and three different allometric approaches were adopted: 1) MS index based on theoretical allometric exponent; 2) MS index including body mass and height; 3) MS index including fat-free mass and height. Obesity, high blood pressure, dyslipidemia, glucose imbalance and high-sensitivity C-reactive protein were investigated as individual factors or as combinations (either as combinations of components - presence of two adverse conditions, or number of components present in an individual - 0, 1, 2, 3+ cardiometabolic risk factors). Logistic and multinomial logistic regression analyses adjusted for confounding factors were used. The statistical significance adopted was 5%. Results: MS index based on theoretical allometric exponent was associated with lower likelihood (OR: 0.54; 95% CI: 0.28 - 0.89) for the presence of three or more cardiometabolic risk factors in the same individual. Conclusion: This study suggests that MS index based on the theoretical allometric exponent can be superior to allometric MS indexes that included body mass and height, or fat-free mass and height in representing the presence of high number of cardiometabolic risk factors in adolescents.

Burden of disease due to amphetamines, cannabis, cocaine, and opioid use disorders in South America, 1990-2019: a systematic analysis of the Global Burden of Disease Study 2019.

BACKGROUND: South America's substance use profile, poverty, income inequality, and cocaine-supplier role make it a unique place for substance use research. This study investigated the burden of disease attributable to amphetamine use disorder, cannabis use disorder (CAD), cocaine use disorder, and opioid use disorder (OUD) in South America from 1990 to 2019, on the basis of the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019. METHODS: GBD 2019 estimated the incidence, prevalence, mortality, years of life lost (YLL), years of life lived with disability (YLD), and disability-adjusted life-years (DALYs) due to substance use disorders in each of the 12 South American countries (Argentina, Bolivia, Brazil, Chile, Colombia, Ecuador, Guyana, Paraguay, Peru, Suriname, Uruguay, and Venezuela). Data were modelled using standardised tools (ie, the Cause of Death Ensemble model, spatio-temporal Gaussian process regression, and disease modelling meta-regression) to generate estimates of each quantity of interest by sex, location, and year. The analysis included comparisons by sex and country, and against regional and global estimates. FINDINGS: In 2019, the highest amphetamine use disorder burden per 100 000 population in South America was in Peru (66 DALYs). CAD DALY rates per 100 000 in South America were stable between 1990 and 2019, except in Chile and Colombia, which had the highest rates in 2019 (19 DALYs for Chile and 18 DALYs for Colombia). OUD DALYs per 100 000 increased during the period in Brazil and Peru, which in 2019 had the highest rates in South America (82 DALYs for Brazil and 70 DALYs for Peru). In 2019, Brazil had the highest cocaine use disorder DALYs per 100 000 (45 DALYs), nearly double its rate in 1990. DALY rates were higher in males than females for each substance use disorder, except in Paraguay. The overall burden of substance use disorders was higher in males than in females, mainly because of cocaine use disorder and CAD, whereas for amphetamine use disorder, the difference between sexes was minimal, and for OUD there was no difference. For males and females, the highest rate of substance use disorders DALYs per 100 000 was for OUD except in Argentina (in males, 58 DALYs for cocaine use disorder vs 52 DALYs for OUD) and in Paraguay (in females, 77 for amphetamine use disorder vs 50 for OUD). CAD DALY rates were generally the lowest among the substance use disorders for males and females. Amphetamine use disorder YLD rates were reasonably stable throughout the period and were highest in Peru, Paraguay, and Uruguay (>40 YLD per 100 000). For CAD, YLD rates were stable in all countries except Chile and Colombia. Cocaine use disorder YLD rates per 100 000 for the top four countries (Argentina, Uruguay, Chile, and Brazil) increased from 1990 to 2010 (eg, from 19 to 33 in Brazil), but decreased between 2010 and 2019 (eg, from 36 to 31 in Chile). For OUD, YLD rates showed a slight increase in most countries apart from Brazil, which increased from 52 in 1990 to 80 in 2019 and was top among the countries. Amphetamine use disorder YLL rates per 100 000 were highest in Suriname and Peru during the period, although in Suriname it increased from 2·7 in 2010 to 3·2 in 2019, whereas in Peru it decreased from 2·1 to 1·7. The highest YLL rate for cocaine use disorder was in Brazil, which increased from 3·7 in 1990 to 18·1 in 2019. Between 2000 and 2019, Chile and Uruguay showed the highest OUD YLL rates (11·6 for Chile and 10·9 for Uruguay). A high incidence of CAD was found in Chile, Colombia, Guyana, and Suriname. There were high incidences of amphetamine use disorder in Paraguay, cocaine use disorder in Argentina, and OUD in Ecuador. A decrease in annual prevalence for substance use disorders during the period was observed in Venezuela (amphetamine use disorder, CAD, and OUD), Brazil (CAD and amphetamine use disorder), Colombia (amphetamine use disorder and cocaine use disorder), Peru (amphetamine use disorder and cocaine use disorder), Chile and Suriname (amphetamine use disorder), Uruguay (CAD), and Bolivia (OUD). Overall, the cocaine use disorder burden stabilised then decreased. OUD was less prevalent than other substance use disorders but its burden was the highest. INTERPRETATION: The decrease in the burden of cocaine use disorder probably reflects the success of national standardised treatment programmes. Programmes for amphetamine use disorder, CAD, and OUD management should be improved. We did not find an increase in CAD burden in Uruguay, the country with the highest degree of cannabis decriminalisation in the region. Countries in South America should improve monitoring of substance use disorders, including regular surveys to provide more accurate data on which to base policy decisions. FUNDING: The Bill & Melinda Gates Foundation.

Anthropometric indicators as discriminators of high body fat in children and adolescents with HIV: comparison with reference methods.

BACKGROUND: Body fat assessment is needed in individuals with HIV. The objective was to identify the discriminatory capacity of the abdominal skinfold (ASF) tricipital skinfold (TSF), subscapular fold (SSF), calf skinfold (CSF), body adiposity index (BAI), body mass index, conicity index (IC), mid-upper arm circumference (MUAC), waist circumference (WC), perimeter of neck (PN) and waist-to-height ratio (WHtR) for high body fat in children and adolescents with HIV, compared Dual energy X-ray absorptiometry (DXA) and air displacement plethysmography (ADP). METHODS: Descriptive study, cross - sectional study, with 65 children and adolescents with HIV by vertical transmission. Body fat was measured by DXA and ADP. Measures were measured by international standardization. The diagnostic properties for high body fat were assessed by area under the ROC curve (AUC). RESULTS: For boys, having DXA as a reference for fat, ASF (AUC: 0.920), TSF (AUC: 0.792), SSF (AUC: 0.766), CSF (AUC: 0.866), BAI satisfactory discriminatory capacity. With ADP as the reference method, ASF (AUC: 0.920), TSF (AUC: 0.921), SSF (AUC: 0.766), CSF (AUC: 0.901), BAI (AUC: 0.756) and BMI (AUC: 0.699) presented satisfactory results. For girls, having DXA as a reference for fat, ASF (AUC: 0.838), TSF (AUC: 0.842), SSF (AUC: 0.840), CSF (AUC: 0.887), BAI (AUC: 0.846), and BMI (AUC: 0.859) presented satisfactory discriminatory capacity. Assuming ADP as a reference for fat, ASF (AUC [AUC: 0.799], TSF [AUC: 0.825], SSF [AUC: 0.767], CSF [AUC: 0.897], BAI 0.788), were satisfactory. CONCLUSIONS: The ASF, TSF, SSF, CSF, BAI and BMI anthropometric indicators may be suggested as the most suitable for the detection of high body fat in children and adolescents with HIV.

Muscle Strength Assessed by Handgrip Strength Moderates the Relationship Between Overweight and Obesity With Cardiometabolic Risk Markers Among Adults and Older Adults.

Background and aims: Few population-based studies have studied whether muscle strength (MS) levels influence the relationship between cardiometabolic markers with excess body weight. We investigate the possible moderator role of MS in the relationship between overweight and obesity with cardiometabolic markers. Methods: Cross-sectional analysis using data from two studies in Florianópolis, Brazil (EpiFloripa Adult Cohort Study, n = 862, 39.3 ± 11.4 years; EpiFloripa Aging Cohort Study, n = 1,197, 69.7 ± 7.1 years). MS was assessed by handgrip strength. Body mass index (BMI) was classified as overweight or obesity (BMI 25.0-29.9 or ≥30.0 kg/m2, respectively). Cardiometabolic markers included systolic (SBP) and diastolic blood pressure (DBP), C-reactive protein (CRP), lipids, glucose markers, and carotid intima-media thickness (CIMT). Linear regression models stratified by age groups (adults: <60 years; older adults: ≥60 years) and adjusted for confounders were used. Results: Compared to those with a normal BMI, overweight and obesity were related to higher SBP, DBP, lnCRP, ln triglycerides, fasting glucose (FG), and glicated hemoglobin (HbA1c), and inversely associated with HDL-C among adults and older adults (p value < .05 for all). Additionally, BMI was positively associated with LDL-C and CIMT only among adults (p value < .05). Among adults and older adults, elevated MS attenuated the adverse relationship between excess body weight with FG and HbA1c. Conclusion: Higher MS might help adults and older adults with overweight or obesity reduce their cardiovascular risk by keeping their FG and HbA1c at the same levels of those with a normal BMI.

Clusters of lifestyle behaviors associated with atherosclerosis risk factors in children and adolescents with congenital heart disease: Floripa CHild Study.

Secondary cardiovascular disease is the main cause of mortality in congenital heart disease (CHD) patients. The cardiovascular risk could be widely prevented with adherence to a healthy lifestyle; however, clusters of lifestyle behaviors related to atherosclerosis risk factors in children and adolescents with CHD remain unclear. We aimed to describe the clusters of lifestyle behaviors of children and adolescents with CHD and to evaluate their association with atherosclerosis risk factors. We conducted a cross-sectional study on 227 children and adolescents with CHD (median age:10.02 [IQR:7.08-13.02] years). Dietary intake, physical activity (PA), and sedentary behavior (SB) were evaluated. Clusters of lifestyle behaviors were determined using a two-step cluster analysis. Atherosclerosis risk factors evaluated include body fat mass, central obesity, blood pressure, lipid parameters, glucose, C-reactive protein, and carotid intima-media thickness (cIMT). Multiple logistic regressions were used. The "unhealthy: high SB + low PA" cluster was associated with elevated body fat mass, central obesity, and elevated cIMT. Furthermore, the "unhealthy: low PA + unhealthy eating habits" cluster was associated with elevated body fat mass, central obesity, and elevated glucose. The unhealthier lifestyle behavior clusters were associated with atherosclerosis risk factors in children and adolescents with CHD. Multidisciplinary strategies to promote healthy behaviors are needed to prevent cardiovascular disease in later life.

Bone Density and Bone Geometry in University Athletes From Sports With Different Levels of Impact: Simultaneous Association With Multiple Factors.

ABSTRACT: Moraes, MS, Fernandes, RA, Moreno, YMF, Pelegrini, A, and Silva, DAS. Bone density and bone geometry in university athletes from sports with different levels of impact: simultaneous association with multiple factors. J Strength Cond Res 36(11): 3113-3121, 2022-The aim of this study was to use the bone loading unit (BLU) classification to compare bone density and bone geometry of sports with different levels of impact, simultaneously controlling the multiple factors that interfere with bone metabolism in athletes. Overall, 167 university athletes (92 men) participated in the study. Bone mineral density (BMD), strength index, cross-sectional area (CSA) at the moment of inertia, section modulus (Z), CSA, neck-shaft angle (NSA), and hip axis length (HAL) were measured. Sports were categorized by level of impact, high BLU (higher impact) and moderate/low BLU (lower impact). Covariates were fat mass, lean tissue mass, training volume, time of practice, dietary supplementation, use of oral contraceptives and menstrual status. Multiple linear regression with 5% significance level ( p < 0.05) was used. In men, HAL was higher in sports with high BLU compared with those with moderate/low BLU (ß: -0.21; R2 : 0.30; p = 0.03). In women, NSA was higher in sports with moderate/low BLU compared with those with high BLU (ß: 0.31; R2 : 0.11; p = 0.02). There was no difference between BLU groups for BMD. These results persisted when the simultaneous interference of covariates was controlled. Thus, this study recommends BLU to classify sports and reinforces the importance of monitoring HAL and NSA in athletes in addition to the control of multiple factors that interfere with bone metabolism, because they influence physical performance and bone health during and after athletic life.

Equations based on anthropometric measurements for adipose tissue, body fat, or body density prediction in children and adolescents: a scoping review.

PURPOSE: Assessing the body composition of children and adolescents is important to monitor their health status. Anthropometric measurements are feasible and less-expensive than other techniques for body composition assessment. This study aimed to systematically map anthropometric equations to predict adipose tissue, body fat, or density in children and adolescents, and to analyze methodological aspects of the development of anthropometric equations using skinfolds. METHODS: A scoping review was carried out following the PRISMA-ScR criteria. The search was carried out in eight databases. The methodological structure protocol of this scoping review was retrospectively registered in the Open Science Framework ( https://osf.io/35uhc/ ). RESULTS: We included 78 reports and 593 anthropometric equations. The samples consisted of healthy individuals, people with different diseases or disabilities, and athletes from different sports. Dual-energy X-ray absorptiometry (DXA) was the reference method most commonly used in developing equations. Triceps and subscapular skinfolds were the anthropometric measurements most frequently used as predictors in the equations. Age, stage of sexual maturation, and peak height velocity were used as complementary variables in the equations. CONCLUSION: Our scoping review identified equations proposed for children and adolescents with a great diversity of characteristics. In many of the reports, important methodological aspects were not addressed, a factor that may be associated with equation bias. LEVEL IV: Evidence obtained from multiple time series analysis such as case studies. (NB: dramatic results in uncontrolled trials might also be regarded as this type of evidence).

Comparison of bone mineral density and bone mineral content in university athletes from different sports.

BACKGROUND: Bone parameters are influenced by multiple factors. However, when comparing sports, few studies have considered the simultaneous interference of these factors in bone parameters. The aim of this study is to compare bone mineral density (BMD) and bone mineral content (BMC) between university athletes from different sports, according to sex. METHODS: Cross-sectional study with 71 male and female university athletes practicing indoor soccer (14 male, 20 female), judo (eight male, six female) and volleyball (11 male, 12 female) aged 22.37±3.71 years. Dependent variables were BMD and BMC corrected by height measured by dual energy X-ray absorptiometry. The sport practiced (indoor soccer, judo and volleyball) was the independent variable and covariates were fat mass, lean tissue mass, training volume, practice time and gynecological aspects (in female athletes) such as regular menstruation and use of oral contraceptives. Analysis of covariance was used, with P<0.05. RESULTS: Female volleyball players (mean: 1.23; standard error [SE]: 0.03) presented with higher lumbar BMD values compared to judo athletes (mean: 1.08; SE: 0.05). In addition, lumbar BMC values of indoor soccer athletes (mean: 0.41; SE: 0.01) was higher compared to judo athletes (mean: 0.34; SE: 0.02). In males, no differences were observed among sports. CONCLUSIONS: Sports performance technicians and professionals will be able to broaden the understanding of variations in BMD and BMC lumbar between judo, indoor soccer and volleyball athletes, which allows monitoring these parameters in the sports context and in the athlete's health.

Prediction of body fat in adolescents: validity of the methods relative fat mass, body adiposity index and body fat index.

OBJECTIVE: To verify the validity of anthropometric methods body adiposity index (BAI), relative fat mass (RFM) and body fat index (BFI) to estimate body fat percentage (%BF) in adolescents. METHODS: A cross-sectional study was carried out with 420 Brazilian adolescents aged 15-19 years, stratified by age (< 18 years, n = 356; ≥ 18 years, n = 64) and sex (boys, n = 216; girls, n = 204). The Anthropometric measurements height, body weight, hip circumference and waist circumference were collected to calculate the %BF by BAI, RFM, BFI methods. Subsequently, %BF was measured by dual emission X-ray absorptiometry (DXA), adopted as a reference method. In the statistical analysis of the data, the Pearson correlation test and the paired t test between %BF obtained by the equations and by the DXA were performed. The method validation criterion was that 68% of individuals should be within an acceptable error range of ± 3.5% of BF and Cohen's Kappa index ≥ 0.61. Additionally, the Bland-Altman graphical analysis was performed. RESULTS: All methods showed a high correlation with DXA. For the Kappa index, only the RFM reached the criterion in the total sample (0.67) and in the sample < 18 years (0.68). None of the methods reached the criterion of 68% of the sample within the error range of ± 3.5% of BF. CONCLUSION: The BAI, RFM and BFI equations were not valid for predicting BF in the studied sample according to the criteria adopted regardless of sex or age. LEVEL OF EVIDENCE: Level V, cross-sectional descriptive study.

An integrative analysis uncovers a new, pseudo-cryptic species of Amazonian marmoset (Primates: Callitrichidae: Mico) from the arc of deforestation.

Amazonia has the richest primate fauna in the world. Nonetheless, the diversity and distribution of Amazonian primates remain little known and the scarcity of baseline data challenges their conservation. These challenges are especially acute in the Amazonian arc of deforestation, the 2500 km long southern edge of the Amazonian biome that is rapidly being deforested and converted to agricultural and pastoral landscapes. Amazonian marmosets of the genus Mico are little known endemics of this region and therefore a priority for research and conservation efforts. However, even nascent conservation efforts are hampered by taxonomic uncertainties in this group, such as the existence of a potentially new species from the Juruena-Teles Pires interfluve hidden within the M. emiliae epithet. Here we test if these marmosets belong to a distinct species using new morphological, phylogenomic, and geographic distribution data analysed within an integrative taxonomic framework. We discovered a new, pseudo-cryptic Mico species hidden within the epithet M. emiliae, here described and named after Horacio Schneider, the pioneer of molecular phylogenetics of Neotropical primates. We also clarify the distribution, evolutionary and morphological relationships of four other Mico species, bridging Linnean, Wallacean, and Darwinian shortfalls in the conservation of primates in the Amazonian arc of deforestation.

Individual and Combined Association Between Healthy Lifestyle Habits With Muscle Strength According to Cardiovascular Health Status in Adults and Older Adults.

BACKGROUND: The authors aimed to identify the effect of adherence to healthy lifestyle habits on muscle strength (MS) according to a distinct health status. METHODS: Longitudinal analysis using data from 2 population-based cohorts in Brazil (EpiFloripa adult, n = 862, 38.8 [11.4] y-6 y of follow-up length; EpiFloripa Aging, n = 1197, 69.7 [7.1] y-5 y of follow-up length). MS was assessed by handgrip strength (kgf). Information assessed by questionnaire regarding adequate physical activity levels, regular consumption of fruit and vegetables, low alcohol consumption, and nonsmoking habits were analyzed in the relationship with MS according to the health status. The participants were grouped into 3 health status categories: (1) with cardiovascular disease (CVD); (2) at risk of CVD (abdominal obesity or overweight/obesity, high blood pressure, hyperglycemia, and dyslipidemia); and (3) healthy individuals (without CVD and risk of CVD). RESULTS: Simultaneous adherence of 4 healthy lifestyle habits was directly associated with MS among healthy individuals (ß = 10.0, 95% CI, 2.0-18.0, SE = 4.0), at risk of CVD (ß = 5.5, 95% CI, 0.3-12.6, SE = 3.6), and those with CVD (ß = 11.4, 95% CI, 5.8-16.7, SE = 2.8). CONCLUSIONS: Adopting a healthy lifestyle can contribute to increased MS in adults and older adults, regardless of health status.

Cell integrity indicators in university athletes: comparison among playing positions in indoor football.

BACKGROUND: The aim of this study was to compare cell integrity indicators according to the playing position in university indoor football athletes. METHODS: The sample consisted of 34 university athletes (20 female and 14 male). Dependent variables were cell integrity indicators: total body water (TBW), intracellular water (ICW), extracellular water (ECW), ECW/ICW ratio, body cell mass (BCM), ECW/BCM ratio, phase angle (PhA), resistance (R), Xc (reactance) and impedance (Z), evaluated by the electrical bioimpedance method. Independent variable was the playing position: goalkeeper (a), defender (a), winger (left and right) and pivot collected through questionnaire. Control variables were age, time of sport practice, participation in competitions per year and training load obtained by applying the anamnesis form, and body fat and fat and bone-free mass were obtained through dual emission X-ray absorptiometry (DXA). Covariance analysis (ANCOVA) was used, with post-hoc Tukey's test, to identify difference between groups, with P<0.05. RESULTS: In the adjusted analysis, female athletes in the defense position had BCM values (31.1±2.1) higher than those in the wing position (25.8±1.1, P<0.01). In males, pivots showed higher ICW values (31.47±0.77) when compared to defenders (25.7±0.8, P=0.02). In addition, goalkeepers had higher TBW values (52.7±2.5) compared to wingers (42.3±1.2) (P=0.03). CONCLUSIONS: Cell integrity indicators may vary according to the playing position in indoor football.

Association between muscle strength and risk factors for metabolic syndrome in children and adolescents: a systematic review.

The aim of this systematic review was to identify and summarize evidence for the association between muscle strength (MS) and metabolic syndrome (MetS), and MS and combinations of risk factors for MetS in children and adolescents. Five databases (Medline/PubMed, EBSCO, Scielo, Scopus, and Web of Knowledge) were searched up to November 2019 with complementary reference list searches. Inclusion criteria were studies that investigated the relationship between MS and MetS or MS and combinations of risk factors for MetS in children and adolescents (≤19 years of age). Risk of bias was assessed using standard procedures. From the total of 15,599 articles initially identified, 13 articles were included, representing 11,641 children and adolescents. Higher MS values were associated with lower risk for MetS or combinations of risk factors for MetS (n=11/13 studies). Of the total of included studies, about 23.1% (03/13) were longitudinal and all included studies were classified as having a moderate risk of bias. This review provides preliminary evidence for a beneficial relationship between MS and MetS among children and adolescents. Additionally, although the body of evidence points to the beneficial relationship between higher MS and lower risk for combination of factors for MetS in children and adolescents, this relationship is inconclusive.

Checklist of mammals from Goiás, central Brazil / Lista de Mamíferos de Goiás, Brasil central

Abstract: The state of Goiás, in central Brazil, is covered mainly by the Cerrado domain, with the Alto Paraná Atlantic Forest occupying its central-southern portion. Goiás is one of the 20 Brazilian federative units without a mammal checklist. In this study, we provide the first checklist of mammals from Goiás state. We recorded mammal species based primarily on the analysis of specimens housed in scientific collections as well as on literature with associated voucher material. We listed 191 mammalian species belonging to 125 genera, 31 families and 10 orders, which represents 25.2% of the mammal species occurring in Brazil. The most speciose orders were Chiroptera (90 spp.), followed by Rodentia (43 spp.), Carnivora (19 spp.) and Didelphimorphia (17 spp.). The following orders accounted for a smaller portion of the state diversity: Cetartiodactyla (7 spp.), Cingulata (7 spp.), Primates (4 spp.), Pilosa (2 spp.), Lagomorpha (1 sp.), and Perissodactyla (1 sp.). A total of 28 species (14.7%), mainly represented by medium and large-sized mammals, are nationally threatened while 12 (6.3%) are globally threatened. Our results indicate great portions of the state lacking a proper survey of mammals, especially the northwestern portion. We discuss species richness, distribution and conservation status of the mammals of Goiás state in national and regional scenarios. We highlight the need for mammal inventories based on complementary survey techniques with the collection of vouchers in order to provide karyologic, molecular, morphologic, parasitologic, and ecological data. These informations are the basis for integrative studies that lead to the understanding of current mammalian richness and diversity. Indeed, knowledge on species richness distribution in the state will guide conservation strategies, especially in areas undergoing habitat loss and fragmentation, such as the central-southern portion of Goiás.

Resumo: O estado de Goiás, no Brasil central, é coberto principalmente pelo domínio do Cerrado, com a Mata Atlântica do Alto Paraná ocupando sua porção centro-sul. Goiás é uma das 20 unidades federativas brasileiras que ainda não possui uma lista de espécies de mamíferos. Neste estudo, apresentamos a primeira lista de mamíferos para o estado. Registramos as espécies de mamíferos com base principalmente na análise de espécimes depositados em coleções científicas, bem como na literatura apresentando material testemunho associado. Listamos 191 espécies pertencentes a 125 gêneros, 31 famílias e 10 ordens, as quais representam 25,2% das espécies de mamíferos que ocorrem no Brasil. As ordens mais especiosas foram Chiroptera (90 spp.), seguida pelas ordens Rodentia (43 spp.), Carnivora (19 spp.) e Didelphimorphia (17 spp.), com as demais ordens respondendo por uma porção menor da diversidade: Cetartiodactyla (7 spp.), Cingulata (7 spp.), Primates (4 spp.), Pilosa (2 spp.), Lagomorpha (1 sp.) e Perissodactyla (1 sp.). Um total de 28 espécies (14,7%), principalmente representadas por mamíferos de médio e grande porte, estão ameaçadas nacionalmente e 12 (6,3%) encontram-se globalmente ameaçadas. Nossos resultados indicaram grandes porções do estado ainda não devidamente pesquisadas em relação aos seus mamíferos, com informações escassas e fragmentadas, principalmente no que diz respeito à sua porção noroeste. Discutimos a riqueza de espécies, a distribuição e o estado de conservação dos mamíferos do estado de Goiás nos cenários nacional e regional. Ressaltamos a importância da realização de inventários que utilizem técnicas complementares de amostragem, incluindo a coleta de material testemunho, proporcionando a obtenção de dados cariotípicos, moleculares, morfológicos, parasitológicos e ecológicos. Estas informações são a base de estudos integrativos, os quais aumentam nossa compreensão a respeito da riqueza e diversidade atual dos mamíferos. O conhecimento a respeito da distribuição da riqueza de espécies em Goiás é essencial para embasar estratégias de conservação, tão necessárias em áreas que vem sofrendo com a perda e fragmentação de seus hábitats naturais, como a porção centro-sul do estado.

Testing validity of FitnessGram in two samples of US adolescents (12-15 years).

BACKGROUND: This study examined the validity of the FitnessGram® criterion-reference cut-points for cardiorespiratory fitness (CRF) based on two samples of US adolescents (aged 12-15 years). This study also established the CRF cut-points for metabolically healthy weight status based on a recent national fitness survey for the purposes of cross-validating with pre-existing cut-points including FitnessGram. METHODS: Two cross-sectional data from the 2003-2004 National Health and Nutrition Examination Survey (NHANES) (n = 378) and 2012 NHANES National Youth Fitness Survey (NNYFS) (n = 451) were used. CRF (estimated V ˙ O2max in mL/kg/min) was estimated from a submaximal exercise test. CRF categories based on FitnessGram cut-points, a clustered cardiometabolic risk factors score and weight status were used. A series of Receiver Operating Characteristic (ROC) curve analyses were conducted to identify age- and sex-specific CRF cut-points that were optimal for metabolically healthy weight status. RESULTS: Based on FitnessGram cut-points, having high risk CRF, but not low risk CRF, was associated with high cardiometabolic risk (OR = 3.17, 95% CI = 1.14-8.79) and unhealthy weight status (OR = 5.81, 95% CI = 3.49-9.68). The optimal CRF cut-points for 12-13-year-olds and 14-15-year-olds were 40 and 43 mL/kg/min in males and 39 and 34 mL/kg/min in females, respectively. Compared to meeting new CRF cut-points, not meeting new CRF cut-points was associated with higher odds of showing high cardiometabolic risk (OR = 2.91, 95% CI = 1.47-5.77) and metabolically unhealthy weight status (OR = 4.47, 95% CI = 2.83-7.05). CONCLUSION: FitnessGram CRF cut-point itself has rarely been scrutinized in previous literature. Our findings provide partial support for FitnessGram based on two samples of US adolescents. CRF cut-points established in this study supports international criterion-referenced cut-points as well as FitnessGram cut-points only for males. FitnessGram should be continuously monitored and scrutinized using different samples.

Assessing body composition in rugby players: agreement between different methods and association with physical performance.

BACKGROUND: This study investigated the comparability between air displacement plethysmography (ADP), dual-energy X-ray absorptiometry (DXA), and bioelectrical impedance analysis (BIA) methods for body composition assessment and their correlations with physical performance in rugby players. METHODS: Nineteen male elite players participated in the study. ADP, DXA, and BIA were used to assess fat-mass and fat-free mass. Physical performance was assessed by means of Carminatti's test of peak velocity (PVTCAR), countermovement jump (CMJ), sprint speed (10 and 30-m), and match performance analyses (sprinting, distance covered, and high-intensity running). RESULTS: BIA overestimated fat-mass (13±41%; r2=0.60) and underestimated fat-free mass (-1±7%; r2=0.66) compared to ADP (P=0.001). BIA underestimated fat-mass (-28±3%; r2=0.92) and overestimated fat-free mass (10±5%; r2=0.87) compared to DXA (P<0.001). ADP underestimated fat-mass (-36±37%; r2=0.69) and overestimated fat-free mass (11±8%; r2=0.52) compared to DXA (P<0.001). Fat-mass measured by ADP, DXA, and BIA negatively correlated with PVTCAR (r2≥0.49), height and peak power from CMJ (r2≥0.30), sprinting ability (r2≥0.65), and match performance variables (r2≥0.30). CONCLUSIONS: As long as one considers that ADP and BIA underestimated fat-mass and overestimated fat-free mass compared to DXA, the methods can be used to estimate body composition, particularly to track body fat-mass changes, which negatively influence several physical capacities determinant to rugby performance. The limitations of the methods should be borne in mind when assessing the body composition of rugby athletes.

Clusters of cardiovascular risk factors and its association with muscle strength in adults.

BACKGROUND: Cardiovascular diseases are the leading cause of death globally. The aim of the study was to investigate the relationship between muscle strength (MS) and clusters of metabolic syndrome (MetS) components in adults. METHODS: Population-based cross-sectional study comprising 533 adults (45.3±11.1 years) from Florianópolis, Brazil. MS - calculated as the ratio between absolute strength (kgf) and body mass (kg) - was measured by handgrip dynamometry. Data on blood pressure, waist circumference, lipids and blood glucose were used to identify MetS components that were analyzed as individual variables and clusters. Multiple linear regression adjusted was used. RESULTS: Individually, MS was inversely associated with all MetS components (1, 2, 3 and 4 factors, ß of at least -0.08 kgf/kg). In addition, MS was negatively associated with number of positive MetS components (1, 2, 3 and 4 factors; ß of at least -0.05 kgf/kg). Individually or combined in clusters of two, three and four risk factors (ß of at least -0.20 kgf/kg), abdominal obesity was directly associated with lower MS values. All combinations of 3 and 4 MetS components (ß of at least -0.13 kgf/kg) were inversely associated with MS. CONCLUSIONS: Greater number of individual MetS components were related to lower MS values.

Deslocamento ativo para a escola e indicadores antropométricos de obesidade em adolescentes / Active commuting to school and anthropometric indicators of obesity in adolescents

Os baixos níveis de atividade física e a obesidade na população adolescente têm requerido estratégias urgentes em saúde pública, pois são potenciais fatores de risco para o desenvolvimento de doenças crônicas não transmissíveis. O objetivo deste estudo foi comparar os indicadores antropométricos de obesidade em adolescentes, considerando diferentes formas de deslocamento para a escola. Trata-se de uma pesquisa epidemiológica, de delineamento transversal, conduzida em 818 adolescentes (375 rapazes e 443 moças) com média de idade de 16,3±1,0 anos, estudantes do ensino médio de escolas públicas estaduais do município de São José-SC, Brasil. Os adolescentes informaram, por meio de questionário autoadministrado, como normalmente se deslocavam para a escola (a pé ­ considerado como deslocamento ativo ­ ou por meio de carro/moto ou ônibus ­ analisado como deslocamento passivo). Foram aferidas as medidas de massa corporal e estatura (para cálculo do IMC), perímetro da cintura e dobras cutâneas das regiões do tríceps e subescapular (considerando-se o somatório das duas dobras). Empregou-se o teste Kruskal-Wallis para comparar os indicadores antropométricos de obesidade entre os tipos de deslocamento realizados no percurso da residência para a escola. Os resultados apontaram que os adolescentes que se deslocavam ativamente para a escola apresentaram menores medidas de perímetro da cintura (p= 0,034). Entre os sexos, as menores medidas do somatório de dobras cutâneas e perímetro da cintura foram observadas, respectivamente, entre rapazes (p= 0,043) e moças (p= 0,009) que se deslocavam de maneira ativa para a escola. Conclui-se que, nos adolescentes investigados, as menores medidas da gordura corporal foram observadas entre aqueles que se deslocavam de maneira ativa, o que reforça a importância do deslocamento ativo como um hábito a ser considerado pelos escolares como um meio de melhorar seus indicadores antropométricos. Faz-se necessário, portanto, a implementação de políticas públicas que promovam o deslocamento ativo para a escola entre os adolescentes...(AU)

Obesity and low levels of physical activity among the adolescent population have been required urgent strategies in public health, since they are potential risk factors for the development of chronic non-communicable diseases. This study aimed to compare the anthropometric indicators of obesity in adolescents considering different forms of commuting to school. This is an epidemiological research, with a cross-sectional design, that included 818 adolescents (375 boys and 443 girls) with mean age of 16.3±1.0 years, who were high school students in state public schools of the city of São José-SC, Brazil. The adolescents reported, by means of a questionnaire self-administered, as they normally commuted to school (walking - considered as active commuting -or by means of car/bike or bus - considered as passive commuting). Body mass and height were measured (to calculate BMI), and also the waist circumference and skinfold thickness in the regions of the triceps and subscapular (considering the sum of the two folds). We used the Kruskal-Wallis test to compare the anthropometric indicators of obesity among the types of commuting undertaken in the course of the residence to school. The results showed that the adolescents who used active commuting to school had lower measures of waist circumference (p= 0.034). Between the sexes, smaller measures of sum of skinfolds and waist circumference were observd, respectively, among boys (p= 0.043) and girls (p= 0.009) that used active commuting to school. We concluded that, in the adolescents investigated, a healthier body composition among adolescents who used the active commuting from the residence to the school was observed, which reinforces the importance of active commuting as a behavior to be considered by the students and a way to improve levels of physical activity as well as their anthropometric indicators...(AU)

Relationship of weight gain with infrared temperatures in Nelore and F1 (Nelore × Angus) heifers reared in two forage production systems1.

Intensive production systems require high-yield genetics as obtained in Bos taurus × Bos indicus crossbreeding. Generally, high-producing taurine cattle are more susceptible to parasites and heat stress. This study evaluated animal performance, heat-stress measurement (infrared temperatures), and internal parasite infection with daily weight gain in heifers from 2 genetic groups (Nelore and F1-Nelore × Angus) reared in 2 forage production systems (with or without crop-livestock system) during 1 yr. The main objectives were to determine the relationship between infrared measures and animal performance and whether it differs between genetic groups and environments. Thirty-six heifers were randomly assigned to 2 forage production systems, one considered as high-input system with crop-livestock system and other exclusive livestock system considered as low input. At each 28 d, infrared thermography (IR) temperatures, weight, and internal parasite infection (fecal egg count) were measured. The temperatures of the eye, snout, forehead, dewlap, body, ground and squeeze chute were determined. F1 heifers had higher weight gain than Nelore (P < 0.05) and both did not differ in internal parasite infection (P > 0.05). F1 heifers had higher IR than Nelore (P < 0.05). The main body points that differentiate between genetic groups were dewlap, forehead, and eye. Higher dewlap IR temperature (DW) was associated with higher average daily gain (ADG) during dry season (independently of genetic groups) (ADG = -0.755 + 0.032 × DW; R2 = 0.44). Otherwise, the IR temperatures had a negative relationship with ADG during rainy season and low forehead IR temperature was related to higher average daily gain (ADG = 1.81 - 0.033 × forehead; R2 = 0.12 for F1 animals and ADG = 1.46 - 0.025 × forehead; R2 = 0.07 for Nelore). The infrared temperatures were more related to animal performance during the dry season, which had high temperature and low humidity. The infrared temperatures were able to identify the animal response to the environment challenge. Animals with higher temperatures (dewlap and forehead) had higher daily gain during the dry season.

Nanoparticles for Protein Sensing in Primary Containers: Interaction Analysis and Application.

Silver nanoparticles (AgNPs) are known to interact with proteins, leading to modifications of the plasmonic absorption that can be used to monitor this interaction, entailing a promising application for sensing adsorption of therapeutic proteins in primary containers. First, transmission electron microscopy in combination with plasmonic absorption and light scattering responses were used to characterize AgNPs and protein-AgNP complexes, including its concentration dependence, using two therapeutic molecules as models: a monoclonal antibody (mAb) and a synthetic copolymer (SC). Upon interaction, a protein corona was formed around AgNPs with the consequent shifting and broadening of their characteristic surface plasmon resonance (SPR) band (400 nm) to 410 nm and longer wavelenghts. Additional studies revealed secondary and three-dimensional structure modifications of model proteins upon interaction with AgNPs by circular dichroism and fluorescence techniques, respectively. Based on the modification of the SPR condition of AgNPs upon interaction with proteins, we developed a novel protein-sensing application of AgNPs in primary containers. This strategy was used to conduct a compatibility assessment of model proteins towards five commercially available prefillable glass syringe (PFS) models. mAb- and SC-exposed PFSs showed that 74 and 94% of cases were positive for protein adsorption, respectively. Interestingly, protein adsorption on 15% of total tested PFSs was negligible (below the nanogram level). Our results highlight the need of a case-by-case compatibility assessment of therapeutic proteins and their primary containers. This strategy has the potential to be easily applied on other containers and implemented during early-stage product development by pharmaceutical companies and for routine use during batch release by packaging manufacturers.