Malformation of the tail in Labrador Retriever dogs caused by mutation C189G in the T gene / Malformação da cauda em cães da raça Labrador Retriever causada por mutação C189G no gene T

The present study reported the mutation C189G in the T gene (Brachyury gene) as the cause of malformation in the tail of the Labrador dog. One litter of Labradors, from a mating between a female with short tail and a male with normal tail admitted at the Veterinary Teaching Hospital of Universidade Federal de Mato Grosso do Sul, Campo Grande, Brazil, was evaluated in this study. Blood samples were collected from the female and her puppies. After DNA extraction, sequencing and PCR-RFLP were carried out. The C189G mutation was identified through both techniques only in dogs with short tail.(AU)

No presente trabalho relata-se a mutação C189G no gene T (Brachyury gene) como causa da malformação da cauda em cães da raça Labrador. Uma ninhada de labradores, provenientes do acasalamento entre uma fêmea com a cauda curta e um macho com a cauda normal, encaminhados ao Hospital Veterinário da Universidade Federal de Mato Grosso do Sul, Campo Grande, Brasil, foi avaliada nesse estudo. Amostras de sangue da cadela e filhotes foram coletadas. Após extração de DNA, sequenciamento e PCR-RFLP foram realizados. A mutação C189G foi identificada por meio de ambas as técnicas apenas nos cães com a cauda malformada.(AU)

Teledermatology leading to an important diagnosis in an underserved clinic.

Cutaneous signs can be the first manifestation of important medical diagnoses, including inherited cancer syndromes, but access to dermatologic evaluation is especially challenging for uninsured patients. Herein, we present a case in which a volunteer academic teledermatology triage program was used by a community health clinic to make a diagnosis of multiple cutaneous leiomyomas, which confer a high likelihood of hereditary leiomyomatosis and renal cell cancer syndrome, also known as Reed syndrome; this prompted malignancy screening for the patient. Importantly, this case underscores the potential for teledermatology to improve access to dermatologist evaluation and make crucial diagnoses in patients with barriers to care.

Post-marketing surveillance of generic amoxicillin using a microbiological assay and pharmacokinetic approach in rats.

Generic medicines were developed to increase population access to health treatment, to reduce costs and to allow drugs with the same outcomes to be purchased at lower prices. They are therapeutically equivalent to their brand-name counterparts and are interchangeable with them. However, the acceptance of generic medicines by physicians and general consumers is often affected by distrust related to quality and efficacy. In this study three different brands of generic amoxicillin were tested. The results showed that two of them were indistinguishable from the innovator in terms of microbiological potency; however, generic B was unable to reach the Brazilian Pharmacopoeia specifications for potency limits. In contrast, generic B was bioequivalent to the innovator amoxicillin in pharmacokinetic assessment and, surprisingly, generic A, which was approved in the microbiological potency assay, lacked pharmacokinetic equivalence compared with the innovator. Both tests, when used singly, may not be effective at detecting quality deviations in antimicrobial medicines, which indicates that pharmacokinetic tests in rats in association with microbiological potency assays are a valuable tool for post-marketing surveillance of generic antibiotics.