ABSTRACT
The effects of bisphenol A (BPA) contamination on the cardiovascular function still are not clear. Here, we evaluated the vascular effects of BPA and the protective actions of kefir in infant rats. Animals (25 days old) were treated with BPA (100 µg/Kg/day) for 60 days (BPA group), or administered kefir (0.3 mL/100 g) in addition to BPA (BPA kefir group), compared with non-treated rats (Control group).The vascular endothelial function was evaluated in aortic rings through the relaxation response to acetylcholine and specific blockers. The balance between reactive oxygen species (ROS) and nitric oxide (NO) was assessed through flow cytometry in the vascular tissue. The BPA group developed high blood pressure (+10%) and the analysis of vascular reactivity showed an impaired ACh-induced relaxation (~80%). The further analysis by using NADPH, NOS and COX blockers revealed that the impaired vasorelaxation was due to increased ROS production (+12%), NO bioavailability (-12%) and increased vasoconstriction to prostanoids (+36%) compared with the Control group. Kefir treatment reverted those effects significantly. Analysis of the aortic cells showed increased â¢O2- production (1942±39 a.u.) and decreased NO bioavailability (1250±30 a.u.) compared with the Control group (1374±146 and 2777±25 a.u., P<.05) and kefir reverted these values (1298±57 and 2517±57 a.u.). Contamination by BPA in this model caused hypertension and endothelial dysfunction and it was accompanied by a vascular ROS/NO imbalance, damage of endothelial layer and pro-apoptotic effects. The novelty is that the treatment using probiotic kefir was able to attenuate the progression the above BPA effects.
Subject(s)
Aorta/drug effects , Benzhydryl Compounds/toxicity , Hemodynamics , Kefir , Phenols/toxicity , Reactive Oxygen Species/metabolism , Animals , Animals, Newborn , Apoptosis , Body Weight , Cardiovascular System/drug effects , Cell Survival , DNA/metabolism , Endothelium, Vascular/metabolism , Flow Cytometry , Male , Microscopy, Electron, Scanning , Oxidative Stress , Rats , Rats, WistarABSTRACT
O estudo objetiva descrever o desequilíbrio que o diagnóstico do câncer infanto-juvenil provoca nas famílias e avaliar a representatividade do diagnóstico em crianças, adolescentes e famílias envolvidas e o comportamento da equipe de enfermagem na percepção dos familiares das crianças e adolescentes acometidos. Estudo realizado na Unidade de Onco-Hematologia doHINSG, com familiares de crianças e adolescentes diagnosticados com câncer. Os resultados demonstram que 20% dos familiares vivenciaram desespero; 21%, medo da morte; 39%, solidariedade; 22%, ciúmes; 19%, desprezo; 56% sofreram alterações nasrotinas. Os impactos causados envolvem aspectos físicos, psicossociais e financeiros, causando transformações e gerando desequilíbrio no convívio social e familiar. Quanto ao comportamento da equipe de enfermagem para com os familiares, 90% se apresentam solícitos e educados em relação ao atendimento das queixas dos clientes; 75,6% dos funcionários esclarecem dúvidas e atendem a solicitações. Conclui-se que uma assistência de enfermagem pautada na humanização durante o tratamentodeste tipo de cliente, extensiva aos seus familiares, é de grande relevância.
