Chemical Characterization, Phenolic Compounds Quantification, and Assessment of Antioxidant and Anti-Acetylcholinesterase Activities of Byrsonima sericea DC fruits.

Byrsonima sericea is a species native to Brazil that is widely used in traditional medicine. The seed ethanol extract (SEE) had the highest content of total phenols (179.35 mg GAE g-1 extract) and flavonoids (10.42 mg QE g-1 extract) and was the most active in relation to antioxidant activity (DPPH: IC50 =4.25 µg mL-1 and ABTS: IC50 =4.82 µg mL-1 ). The peel/pulp ethanol extract (PEE) had the best anticholinesterase activity (IC50 =6.02 µg mL-1 ). Chromatographic investigation identified gallic acid, isoquercitrin, quercetin and amentoflavone in SEE, and gallic acid, isoquercitrin, quercetin and rutin in PEE. Six fatty acid methyl esters and seven triterpenes were identified, highlighting oleic acid in the seed hexane extract (61.85 %) and in the peel/pulp hexane extract (52.61 %), and betulin in the peel/pulp hexane extract (5.25 %). The substances and biological activities identified in B. sericea characterize this fruit as a functional food for future studies.

Ozonation for Pseudomonas paracarnis control: biofilm removal and preservation of chicken meat during refrigerated storage.

Ozone has been studied to control microorganisms in food, as well as to control biofilm. In this context, the goals of this work were to determine the effect of ozonated water in the removal of Pseudomonas paracarnis biofilm and the effect of ozone gas and ozonated water on inactivating P. paracarnis in deboned chicken breast meat and its effect on product color. AISI 304 coupons were used as a surface for biofilm formation. The coupons were immerged into minimal medium for Pseudomonas inoculated with the P. paracarnis overnight culture (1% w/v) followed by incubation at 25 °C for 7 days. To obtain ozonized water, two different systems were used: system with microbubble generator (MB) and system with porous stone diffuser (PSD). The inlet ozone concentration was 19 mg/L and flow rate of 1 L/min. The coupons were subjected to ozonized water for 10 and 20 min. The chicken breast meat was exposed to gaseous ozone and ozonized water for 40 min. After the ozonation process, chicken meat samples were stored at 8 °C, for 5 days. More expressive removals of biofilm were obtained when using ozonized water obtained in the system with microbubble generator (MB for 20 min-reduction of 2.3 log cycles) and system with porous stone diffuser (PSD for 10 min-reduction of 2.7 log cycles; PSD for 20 min-reduction of 2.6 log cycles). The treatment of chicken meat with ozone gas resulted in lower counting of Pseudomonas, when compared with the control treatments and with ozonized water, both immediately after ozonation (day 1) and after 5 days of storage. The luminosity in the chicken meat samples treated with ozonized water was higher than that verified in the control treatments and with ozone gas, immediately after ozonation (day 1). A similar trend was observed in hue angle and color difference, in which the highest values were obtained for treatment with ozonized water. Based on the results obtained in this study, it was concluded that ozonated water can be used to remove P. paracarnis biofilm from stainless steel under static conditions and gaseous ozone is more efficient in the inactivation of P. paracarnis from chicken breast meat, when compared to ozonated water.

Características sociodemográficas e sinais de depressão e ansiedade em mães/pais/cuidadores de autistas / Sociodemographic characteristics and signs of depression and anxiety in mothers/fathers/caregivers of autistic children / Características sociodemográficas y signos de depresión y ansiedad en madres/padres/cuidadores de niños autistas

Objetivo: avaliar o impacto de características sociodemográficas sobre a intensidade de sinais de depressão e ansiedade em mães/pais/cuidadores de pessoas no espectro autista. Método: estudo transversal, analítico e quantitativo, realizado com 31 mães/pais/cuidadores de pessoas no espectro autista. Aplicou-se um formulário sociodemográfico e os inventários de Depressão e de Ansiedade de Beck. A análise foi realizada por meio de estatística descritiva e regressão linear, com método backward. Resultados: verificou-se que ser natural de Caxias (MA), ter maior escolaridade e maior quantidade de horas trabalhadas semanalmente contribuem para menor intensidade de sinais de depressão e ansiedade. Outrossim, o fato de desenvolver atividades laborativas diminui as chances de depressão e praticar alguma religião demonstrou-se relevante para a diminuição de sinais de ansiedade. Conclusão: algumas características sociodemográficas contribuem positivamente para a diminuição de sinais de depressão e ansiedade em mães/pais/cuidadores de pessoas autistas, o que denota a necessidade de conhecê-las e otimizá-las.

Objective: to evaluate the impact of sociodemographic characteristics on the intensity of signs of depression and anxiety in mothers/fathers/caregivers of people on the autistic spectrum. Method: cross-sectional, analytical and quantitative study, carried out with 31 mothers/fathers/caregivers of people on the autistic spectrum. A sociodemographic form and Beck's Depression and Anxiety inventories were applied. The analysis was performed using descriptive statistics and linear regression, with the backward method. Results: it was found that being born in Caxias (MA), having higher education and more hours worked weekly contribute to lower intensity of signs of depression and anxiety. Furthermore, the fact of carrying out work activities decreases the chances of depression and practicing a religion has been shown to be relevant for the reduction of signs of anxiety. Conclusion: some sociodemographic characteristics contribute positively to the reduction of signs of depression and anxiety in mothers/fathers/caregivers of autistic people, which denotes the need to know and optimize them.

Toxicity of allyl isothiocyanate applied in systems with or without recirculation for controlling Sitophilus zeamais, Rhyzopertha dominica, and Tribolium castaneum in corn grains.

BACKGROUND: The application of allyl isothiocyanate (AITC) has been proposed as an alternative to control stored-grain insects. However, AITC is a compound with a low diffusion coefficient, making its distribution throughout the grain mass difficult. Therefore, the objective of the present study was to evaluate the effectiveness of AITC applied in systems with or without recirculation for controlling Sitophilus zeamais (Mots. 1855) (Coleoptera: Curculionidae), Rhyzopertha dominica (Fabr.) (Coleoptera: Bostrichidae), and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) infesting a corn grain mass. The assays used a polyvinyl chloride (PVC) prototype, dimensioned 1.60 m in length, 0.30 m in diameter, and a static capacity of 60 kg of grains. AITC toxicity to insects was evaluated at the base, 0.5 m from the base, and top of the grain column (1.0 m). Different concentrations of AITC were tested for an exposure period of 48 h. RESULTS: In the system without AITC recirculation, insect mortality was verified only at the base of the grain column. However, insect mortality was considered uniform at the different positions of the column when the AITC recirculation system was adopted. In this system, there was also a marked reduction in the instantaneous population growth rate of S. zeamais, T. castaneum, and R. dominica, and a decrease in the dry matter loss of the grains, when the AITC concentrations were increased. CONCLUSION: AITC recirculation proved to be a viable strategy for protecting grains against the species S. zeamais, R. dominica, and T. castaneum. AITC fumigation ultimately did not cause changes in grain quality. © 2023 Society of Chemical Industry.

Effect of preheating on mechanical properties of a resin-based composite containing elastomeric urethane monomer.

This study investigated the effect of preheating an elastomeric urethane monomer (Exothane-24) experimental resin composite on its physicochemical properties. Two resin matrices were formulated: (a) 50 wt% Bisphenol-glycidyl methacrylate (Bis-GMA) and 50 wt% triethylene glycol dimethacrylate (TEGDMA); and (b) 20 wt% Exothane-24, 40 wt% Bis-GMA and 40 wt% TEGDMA. A photoinitiator system (0.25 wt% camphorquinone and 0.50 wt% ethyl-4-dimethylamino benzoate) and 65 wt% of the inorganic filler (20 wt% 0.05 µm silica and 80 wt% 0.7 µm BaBSiO2 glass) were added to both matrices. These formulations were then assigned to four groups: Exothane-24 (E); Exothane-24 plus preheating (EH); no Exothane-24 (NE); and no Exothane-24 plus preheating (NEH). NEH and EH were preheated at 69 °C. The dependent variables were as follows: film thickness (FT); polymerization shrinkage stress (PSS); gap width (GW); maximum rate of polymerization (Rpmax); and degree of conversion (DC). Data were statistically analyzed by two-way ANOVA and Tukey's test (α = 0.05). Preheating reduced FT for both composites. PSS and GW were significantly lower for EH, when compared with E. The DC for EH and NEH and the Rpmax for EH increased significantly. Preheating improved most of the physicochemical properties (FT, PSS, GW, and DC) of the experimental resin composite containing Exothane-24.

Fatores associados à qualidade nos relacionamentos interpessoais de acadêmicos de enfermagem / Factors associated with quality in interpersonal relationships of nursing academics / Factores asociados a la calidad en las relaciones interpersonales de los académicos de enfermería

Objetivo: verificar os fatores associados à alta tolerância nas relações de amizade durante o período acadêmico. Métodos: estudo transversal, analítico, realizado com 151 discentes de enfermagem de duas instituições de ensino superior privadas com coleta de dados sociodemográficos e aplicação de instrumento de avaliação da tolerância nas relações de amizade. Realizada análise por meio de estatística descritiva e regressão linear, com método backward. Resultados: verifica-se a predominância de discentes do sexo feminino, solteiras e católicas. Os discentes possuem idade média de 22,8 anos e 1,3 filhos. Reconhecer que é difícil conviver consigo; pedir desculpas, mesmo não estando errado; e acreditar que a amizade é aceitar a outra pessoa do jeito que ela é, foram fatores contribuintes para a alta tolerância. Além disso, o número de filhos contribui para o aumento da tolerância nas relações de amizade. Conclusão: a alta tolerância nas relações de amizade é importante não só para a permanência nos cursos de enfermagem, mas para o estabelecimento de vínculos interpessoais efetivos, o que reflete no comportamento em equipe, pois está relacionada à interdependência e multidisciplinaridade profissional contribuindo para a prestação de cuidado nos diversos serviços de saúde. (AU)

Objective: to verify the factors associated with high tolerance in friendships during the academic period. Methods: cross-sectional, analytical study carried out with 151 nursing students from two private educational institutions with collection of sociodemographic data and application of an instrument for assessing tolerance in friendship relationships. Analysis performed using descriptive statistics and linear regression, using the backward method. Results: there is a predominance of female, single and Catholic students. The students have an average age of 22.8 years and 1.3 children. Recognizing that it is difficult to live with you, apologizing even if you are not wrong and believing that friendship is accepting the other person as they are were contributing factors to high tolerance. In addition, the number of children contributes to increasing tolerance in friendly relationships. Conclusion: the high tolerance in friendship relationships is important not only for staying in nursing courses, but for establishing effective interpersonal bonds, which reflects in team behavior because it is related to professional interdependence and multidisciplinarity contributing to the provision of care in the various health services. (AU)

Objetivo: verificar los factores asociados a la alta tolerancia en las amistades durante el período académico. Métodos: estudio transversal, analítico realizado con 151 estudiantes de enfermería de dos instituciones educativas privadas con recolección de datos sociodemográficos y aplicación de un instrumento para evaluar la tolerancia en las relaciones de amistad. Análisis realizado mediante estadística descriptiva y regresión lineal, utilizando el método hacia atrás. Resultados: hay predominio de estudiantes mujeres, solteras y católicas. Los alumnos tienen una edad media de 22,8 años y 1,3 hijos. Reconocer que es difícil vivir consigo; pedir perdón, aunque no te equivoques; y creer que la amistad es aceptar a la otra persona como es, fueron factores que contribuyeron a una alta tolerancia. Además, el número de hijos contribuye a aumentar la tolerancia en las relaciones amistosas. Conclusión: la alta tolerancia en las relaciones de amistad es importante no solo para la permanencia en los cursos de enfermería, sino para establecer vínculos interpersonales efectivos, se refleja en el comportamiento del equipo, puesto que se relaciona con la interdependencia profesional y la multidisciplinariedad contribuyendo a la prestación de cuidados en los distintos servicios de salud. (AU)

Psychometric properties and measurement invariance of the Rigid and Flexible Persistence Scale in a Brazilian professional sample.

Persistence involves the intention to maintain efforts when faced with obstacles and challenges, in order to achieve a specific goal. The Rigid and Flexible Persistence Scale (RFPS) is a self-report measure that assesses persistence that is theoretically derived from the premises of the Dualistic Model of Passion. The aim of the present research was to adapt the RFPS to Brazilian Portuguese and to estimate its evidence of validity and reliability in the context of work. Participants were 400 professionals, 55.8% women, aged between 18 and 68 years. The results indicate that the RFPS presented validity evidence based on the content. Corresponding to the theoretical expectations, exploratory and confirmatory factor analyses demonstrated adequacy of the structure composed of two factors, explaining 75% of the data variance, with good levels of reliability. The RFPS also revealed strong invariance across gender and work conditions (in-person vs remote). Flexible persistence showed positive association with harmonious passion and occupational self-efficacy. Conversely, rigid persistence showed positive association with obsessive passion. It was observed a mediational role of occupational self-efficacy in the relationship between harmonious passion and flexible persistence. Overall, the findings suggest that the RFPS is an adequate measure of persistence in a Brazilian occupational sample.

Social support and professional networks of nurses and nursing technicians in coping with Covid-19: A sectional study in two Brazilian cities.

Among healthcare workers, nurses are at exceptionally considerable risk for contracting COVID-19. Regardless of professionals' level of education, Brazilian nursing is one of the healthcare occupations shouldering the highest levels of responsibility and workload. Social support networks to health and nursing can be a strategy to reduce workload and stress and may contribute to implementing the activities and protecting workers' health. This study aimed to map and analyze social support networks at workplaces as informed by frontline nursing professionals working in healthcare units in the Brazilian cities of Rio de Janeiro and Fortaleza, capitals of the States of Rio de Janeiro and Ceará, respectively. This observational and cross-sectional study used an online data collection instrument based on social network analysis methodology. We recruited 163 participants in two reference services for health professionals suspected or with COVID-19 symptoms. The research question was: "Which category or categories of health professionals have supported you or other colleagues the most in the event of diagnosis or suspicion of COVID-19 among nursing categories? Data were organized by nursing category and city and analyzed through social network analysis using Ucinet©, generating graphs and centrality metrics. Results point to the central relevance of nursing categories in the workplace social support in the pandemic, followed by other health professional categories.

Low-pressure ozone injection system: relationship between reaction kinetics and physical properties of grains.

BACKGROUND: The ozonation of grains in a closed system at low pressure is a strategy with the potential for treating packaged products. Research is necessary to characterize the reaction kinetics of ozone in this type of injection system so that it is possible to design chambers and determine the ozone concentrations suitable for commercial-scale applications. The objective of this study was therefore to characterize the low-pressure ozone injection system in relation to the physical properties of the grains and determine possible changes in their quality. Samples (5 kg each) of common beans, cowpea beans, corn, popcorn kernels, paddy rice, and polished rice were exposed to ozone in a 70 L hypobaric chamber. Initially, the internal pressure of the chamber was reduced to 500 hPa. Then, ozone was injected at a concentration of 32.10 g m-3 at a volumetric flow rate of 1 L min-1 until reaching a pressure of 1000 hPa. To relate the decomposition of ozone to the grains that were being evaluated, different physical properties were determined, and quality analysis was conducted. RESULTS: Ozone gas half-life outside and inside the package depended on the grain type. Ozone decomposition was quickest in polished rice and slowest in common beans. The half-life of the different grains ranged from 17.8 to 52.9 and 16.4 to 52.9 min, outside and inside the package, respectively. Considering the physical properties, specific surface (Ss), surface area (SA), and sphericity (φ) exhibited a significant correlation with the decomposition rate constant (k) of ozone. However, the variables volume (V), permeability (K), porosity (ε), and specific mass (ρ) showed no correlation with k. CONCLUSION: The physical properties of grain influenced the reaction kinetics of ozone gas during the low-pressure injection process. Ozone gas injection at low pressures did not alter the quality attributes of the grains under study. © 2022 Society of Chemical Industry.

Psychometric properties and measurement invariance of the Rigid and Flexible Persistence Scale in a Brazilian professional sample

Abstract Persistence involves the intention to maintain efforts when faced with obstacles and challenges, in order to achieve a specific goal. The Rigid and Flexible Persistence Scale (RFPS) is a self-report measure that assesses persistence that is theoretically derived from the premises of the Dualistic Model of Passion. The aim of the present research was to adapt the RFPS to Brazilian Portuguese and to estimate its evidence of validity and reliability in the context of work. Participants were 400 professionals, 55.8% women, aged between 18 and 68 years. The results indicate that the RFPS presented validity evidence based on the content. Corresponding to the theoretical expectations, exploratory and confirmatory factor analyses demonstrated adequacy of the structure composed of two factors, explaining 75% of the data variance, with good levels of reliability. The RFPS also revealed strong invariance across gender and work conditions (in-person vs remote). Flexible persistence showed positive association with harmonious passion and occupational self-efficacy. Conversely, rigid persistence showed positive association with obsessive passion. It was observed a mediational role of occupational self-efficacy in the relationship between harmonious passion and flexible persistence. Overall, the findings suggest that the RFPS is an adequate measure of persistence in a Brazilian occupational sample.

Resolutividade no Subsistema de Atenção à Saúde Indígena no Brasil: revisão de escopo / Resolubility in the Indigenous Health Care Subsystem in Brazil: scope review / Resolución en el Subsistema de Atención de la Salud Indígena en Brasil: revisión del alcance

RESUMO: A resolutividade relaciona-se à capacidade de solução dos problemas de saúde nos serviços. Em 1999, o Subsistema de Atenção à Saúde Indígena foi integrado ao Sistema Único de Saúde no Brasil, passando a seguir os seus princípios e diretrizes. Este estudo teve por objetivo identificar e mapear os desafios ou problemas relacionados às práticas em saúde para a resolutividade no Subsistema de Saúde Indígena após a integração. Trata-se de uma revisão de escopo que utilizou seis bases de dados nacionais e internacionais. Os estudos elegíveis tiveram como critério base o mnemônico PCC (P: população indígena; C: desafios ou problemas para a resolutividade; C: subsistema de saúde indígena brasileiro). Foram encontrados 1.748 estudos e selecionados 33, com predomínio de estudos qualitativos. Os desafios ou problemas sensíveis para o processo da resolutividade foram encontrados nos aspectos que tangem à educação em saúde, à interculturalidade, ao acesso universal e aos recursos em gestão. O saber tradicional é pouco valorizado pelo sistema de saúde. A deficiência de recursos humanos e materiais, a falta de efetiva educação permanente e de capacitações para trabalhar no contexto intercultural produzem barreiras de acesso e comprometem a resolutividade nos serviços, aumentando assim as iniquidades em saúde.

ABSTRACT: Resolubility relates to the ability to solve health problems in services. In 1999, the Indigenous Health Care Subsystem was integrated into the Brazilian Unified Health System, following its principles and guidelines. The objective of this study was to identify and map the challenges or problems related to health practices for solving in the Indigenous Health Subsystem after integration. This is a scope review that used six national and international databases. Eligible studies were based on mnemonic PCC (P: indigenous population; C: challenges or problems for resolution; C: Brazilian indigenous health subsystem). A total of 1,748 studies were found and 33 were selected, with predominance of qualitative studies. The challenges or problems that are sensitive to the resolution process were found in the aspects that are related to health education, interculturality, universal access and management resources. Traditional knowledge is underrated by the health system. The deficiency of human and material resources, the lack of effective permanent education and capacitations to work in the intercultural context, produce barriers to access and compromise the resolubility in services, thus increasing the inequities in health.

RESUMEN: La resolución se refiere a la capacidad de resolver problemas de salud en los servicios. En 1999, el Subsistema de Atención de Salud Indígena se integró en el Sistema Único de Salud de Brasil, siguiendo sus principios y directrices. El objetivo de este estudio fue identificar y mapear los desafíos o problemas relacionados con las prácticas de salud para resolver en el Subsistema de Salud Indígena después de la integración. Esta es una revisión de alcance que utilizó seis bases de datos nacionales e internacionales. Los estudios elegibles se basaron en PCC mnemónicos (P: población indígena; C: desafíos o problemas para la resolución; C: subsistema de salud indígena brasileño). Se encontraron 1.748 estudios y se seleccionaron 33, con predominio de estudios cualitativos. Los desafíos o problemas que son sensibles al proceso de resolución se encontraron en los aspectos que están relacionados con la educación en salud, la interculturalidad, el acceso universal y los recursos de gestión. El conocimiento tradicional es subestimado por el sistema de salud. La deficiencia de recursos humanos y materiales, la falta de educación permanente efectiva y de capacitaciones para trabajar en el contexto intercultural, producen barreras para acceder y comprometer la solubilidad en los servicios, aumentando así las desigualdades en salud.

Notificações emitidas pelo Conselho Regional de Enfermagem do Paraná / Notifications issued by the Paraná Regional Nursing Council / Notificaciones emitidas por el Consejo Regional de Enfermería de Paraná

Objetivo: Levantar e caracterizar as notificações de irregularidades e ilegalidades emitidas pelo Conselho Regional de Enfermagem do Paraná (COREN-PR) entre os anos de 2018 a 2022. Método:Pesquisa documental, com abordagem quantitativa, tendo o COREN-PR como cenário de pesquisa. Foram coletados dados de fonte primária e de domínio público do COREN-PR referentes aos relatórios de fiscalização em que constam os quantitativos de notificações por irregularidades e ilegalidades emitidas entre os anos de 2018 a 2022. Os dados foram analisados mediante análise estatística simples. Resultados:Verificou-se prevalência das notificações de inexistência ou inadequação de documentos relacionados ao gerenciamento dos processos de trabalho do serviço de Enfermagem, inexistência de anotação de responsabilidade técnica do serviço de enfermagem, profissionais de Enfermagem que não registram o processo de Enfermagem contemplando as cinco etapas preconizadas, inexistência ou inadequação dos registros relativos à assistência de enfermagem, inexistência ou ausência de enfermeiro onde são desenvolvidas as atividades de enfermagem e exercício irregular da enfermagem. Conclusão:Conhecer o perfil das notificações emitidas pelo Conselho de Classe representa um norteador para os profissionais de enfermagem direcionarem a sua atuação, em consonância com a legislação de enfermagem vigente, com vistas a um cuidado de enfermagem seguro, ético e qualificado.

Objective:To survey and characterize the notifications of irregularities and illegalities issued by the Paraná Regional Nursing Council (COREN-PR) between the years 2018 to 2022. Method:Documentary research, with a quantitative approach, with COREN-PR as the research scenario. Data were collected from a primary source and from the public domain of COREN-PR regarding inspection reports containing the number of notifications for irregularities and illegalities issued between the years 2018 to 2022. The data were analyzed using simple statistical analysis. Results:There was a prevalence of notifications of non-existence or inadequacy of documents related to the management of work processes in the Nursing service, lack of notes on the technical responsibility of the nursing service, Nursing professionals who do not register the Nursing process contemplating the five recommended steps, non-existence or inadequacy of records related to nursing care, non-existence or absence of a nurse where nursing activities are carried out and irregular nursing practice. Conclusion:Knowing the profile of notifications issued by the Class Council represents a guide for nursing professionals to direct their actions, in line with current nursing legislation, with a view to safe, ethical and qualified nursing care.

Objetivo:Relevar y caracterizar las notificaciones de irregularidades e ilegalidades emitidas por el Consejo Regional de Enfermería de Paraná (COREN-PR) entre los años 2018 a 2022. Método:Investigación documental, con enfoque cuantitativo, teniendo como escenariode investigación el COREN-PR. Los datos fueron recopilados de fuente primaria y del dominio público de COREN-PR sobre informes de inspección que contienen el número de notificaciones de irregularidades e ilegalidades emitidas entre los años 2018 a 2022. Los datos fueron analizados mediante análisis estadístico simple. Resultados:Hubo predominio de notificaciones de inexistencia o insuficiencia de documentos relacionados con la gestión de procesos de trabajo en el servicio de Enfermería, falta de notas de responsabilidad técnica para el servicio de enfermería, Profesionales de enfermería que no registran el proceso de Enfermería contemplando los cinco pasos recomendados, inexistencia o insuficiencia de registros relacionados con los cuidados de enfermería, inexistencia o ausencia de enfermero en el lugar donde se desarrollan las actividades de enfermería y práctica irregular de enfermería. Conclusión:Conocer el perfil de las notificaciones emitidas por el Consejo de Clase representa una guía para que los profesionales de enfermería orienten sus acciones, de acuerdo con la legislación de enfermería vigente, con miras a una atención de enfermería segura, ética y calificada.

Avaliação do contrafluxo: Um novo conceito como indicador de qualidade de um serviço de atendimento a vítimas de queimadura / Evaluating counterflow: A new concept as a quality indicator for a burn care service

OBJETIVO: Descrever o perfil de pacientes adultos internados por queimaduras que passaram por contrafluxo, retorno do paciente para uma unidade de maior complexidade assistencial por complicações ou deterioração clínica, durante a internação hospitalar e avaliar a relação existente entre a sua ocorrência, o Abbreviated Burn Severity Index (ABSI) e óbito. MÉTODO: Estudo retrospectivo de coorte aberta com dados secundários de um Centro de Tratamento de Queimados (CTQ) na Bahia, de janeiro de 2022 a junho de 2023. Os dados foram organizados no Excel, exportados e analisados no Stata. A significância estatística foi avaliada pelo teste Qui-quadrado ou exato de Fisher para variáveis categóricas. Este estudo foi aprovado pelo Comitê de Ética em Pesquisa. RESULTADOS: Das 658 internações no CTQ, 72 (10,9%) tiveram contrafluxo. Destas, 51,4% foram do sexo masculino, 68% estavam na faixa etária de 18 a 59 anos, predominando as queimaduras por calor/fogo. As unidades que mais receberam o contrafluxo foram a Unidade de Terapia Intensiva (UTI) para queimados (UAC), com 48 (66,7%), e UTIs Gerais, com 21 eventos (29,2%). Na população estudada, houve significância estatística na taxa de mortalidade, na taxa de contrafluxo segundo as faixas de gravidade e no contrafluxo e mortalidade segundo procedência, no qual foi identificado que os pacientes vindos da emergência sofreram contrafluxo e tiveram maior taxa de mortalidade. CONCLUSÕES: O contrafluxo representa um importante indicador a ser monitorado no CTQ em estudo, pois esteve relacionado com pior prognóstico em adultos grandes queimados, procedentes da emergência e vítimas de queimaduras térmicas.

OBJECTIVE: To describe the profile of adult patients hospitalized for burns that underwent contraflow, return the patient to a more complex care unit due to complications or clinical complications, during hospital stay and evaluate the relationship between their occurrence, the Abbreviated Burn Severity Index (ABSI) and death. METHODS: Retrospective open cohort study with secondary data from a Burn Treatment Center (BTC) in Bahia, from January 2022 to June 2023. Data were organized in Excel, exported and applied to Stata. Statistical significance was assessed using the Chi-square or Fisher's exact test for categorical variations. This study was approved by the Research Ethics Committee. RESULTS: Of the 658 admissions to the BTC, 72 (10.9%) had contraflow. Of these, 51.4% were male, 68% were aged between 18 and 59 years, with heat/fire burns predominating. The units that received the most contraflow were the burn ICU (UAC) with 48 (66.7%), and General ICUs with 21 events (29.2%). In the studied population, there was statistical significance in the mortality rate, in the contraflow rate according to the severity ranges and in the contraflow and mortality according to origin, where it was identified that patients coming from the emergency suffered contraflow and had higher mortality rates. CONCLUSIONS: Contraflow represents an important indicator to be monitored in the BTC under study, as it was related to worse prognoses in severely burned adults, those coming to emergencies and victims of thermal burns.

Relações entre Paixão pelo Trabalho, Bem-Estar e Autoeficácia Ocupacional em Treinadores Esportivos / Relationship between Passion for Work, Well-Being and Occupational Self-Efficiency in Sports Coaches / Relaciones entre la pasión por el trabajo, el bienestar y la autoeficiencia laboral en los entrenadores deportivos

O objetivo deste estudo foi verificar as associações entre a paixão pelo trabalho, bem-estar e autoeficácia ocupacional em treinadores esportivos. Participaram do estudo 139 treinadores de ambos os sexos (71,2% masculino). Foram observados efeitos positivos da paixão harmoniosa sobre afetos positivos e autoeficácia ocupacional, e efeitos negativos sobre afetos negativos. A paixão obsessiva predisse positivamente os afetos negativos. Ainda, identificou-se o papel mediador dos afetos positivos na relação entre paixão harmoniosa e autoeficácia ocupacional. Corroborando as hipóteses teóricas, os resultados sugerem a importância do envolvimento harmonioso com a atividade profissional, seu potencial sobre experiências de emoções positivas e percepção de competência para o enfrentamento dos desafios profissionais.

The aim of this study was to verify the associations between passion for work, well-being and occupational self-efficacy in sports coaches. 139 coaches of both sexes participated (71.2% male). Positive effects of harmonious passion have been observed on positive affects and occupational self-efficacy, and negative effects on negative affects. Obsessive passion positively predicted negative affects. Still, the mediating role of positive affects in the relationship between harmonious passion and occupational self-efficacy was identified. Corroborating the theoretical hypotheses, the results suggest the importance of harmonious involvement with professional activity, its potential on positive emotional experiences and perception of competence to face professional challenges.

El objetivo de este estudio fue verificar las asociaciones entre la pasión por el trabajo, el bienestar y la autoeficacia laboral en entrenadores deportivos. Participaron 139 entrenadores de ambos sexos (71,2% hombres). Se han observado efectos positivos de la pasión armoniosa sobre los afectos positivos y la autoeficacia ocupacional, y efectos negativos sobre los afectos negativos. La pasión obsesiva predijo positivamente los afectos negativos. Aún así, se identificó el papel mediador de los afectos positivos en la relación entre la pasión armoniosa y la autoeficacia ocupacional. Corroborando las hipótesis teóricas, los resultados apuntan a la importancia de la implicación armónica con la actividad profesional, su potencialidad sobre experiencias emocionales positivas y percepción de competencia para afrontar retos profesionales.

Treatment of experimentally induced partial-thickness burns in rats with different silver-impregnated dressings.

PURPOSE: To evaluate the morphometric, macroscopic and microscopic aspects of experimentally induced partial-thickness burns in rats treated with different silver-based dressings. METHODS: Wistar rats were used, divided into six treatments: saline (NaCl 0.9%); silver sulfadiazine 1%; Silvercel; Mepilex Ag; Aquacel Ag and Acticoat. The animals were monitored daily and euthanized at 7, 14 and 30 days after injury induction (DAI). RESULTS: At 7 DAI, necrosis/crust was greater in control, silver sulfadiazine and Mepilex Ag treatments, granulation tissue was induced by Aquacel Ag, polymorphonuclear infiltrate (PMN) infiltration was intensified by Mepilex Ag; mononuclear infiltrate (MN) infiltration and angiogenesis were increased by Silvercel. At 14 DAI, hemorrhage was decreased by Silvercel and Mepilex Ag, PMN infiltration increased by Acticoat. At 30 DAI, angiogenesis was greater in the Acticoat treatment and fibroblasts were increased by Acticoat and Mepilex Ag. Collagen was induced at 14 DAI by silver sulfadiazine and Aquacel Ag and, at 30 DAI, by silver sulfadiazine and Silvercel treatments. CONCLUSIONS: Silvercel and Acticoat presented better results than the other products. However, all the dressings were better than the control at some point during the process, and may contribute to the healing of partial thickness burns. Silvercel and Aquacel Ag treatments induced better cosmetic outcomes regarding wound closure and scarring.

Sponges as libraries: Increase in microplastics in Cinachyrella alloclada after 36 years.

Many studies investigated the presence and effects of microplastics in marine species, but data about sponges are still incipient. We quantified these pollutants in a population of the tropical sponge Cinachyrella alloclada, comparing museum specimens sampled in 1981 with specimens sampled in 2017. The mean number of microplastics in specimens collected decades ago was one order of magnitude lower (0.13 ± 0.40/g of sponge tissue) than those sampled more recently (1.37 ± 0.94/g). We observed microplastics in only 10 % of the specimens collected in 1981 but in 80 % of those from 2017. According to Raman spectroscopy, fibers in C. alloclada consisted of polypropylene. Our results reinforce the importance of natural history collections to reduce the gap of knowledge on the interaction between marine sponges and microplastics.

Immunogenicity of SARS-CoV-2 Trimeric Spike Protein Associated to Poly(I:C) Plus Alum.

The SARS-CoV-2 pandemic has had a social and economic impact worldwide, and vaccination is an efficient strategy for diminishing those damages. New adjuvant formulations are required for the high vaccine demands, especially adjuvant formulations that induce a Th1 phenotype. Herein we assess a vaccination strategy using a combination of Alum and polyinosinic:polycytidylic acid [Poly(I:C)] adjuvants plus the SARS-CoV-2 spike protein in a prefusion trimeric conformation by an intradermal (ID) route. We found high levels of IgG anti-spike antibodies in the serum by enzyme linked immunosorbent assay (ELISA) and high neutralizing titers against SARS-CoV-2 in vitro by neutralization assay, after two or three immunizations. By evaluating the production of IgG subtypes, as expected, we found that formulations containing Poly(I:C) induced IgG2a whereas Alum did not. The combination of these two adjuvants induced high levels of both IgG1 and IgG2a. In addition, cellular immune responses of CD4+ and CD8+ T cells producing interferon-gamma were equivalent, demonstrating that the Alum + Poly(I:C) combination supported a Th1 profile. Based on the high neutralizing titers, we evaluated B cells in the germinal centers, which are specific for receptor-binding domain (RBD) and spike, and observed that more positive B cells were induced upon the Alum + Poly(I:C) combination. Moreover, these B cells produced antibodies against both RBD and non-RBD sites. We also studied the impact of this vaccination preparation [spike protein with Alum + Poly(I:C)] in the lungs of mice challenged with inactivated SARS-CoV-2 virus. We found a production of IgG, but not IgA, and a reduction in neutrophil recruitment in the bronchoalveolar lavage fluid (BALF) of mice, suggesting that our immunization scheme reduced lung inflammation. Altogether, our data suggest that Alum and Poly(I:C) together is a possible adjuvant combination for vaccines against SARS-CoV-2 by the intradermal route.

Sleep quality in COPD patients: correlation with disease severity and health status.

OBJECTIVE: To evaluate clinical predictors of poor sleep quality in COPD patients with and without obstructive sleep apnea (OSA). METHODS: Consecutive stable patients with COPD were evaluated for OSA by means of overnight polysomnography; for sleep quality by means of the Pittsburgh Sleep Quality Index (PSQI); and for disease impact by means of the COPD Assessment Test. COPD severity was graded in accordance with the 2020 GOLD guidelines. Predictors of poor sleep quality were evaluated by multivariate logistic regression analysis. RESULTS: We studied 51 patients with COPD alone and 51 patients with COPD and OSA. Both groups had similar age (66.2 ± 9.2 years vs. 69.6 ± 10.7, p = 0.09) and airflow limitation (p = 0.37). Poor sleep quality was present in 74.8% of the study participants, with no significant difference between COPD patients with and without OSA regarding PSQI scores (p = 0.73). Polysomnography showed increased stage 1 non-rapid eye movement sleep and arousal index, as well as reduced sleep efficiency and stage 3 non-rapid eye movement sleep, in the group of patients with COPD and OSA (p < 0.05). Independent predictors of poor sleep quality were GOLD grade C/D COPD (OR = 6.4; 95% CI, 1.79-23.3; p < 0.01), a COPD Assessment Test score ≥ 10 (OR = 12.3; 95% CI, 4.1-36.5; p < 0.01), and lowest SaO2 < 80% (p < 0.0001). CONCLUSIONS: Poor sleep quality is quite common in patients with COPD and is associated with severe COPD and poor health status, having a negative impact on overall quality of life. Despite changes in polysomnography, OSA appears to have no impact on subjective sleep quality in COPD patients.

IL-6, IL-1ß and MDA correlate with Thrombolysis in Myocardial Infarction (TIMI) risk score in patients with Acute Coronary Syndrome.

BACKGROUND: Inflammation plays a significant role in the pathophysiology of Acute Coronary Syndrome (ACS) but is not included in current risk stratification. OBJECTIVE: To determine the association between Thrombolysis in Myocardial Infarction (TIMI) risk score and inflammatory biomarkers in the ACS, including unstable angina (UA), non-ST segment elevation myocardial infarction (NSTEMI), and ST-segment elevation myocardial infarction (STEMI). We hypothesized that including inflammatory biomarkers could add prognostic value to the TIMI risk score. METHODS: In this cross-sectional study, serum levels of interleukins (IL)-6 and IL-1ß and MDA (malondialdehyde) were quantified by ELISA and colorimetry, respectively , patients with ACS (n = 48; 31.3% with UA, 33.3% with NSTEMI, and 35.4% with STEMI) and healthy controls (n = 43). We assessed the TIMI scores in the first 24 h after symptom onset. RESULTS: The results showed that patients with ACS had significantly higher levels (p<0.05) of the inflammatory biomarkers IL-6, IL-1ß, and MDA compared to the control group. However, we found no significant differences in IL-6, IL-1ß, and MDA levels among the patients with ACS according to their classification as UA, NSTEMI, and STEMI. Positive correlations were observed between TIMI and IL-6 (r=0.68), IL-1ß (r= 0.53), and MDA (r=0.58) in patients with UA and between TIMI and IL-1ß (r= 0.62) in STEMI patients. CONCLUSION: These data suggest the presence of a pro-inflammatory profile in patients with ACS as well as positive correlations between TIMI scores and the inflammatory biomarkers IL-6, IL-1ß, and MDA in patients with UA and between TIMI scores and IL-1ß in patients with STEMI. Combining inflammatory biomarkers with the TIMI risk score could provide better insight into the processes involved in ACS.

Treatment of experimentally induced partial-thickness burns in rats with different silver-impregnated dressings

Purpose: To evaluate the morphometric, macroscopic and microscopic aspects of experimentally induced partial-thickness burns in rats treated with different silver-based dressings. Methods: Wistar rats were used, divided into six treatments: saline (NaCl 0.9%); silver sulfadiazine 1%; Silvercel; Mepilex Ag; Aquacel Ag and Acticoat. The animals were monitored daily and euthanized at 7, 14 and 30 days after injury induction (DAI). Results: At 7 DAI, necrosis/crust was greater in control, silver sulfadiazine and Mepilex Ag treatments, granulation tissue was induced by Aquacel Ag, polymorphonuclear infiltrate (PMN) infiltration was intensified by Mepilex Ag; mononuclear infiltrate (MN) infiltration and angiogenesis were increased by Silvercel. At 14 DAI, hemorrhage was decreased by Silvercel and Mepilex Ag, PMN infiltration increased by Acticoat. At 30 DAI, angiogenesis was greater in the Acticoat treatment and fibroblasts were increased by Acticoat and Mepilex Ag. Collagen was induced at 14 DAI by silver sulfadiazine and Aquacel Ag and, at 30 DAI, by silver sulfadiazine and Silvercel treatments. Conclusions: Silvercel and Acticoat presented better results than the other products. However, all the dressings were better than the control at some point during the process, and may contribute to the healing of partial thickness burns. Silvercel and Aquacel Ag treatments induced better cosmetic outcomes regarding wound closure and scarring.