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1.
Rev. Inst. Med. Trop. Säo Paulo ; 46(6): 315-322, Nov.-Dec. 2004. ilus, mapas, tab, graf
Article in English | LILACS | ID: lil-391587

ABSTRACT

A partir de outubro de 2001, o Instituto Adolfo Lutz tem recebido amostras de líquido vesicular e crostas de lesões de pele de pacientes das regiões do Vale do Paraíba, Estado de São Paulo e do Vale do São Patricio, Estado de Goiás. Os dados clínicos e epidemiológicos sugeriam que os surtos poderiam ser causados por Cowpox virus ou Vaccinia virus. A maioria dos pacientes era ordenhadores que tinham lesões vesicopustulares nas mãos, braços, antebraços e alguns na face. A análise por microscopia eletrônica direta (MED) detectou partículas com morfologia de vírus do gênero Orthopoxvirus em amostras de 49 (66,21%) pacientes dos 74 analisados. Os vírus foram isolados em membrana corioalantóide (MCA) de ovo embrionado de galinha e em linhagem celular Vero com confirmação por MED e PCR. Das 21 amostras de lesões submetidas ao PCR utilizando iniciadores para o gene da hemaglutinina (HA), 19 foram positivas. A digestão por enzima de restrição TaqI resultou em quatro fragmentos característicos de Vaccinia virus. A análise nucleotídica do seqüenciamento revelou que esses vírus apresentam 99,9% de similaridade com o Cantagalo virus, descrito como uma cepa de Vaccinia virus, havendo apenas alteração de um nucleotídeo na posição 616 com mudança de um aminoácido na proteína na posição 206. A análise filogenética mostrou que os isolados se agruparam junto aos Cantagalo virus, outras cepas de Vaccinia virus e Rabbitpox virus.


Subject(s)
Humans , Animals , Male , Female , Child , Adolescent , Adult , Middle Aged , Chick Embryo , Disease Outbreaks , Vaccinia , Vaccinia virus , Age Distribution , Base Sequence , Brazil , Chlorocebus aethiops , Genes, Viral , Microscopy, Electron , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Vaccinia virus , Vero Cells
2.
Rev Inst Med Trop Sao Paulo ; 46(6): 315-22, 2004.
Article in English | MEDLINE | ID: mdl-15654477

ABSTRACT

Since October 2001, the Adolfo Lutz Institute has been receiving vesicular fluids and scab specimens of patients from Paraíba Valley region in the São Paulo and Minas Gerais States and from São Patricio Valley, in the Goiás State. Epidemiological data suggested that the outbreaks were caused by Cowpox virus or Vaccinia virus. Most of the patients are dairy milkers that had vesiculo-pustular lesions on the hands, arms, forearms, and some of them, on the face. Virus particles with orthopoxvirus morphology were detected by direct electron microscopy (DEM) in samples of 49 (66.21%) patients of a total of 74 analyzed. Viruses were isolated in Vero cell culture and on chorioallantoic membrane (CAM) of embryonated chicken eggs. Among 21 samples submitted to PCR using primers for hemagglutinin (HA) gene, 19 were positive. Restriction digestion with TaqI resulted in four characteristic Vaccinia virus fragments. HA nucleotide sequences showed 99.9% similarity with Cantagalo virus, described as a strain of Vaccinia virus. The only difference observed was the substitution of one nucleotide in the position 616 leading to change in one amino acid of the protein in the position 206. The phylogenetic analysis showed that the isolates clustered together with Cantagalo virus, other Vaccinia strains and Rabbitpox virus.


Subject(s)
Disease Outbreaks , Vaccinia virus/isolation & purification , Vaccinia/epidemiology , Adolescent , Adult , Age Distribution , Aged , Animals , Base Sequence , Brazil/epidemiology , Chick Embryo , Child , Chlorocebus aethiops , Chorioallantoic Membrane , Female , Genes, Viral/genetics , Humans , Male , Microscopy, Electron , Middle Aged , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Vaccinia/diagnosis , Vaccinia virus/classification , Vaccinia virus/genetics , Vaccinia virus/ultrastructure , Vero Cells
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