ABSTRACT
Currently, industrial bioproducts are less competitive than chemically produced goods due to the shortcomings of conventional microbial hosts. Thus, is essential developing robust bacteria for improved cell tolerance to process-specific parameters. In this context, metagenomic approaches from extreme environments can provide useful biological parts to improve bacterial robustness. Here, in order to build genetic constructs that increase bacterial resistance to diverse stress conditions, we recovered novel protein-encoding sequences related to stress-resistance from metagenomic databases using an in silico approach based on Hidden-Markov-Model profiles. For this purpose, we used metagenomic shotgun sequencing data from microbial communities of extreme environments to identify genes encoding chaperones and other proteins that confer resistance to stress conditions. We identified and characterized 10 novel protein-encoding sequences related to the DNA-binding protein HU, the ATP-dependent protease ClpP, and the chaperone protein DnaJ. By expressing these genes in Escherichia coli under several stress conditions (including high temperature, acidity, oxidative and osmotic stress, and UV radiation), we identified five genes conferring resistance to at least two stress conditions when expressed in E. coli. Moreover, one of the identified HU coding-genes which was retrieved from an acidic soil metagenome increased E. coli tolerance to four different stress conditions, implying its suitability for the construction of a synthetic circuit directed to expand broad bacterial resistance.
ABSTRACT
Diferentes estratégias para divulgação de uma nova ferramenta de comunicação e interação em saúde, voltada para pessoas com deficiência, foram aplicadas no Facebook® com o objetivo de identificar quais estratégias de divulgação aplicadas ao Facebook® promoveram mais acessos à rede social D Eficiência e caracterizar seus usuários. Estudo quantitativo, descritivo e longitudinal, com dados extraídos do gerenciador de métricas do Facebook®. A divulgação com busca ativa foi superior a passiva, sendo responsável por 91,4% (n=4.519) dos acessos, via celular (71%) por mulheres (81%). Publicações com hiperlink e imagens foram mais engajadoras. O Facebook® mostrou-se como uma ferramenta eficaz por atingir números expressivos de pessoas, e deve ser considerado na divulgação de novas tecnologias. Para tal, é necessário criar conteúdos atrativos e busca ativa de usuários. Estudos complementares devem continuar a avaliação dessa estratégia, com análise da divulgação orgânica e divulgação com impulsionamento pago.
Different strategies for promoting a new health communication and interaction tool, aimed at persons with disabilities, were applied on Facebook® in order to identify which promotion strategies promoted hits to the D Eficiência social network, and to characterize its users. Quantitative, descriptive and longitudinal study, with data extracted from the Facebook® metrics manager. Active search promotion was higher than passive promotion, accounting for 91.4% (n=4,519) of hits, via cell phone (71%) by women (81%). Hyperlinked publications and images were more engaging. Facebook® proved to be an effective tool for reaching significant numbers of people and should be considered in the promotion of new technologies. For this, it is necessary to create attractive content and conduct an active search for users. Complementary studies should continue to evaluate this strategy, with analysis of organic promotion and paid promotion.
