ABSTRACT
RESUMO: Abordamos, no presente trabalho, a urgência subjetiva na adolescência, tendo como orientação a prática da psicanálise aplicada ao campo da saúde mental. Situamos a urgência subjetiva e apontamos impasses clínicos relativos ao seu manejo, relacionando-os ao modo como o mal-estar se apresenta na atualidade. A partir de fragmentos clínicos, discutimos a abordagem clínico-institucional, pontuamos a importância da escuta do detalhe e ressaltamos o lugar do analista como parceiro, ajudando a construir uma alteridade que dê suporte à travessia da adolescência.
ABSTRACT: We approach, in the present work, the subjective urgency in adolescence, having as orientation the practice of psychoanalysis applied to the field of mental health. We situate the subjective urgency and point out clinical impasses related to its management, relating them to the way in which the discontent presents itself today. From clinical fragments, we discuss the clinical-institutional approach, point out the importance of listening to the detail and emphasize the analyst's place as a partner, helping to build an alterity that supports the crossing of adolescence.
Subject(s)
Psychoanalysis , Mental Health , AdolescentABSTRACT
The present study demonstrated the potential effects of diethylcarbamazine (DEC) on monocrotaline (MCT)-induced pulmonary hypertension. MCT solution (600mg/kg) was administered once per week, and 50mg/kg body weight of DEC for 28days. Three C57Bl/6 male mice groups (n=10) were studied: Control; MCT28, and MCT28/DEC. Echocardiography analysis was performed and lung tissues were collected for light microscopy (hematoxylin-eosin and Masson's trichrome staining), immunohistochemistry (αSMA, FADD, caspase 8, caspase 3, BAX, BCL2, cytochrome C and caspase 9) western blot (FADD, caspase 8, caspase 3, BAX, BCL2, cytochrome C and caspase 9) and qRt-PCR (COL-1α and αSMA). Echocardiography analysis demonstrated an increase in the pulmonary arterial blood flow gradient and velocity in the systole and RV area in the MCT28 group, while treatment with DEC resulted in a significant reduction in these parameters. Deposition of collagen fibers and αSMA staining around the pulmonary arteries was evident in the MCT28 group, while treatment with DEC reduced both. Western blot analysis revealed a decrease in BMPR2 in the MCT28 group, in contrast DEC treatment resulted in a significant increase in the level of BMPR2. DEC also significantly reduced the level of VEGF compared to the MCT28 group. Apoptosis extrinsic and intrinsic pathway markers were reduced in the MCT28 group. After treatment with DEC these levels returned to baseline. The results of this study indicate that DEC attenuates PH in an experimental monocrotaline-induced model by inhibiting a series of markers involved in cell proliferation/death.
Subject(s)
Diethylcarbamazine/therapeutic use , Hypertension, Pulmonary/drug therapy , Actins/genetics , Animals , Apoptosis/drug effects , Apoptosis Regulatory Proteins/metabolism , Bone Morphogenetic Protein Receptors, Type II/metabolism , Collagen Type I/genetics , Diethylcarbamazine/pharmacology , Hypertension, Pulmonary/chemically induced , Hypertension, Pulmonary/pathology , Hypertension, Pulmonary/physiopathology , Hypertrophy, Right Ventricular/chemically induced , Hypertrophy, Right Ventricular/drug therapy , Hypertrophy, Right Ventricular/pathology , Hypertrophy, Right Ventricular/physiopathology , Lung/drug effects , Lung/metabolism , Lung/pathology , Lung/physiopathology , Male , Mice, Inbred C57BL , Monocrotaline , Pulmonary Artery/drug effects , Pulmonary Artery/pathology , Ventricular Function, Left/drug effectsABSTRACT
BACKGROUND: Hepatic myofibroblasts are relevant for pathogenesis of S. mansoni infection. In normal liver, these perisinusoidal cells are quiescent, express the lipocyte phenotype, and are located in the Disse's space, being the major site of vitamin A storage. When activated, they convert to myofibroblasts and contribute to granulomatous and diffuse liver fibrosis. In the present work, we observed that myofibroblasts obtained from granulomatous periovular inflammatory reactions in schistosome-infected mice (GR-MF) produce in vitro immunomodulatory cytokines for eosinophil activation: IL-5 and eotaxin. METHODS AND RESULTS: The secretory activity of GR-MF was detected after TGF-ß and IL-13 stimulation using 2D and 3D cell culture systems. In a mixed co-culture system using GR-MF with hematopoietic bone marrow cells from infected mice, we observed eosinophil survival that was dependent upon IL-5 and eotaxin, since antibodies against this cytokines decreased eosinophil population, as measured by eosinophil peroxidase activity. CONCLUSION: These results indicate that GR-MF may contribute to maintenance of local eosinophilia in schistosomal hepatic granulomas, and can function as immunoregulatory cells, besides their role in production of fibrosis.
