Ex-vivo evaluation of the intrapulpal temperature variation and fracture strength in teeth subjected to different external bleaching protocols.

This study evaluated the influence of bleaching protocols on intrapulpal temperature and fracture strength of the bleached teeth. Ninety maxillary incisors were assigned to 9 groups (n=10): G1: 35% carbamide peroxide (35% CP), G2: 38% hydrogen peroxide (38% HP), G3: halogen light, G4: LED-laser, G5: 35% CP + halogen light, G6: 38% HP + halogen light, G7: 35% CP + LED-laser, G8: 38% HP + LED-laser, and G9: no treatment (control). Pulp space was widened, a thermocouple was introduced through the apex up to the pulp chamber and the temperature was recorded (°C). The fracture strength (kN) was determined using an Instron machine. Data were analyzed by ANOVA and Tukey's test (p<0.05). The halogen light alone (1.10 ± 0.24) (G3) and associated with 38% HP (1.15 ± 0.30) (G6) produced the highest temperature increase, statistically different (p<0.05) from the other groups. G1 (0.15 ± 0.06) and G6 (0.19 ± 0.07) produced the lowest values, statistically different (p<0.05) from G5 (0.65 ± 0.49). The bleaching protocols increased the temperature, but no increment exceeded the critical value of 5.6°C. Neither 35% CP nor 38% HP or the light sources alone altered the fracture strength of the teeth.

Ex-vivo evaluation of the intrapulpal temperature variation and fracture strength in teeth subjected to different external bleaching protocols

This study evaluated the influence of bleaching protocols on intrapulpal temperature and fracture strength of the bleached teeth. Ninety maxillary incisors were assigned to 9 groups (n=10): G1: 35 percent carbamide peroxide (35 percent CP), G2: 38 percent hydrogen peroxide (38 percent HP), G3: halogen light, G4: LED-laser, G5: 35 percent CP + halogen light, G6: 38 percent HP + halogen light, G7: 35 percent CP + LED-laser, G8: 38 percent HP + LED-laser, and G9: no treatment (control). Pulp space was widened, a thermocouple was introduced through the apex up to the pulp chamber and the temperature was recorded (°C). The fracture strength (kN) was determined using an Instron machine. Data were analyzed by ANOVA and Tukey's test (p<0.05). The halogen light alone (1.10 ± 0.24) (G3) and associated with 38 percent HP (1.15 ± 0.30) (G6) produced the highest temperature increase, statistically different (p<0.05) from the other groups. G1 (0.15 ± 0.06) and G6 (0.19 ± 0.07) produced the lowest values, statistically different (p<0.05) from G5 (0.65 ± 0.49). The bleaching protocols increased the temperature, but no increment exceeded the critical value of 5.6°C. Neither 35 percent CP nor 38 percent HP or the light sources alone altered the fracture strength of the teeth.

Este estudo avaliou a influência de protocolos de clareamento na temperatura intrapulpar e resistência à fratura de dentes clareados. Noventa incisivos superiores foram divididos em 9 grupos (n=10): G1: peróxido de carbamida a 35 por cento (PC 35 por cento), G2: peróxido de hidrogênio a 38 por cento (PH 38 por cento), G3: luz halógena, G4: LED-laser, G5: PC 35 por cento + luz halógena, G6: PH 38 por cento + luz halógena, G7: PC 35 por cento + LED-laser, G8: PH 38 por cento + LED-laser e G9: sem tratamento (controle). O canal radicular foi alargado, um termopar foi introduzido no ápice até a câmara pulpar e a temperatura foi calculada (°C). A resistência à fratura (kN) foi determinada em máquina Instron. Os dados foram analisados por análise de variância e teste de Tukey (p>0,05). A luz halógena utilizada isoladamente (1,10 ± 0,24) (G3) e associada ao PH 38 por cento (1,15 ± 0,30) (G6) proporcionaram os maiores valores de temperatura, sendo estatisticamente diferentes dos demais grupos (p<0,05). O G1 (0,15 ± 0,06) e o G6 (0,19 ± 0,07) produziram os menores valores de resistência à fratura, sendo estatisticamente diferentes (p<0,05) do G5 (0,65 ± 0,49). Os protocolos de clareamento aumentaram a temperatura, mas nenhum incremento excedeu o valor crítico de 5,6oC. O PC 35 por cento, PH 38 por cento e as fontes de luz utilizadas isoladamente não alteraram a resistência à fratura dos dentes.

Cleaning of flattened root canals with different irrigating solutions and nickel-titanium rotary instrumentation.

The aim of this study was to evaluate the cleaning capacity of Profile .04 files combined with different irrigating solutions in flattened root canals using histological, morphometrical and SEM analyses. Eighty human mandibular incisors were prepared with Profile .04 instruments and randomly divided into 4 groups according to the irrigating solutions used (n=20): G1: distilled water (control); G2: 1% sodium hypochlorite (NaOCl); G3: 1% NaOCl alternated with 17% EDTA, and G4: 1% NaOCl with RCPrep cream. Ten teeth of each group were evaluated with an optical microscope to determine the percentage of root canal debris. The remaining teeth were evaluated under scanning electron microscopy (SEM). Data were analyzed statistically by ANOVA and Tukey's test (alpha=0.01).There was a significant difference (p<0.001) among the groups regarding the percentage of debris left in the canals (distilled water: 18.82 +/- 5.55; 1% NaOCl: 6.29 +/- 5.55; 1% NaOCl + 17% EDTA: 12.47 +/- 6.92; 1% NaOCl + RCPrep: 7.82 +/- 1.91). The SEM analysis showed the best results for 1% NaOCl + 17% EDTA on smear layer removal. It may be concluded that the combination of Profile .04 rotary instrumentation and the tested solutions was not able to totally remove debris and smear layer from flattened root canals.

Morphological alterations of radicular dentine pretreated with different irrigating solutions and irradiated with 980-nm diode laser.

BACKGROUND: The topographical features of intraradicular dentine pretreated with sodium hypochlorite (NaOCl) or ethylenediamine tetraacetic acid (EDTA) followed by diode laser irradiation have not yet been determined. PURPOSE: To evaluate the alterations of dentine irradiated with 980-nm diode laser at different parameters after the surface treatment with NaOCl and EDTA. STUDY DESIGN: Roots of 60 canines were biomechanically prepared and irrigated with NaOCl or EDTA. Groups were divided according to the laser parameters: 1.5 W/CW; 1.5 W/100 Hz; 3.0 W/CW; 3.0 W/100 Hz and no irradiation (control). The roots were splited longitudinally and analyzed by scanning electron microscopy (SEM) in a quali-quatitative way. The scores were submitted to two-way Kruskal-Wallis and Dunn's tests. RESULTS: The statistical analysis demonstrated that the specimens treated only with NaOCl or EDTA (control groups) were statistically different (P < 0.05) from the laser-irradiated specimens, regardless of the parameter setting. The specimens treated with NaOCl showed a laser-modified surface with smear layer, fissures, and no visible tubules. Those treated with EDTA and irradiated by laser presented absence of smear layer, tubules partially exposed and melting areas. CONCLUSIONS: The tested parameters of 980-nm diode laser promoted similar alterations on dentine morphology, dependent to the type of surface pretreatment.

Cleaning of flattened root canals with different irrigating solutions and nickel-titanium rotary instrumentation

The aim of this study was to evaluate the cleaning capacity of Profile .04 files combined with different irrigating solutions in flattened root canals using histological, morphometrical and SEM analyses. Eighty human mandibular incisors were prepared with Profile .04 instruments and randomly divided into 4 groups according to the irrigating solutions used (n=20): G1: distilled water (control); G2: 1 percent sodium hypochlorite (NaOCl); G3: 1 percent NaOCl alternated with 17 percent EDTA, and G4: 1 percent NaOCl with RCPrep cream. Ten teeth of each group were evaluated with an optical microscope to determine the percentage of root canal debris. The remaining teeth were evaluated under scanning electron microscopy (SEM). Data were analyzed statistically by ANOVA and Tukey's test (á=0.01).There was a significant difference (p<0.001) among the groups regarding the percentage of debris left in the canals (distilled water: 18.82 ± 5.55; 1 percent NaOCl: 6.29 ± 5.55; 1 percent NaOCl + 17 percent EDTA: 12.47 ± 6.92; 1 percent NaOCl + RCPrep: 7.82 ± 1.91). The SEM analysis showed the best results for 1 percent NaOCl + 17 percent EDTA on smear layer removal. It may be concluded that the combination of Profile .04 rotary instrumentation and the tested solutions was not able to totally remove debris and smear layer from flattened root canals.

O objetivo deste estudo foi avaliar a capacidade de limpeza dos instrumentos Profile .04 associados a diferentes soluções irrigantes, em canais radiculares achatados, por meio de análises histológica, morfométrica e MEV. Oitenta incisivos inferiores humanos foram submetidos ao preparo biomecânico com instrumentos Profile .04 e aleatoriamente distribuídos em 4 grupos de acordo com as soluções irrigantes usadas (n=20): G1 - água destilada (controle); G2 - NaOCl 1 por cento; G3 - NaOCl 1 por cento alternado com EDTA 17 por cento e G4 - NaOCl 1 por cento com RCPrep creme. Dez dentes de cada grupo foram avaliados em microscopia óptica para determinação da porcentagem de debris do canal radicular. Os demais dentes foram avaliados em microscopia eletrônica de varredura. A análise estatística mostrou diferença significante (p<0,001) entre os grupos ao considerar a porcentagem de debris remanescente nos canais (água destilada: 18,82 ± 5,55; NaOCl: 6,29 ± 5,55; NaOCl + EDTA: 12,47 ± 6,92; NaOCl + RCPrep: 7,82 ± 1,91). A análise em MEV evidenciou melhores resultados na remoção da camada de smear com o NaOCl 1 por cento alternado com EDTA 17 por cento. Pode-se concluir que a associação entre a instrumentação rotatória com Profile .04 e as soluções testadas não foi capaz de remover totalmente debris e camada de smear de canais radiculares achatados.