Dietary carotenoids regulate astaxanthin content of copepods and modulate their susceptibility to UV light and copper toxicity.

High irradiation and the presence of xenobiotics favor the formation of reactive oxygen species in marine environments. Organisms have developed antioxidant defenses, including the accumulation of carotenoids that must be obtained from the diet. Astaxanthin is the main carotenoid in marine crustaceans where, among other functions, it scavenges free radicals thus protecting cell compounds against oxidation. Four diets with different carotenoid composition were used to culture the meiobenthic copepod Amphiascoides atopus to assess how its astaxanthin content modulates the response to prooxidant stressors. A. atopus had the highest astaxanthin content when the carotenoid was supplied as astaxanthin esters (i.e., Haematococcus meal). Exposure to short wavelength UV light elicited a 77% to 92% decrease of the astaxanthin content of the copepod depending on the culture diet. The LC(50) values of A. atopus exposed to copper were directly related to the initial astaxanthin content. The accumulation of carotenoids may ascribe competitive advantages to certain species in areas subjected to pollution events by attenuating the detrimental effects of metals on survival, and possibly development and fecundity. Conversely, the loss of certain dietary items rich in carotenoids may be responsible for the amplification of the effects of metal exposure in consumers.

Effects of phenanthrene- and metal-contaminated sediment on the feeding activity of the Harpacticoid Copepod, Schizopera knabeni.

The effects of sediments contaminated with sublethal concentrations of phenanthrene (PAH) and metals (Cd, Hg, Pb) were evaluated in relation to their influence on the feeding activity of a harpacticoid copepod, Schizopera knabeni. A metal mixture (at the ratio of 5Pb:3Cd:2Hg) and Cd alone reduced grazing rates of S. knabeni feeding on (14)C-labeled microalgae. Cadmium alone and Cd combined with phenanthrene significantly decreased grazing rates of S. knabeni at Cd concentrations above 49 mg kg(-1) dry sediment. No grazing was observed in 98, 106, or 157 mg kg(-1) dry sediment Cd alone or in sediment contaminated with phenanthrene (98 mg kg(-1) dry sediment) combined with Cd at these concentrations. Phenanthrene alone also caused a significant decrease (55%) in S. knabeni grazing rates. Feeding ceased above 344 mg kg(-1) dry sediment of the metal mixture alone and combined with phenanthrene. Results were consistent with an independent effect on feeding when Cd and phenanthrene were combined. When other metals were added (Pb and Hg) to the mixture, results were consistent with an additive influence on feeding rate. Because the underlying mechanisms of toxicity for metals and PAH are probably different, our observations suggest that reductions in grazing probably did not directly contribute to the lethal effects of phenanthrene or metals. The absence of interactive effects on feeding suggests that metal-PAH interactive effects on lethality have a different underlying mechanism and that reductions in grazing probably did not directly contribute to the lethality effects of phenanthrene or metals in S. knabeni.