Effect of Storage Time on Bond Strength Performance of Multimode Adhesives to Indirect Resin Composite and Lithium Disilicate Glass Ceramic.

PURPOSE: To investigate the bond strength performance of multimode adhesives (MMAs) to indirect resin composite and lithium disilicate glass ceramic after 24 hours or one year of water storage. METHODS AND MATERIALS: Thirty flat and polished plates of indirect resin composite (Epricord) and thirty lithium disilicate glass ceramic plates (IPS e.max Press) were prepared. Surfaces were pretreated using sandblasting (indirect resin composite) or hydrofluoric acid (glass-based ceramic). Specimens were bonded with one of two MMAs (Scotchbond Universal [SBU] or All-Bond Universal [ABU]) or ceramic primer and hydrophobic bonding (RelyX Ceramic Primer and Adper Scotchbond Multi-Purpose Bond) as a control (n=10). Resin cement cylinders (0.75 mm in diameter × 0.5 mm in height) were bonded to both substrate surfaces using the respective adhesives. After 24 hours or one year of water storage, bonding performance was measured by microshear bond strength (MSBS) testing. Results were analyzed using three-way ANOVA with Bonferroni post hoc tests (α=0.05). RESULTS: For indirect resin composite, significantly higher MSBS values were found for ABU after 24 hours (ABU > SBU = control); however, no significant difference among the adhesives was observed after one year (p>0.05). For glass-based ceramic, significantly different bond strengths were observed among the adhesives after 24 hours (control = ABU > SBU) and one year (control > SBU = ABU; p<0.05). CONCLUSIONS: Both MMAs tested can be considered effective alternatives for bonding to sandblasted indirect resin composite after aging, as they showed similar bond performance to that of the control group. However, separate bottles of silane bonding resin showed higher MSBS values and more durable bonding for etched glass-based ceramic.

Methods to assess the viability of cryopreserved Jatropha curcas L. seed germplasm / Métodos para avaliar a viabilidade de germoplasma semente criopreservado de Jatropha curcas L

ABSTRACT Jatropha curcas L. is a plant species with many potential applications, especially medicinal uses (hypoglycemic, anti-inflammatory, haemostatic, healing, anti-tumor). The objective of this study was to test germination in moist paper rolls for whole seeds and in vitro for excised embryonic axes, in an attempt to identify the best method to assess the quality of J. curcas seed germplasm, cryopreserved with different water contents. The experimental sample with a 6.2% moisture content (MC) was divided in subsamples which were hydrated and dehydrated for 0 (control), 4, 8, 11 and 24h. The initial germination percentages were 63% for whole seeds and 81% for excised embryonic axes. After exposure to liquid nitrogen (LN), germination percentages were 48% (whole seeds) and 57% (excised embryonic axes). There was no significant difference between germination percentages in embryonic excised from seeds subjected or not subjected to freezing, with different MC. In contrast, there was a reduction of the whole seed germination percentage when exposed to LN (contrast = 0.17, standard error = 0.04, t = 4.09, p = 0.001) and not for the hydration and dehydration treatments. The methodology based on in vitro cultures of the embryonic axis isolated from seeds stored in LN with distinct MC values was more efficient than the standard germination test to evaluate the viability of J. curcas seeds before and after LN storage.

RESUMO Jatropha curcas L., é uma espécie com várias aplicações potenciais, principalmente para usos medicinais (hipoglicemina, anti-inflamatório, homeostático, cicatrizante, antitumoral). O objetivo deste estudo foi testar a germinação em rolo de papel para sementes inteiras e in vitro para eixos embrionários excisados visando identificar o melhor método para avaliar a qualidade de germoplasma semente de J. curcas, criopreservado com diferentes teores de água. A amostra experimental com 6,2% de teor de água foi dividida em subamostras que foram hidratadas e desidratadas por 0 (controle), 4, 8, 11 e 24 h. Os percentuais de germinação inicial foram de 63% para sementes inteiras e de 81% para eixos embrionários excisados. Após exposição ao nitrogênio líquido (NL) os percentuais de germinação foram de 48% (sementes inteiras) e 57% (eixos embrionários). Não houve diferença significativa entre os percentuais de germinação de eixos embrionários excisados de sementes com diferentes umidades e submetidas ou não ao congelamento. Em contraste, houve redução de percentuais de germinação das sementes inteiras expostas ao NL (contraste = 0.17, erro padrão = 0.04, t = 4.09, p = 0.001), mas não aos tratamentos de hidratação e desidratação. A metodologia baseada em cultura de eixos embrionários in vitro isolados de sementes armazenadas em NL, com distintos teores de água foi mais eficiente que a germinação padrão para avaliar a viabilidade de sementes J. curcas antes e após a armazenamento em NL.

Rendimento, composição química e atividades antimicrobiana e antioxidante do óleo essencial de folhas de Campomanesia adamantium submetidas a diferentes métodos de secagem / Content, chemical composition and antimicrobial and antioxidant activities of the essential oil from leaves of Campomanesia adamantium submitted to different drying methods

RESUMO Campomanesia adamantium é muito utilizada na medicina popular no tratamento de hipertensão, reumatismo e diabetes. Objetivou-se com o presente trabalho avaliar o rendimento, a composição química, e as atividades antimicrobiana e antioxidante do óleo essencial de folhas de C. adamantium submetidas a diferentes métodos e tempos de secagem. O delineamento utilizado foi inteiramente casualizado, com oito tratamentos e três repetições. O óleo essencial foi extraído por hidrodestilação das folhas e analisado por CG-EM. O tipo de secagem das folhas de C. adamantium influenciou no teor e na composição química do óleo essencial. Os constituintes espatulenol, óxido cariofileno e germacreno-B apresentaram maior concentração na maioria das procedências avaliadas. O óleo essencial das folhas de C. adamantium apresentou moderada atividade antibacteriana em relação a todos os micro-organismos testados. A atividade antioxidante se deu empregando-se o método de redução do radical estável DPPH, sendo observada fraca atividade antioxidante por meio do ensaio utilizado.

ABSTRACT C. adamantiumis widely used in popular medicine for thetreatment of hypertension, rheumatism and diabetes. This study looked to evaluate the essential oil yield, the chemical composition and the antimicrobial and antioxidant activities of the essential oil from leaves of C. adamantium. A completely randomized design was used with eight treatments and three replications. The essential oil obtained by hydrodestillation of the leaves was analyzed by GC-MS. The results show that the drying type and the processing of the leaf influence the content and chemical composition of the essential oil. The compounds spathulenol, caryophyllene oxide and germacrene-B have higher concentrationsin mostorigins. The essentialoil from theleaves of C.adamantiumshowed moderate bacterial activityagainst all tested microorganisms. The antioxidant activity was determined by the methods of reduction of the DPPH stable radical. The antioxidant activity was weak in the test used.

Gastroprotective activity of α-terpineol in two experimental models of gastric ulcer in rats.

BACKGROUND AND THE PURPOSE OF THE STUDY: Several plant essential oils, as well as terpenes present in essential oils, have shown gastroprotective activity. The aim of the present work was to evaluate the gastroprotective activity of α-terpineol, a monoterpene alcohol which is present in essential oils of various plants. METHODS: The gastroprotective activity of α-terpineol was evaluated in rats by assessing the changes in ethanol and indomethacin-induced gastric ulcer scores and on gastric secretory volume and total acidity in pylorus-ligated rats. Alpha-terpineol was administrated orally at the doses of 10, 30, and 50 mg/kg one hour before administration of the ulcer inducing agents by the pylorus ligation procedure. The involvement of endogenous prostaglandins in the protective effect of α-terpineol in ethanol-induced gastric lesions test was assessed by administration of indomethacin (10 mg/kg, s.c.) 30 min before oral administration of α-terpineol at the dose of 50 mg/kg. RESULTS: α-terpineol presented gastroprotective activity against ethanol-induced ulcers at the doses of 10, 30, and 50 mg/kg. Epoxy-carvone at the dose of 10 mg/kg did not present gastroprotective activity against ulcer induced by indomethacin, but at the doses of 30 and 50 mg/kg it attenuated the gastric damages induced by this agent significantly. Pretreatment with indomethacin did not prevent the gastroprotective effect of α-terpineol on ethanol-induced ulcers. Alpha-terpineol also did not affect the gastric secretion in pylorus-ligated rats. MAJOR CONCLUSION: The results suggest that α-terpineol presents gastroprotective action which does not involve either an increase in the synthesis of endogenous prostaglandin or a decrease in the gastric acid secretion.

A inserção do nutricionista na Atenção Primária e na Saúde da Família - Juliana Paulo e Silva

Juliana abordou a Estratégia de Saúde da Família, priorizada pelo Ministério da Saúde para organizar a Atenção Primária, com o desafio de promover a reorientação das práticas e ações de saúde de forma integral e contínua. Outra iniciativa apontada por Juliana foi a criação dos Núcleos de Apoio à Saúde da Família (Nasf), com o objetivo de ampliar a abrangência e o escopo das ações da Atenção Básica, bem como sua resolubilidade, apoiando a inserção da estratégia de Saúde da Família na rede de serviços e o processo de territorialização e regionalização a partir da Atenção Básica. Por fim, a nutricionista citou os avanços da nutrição na Atenção Básica, entre os quais diretrizes alimentares oficiais estabelecidas, Rede de Nutrição no SUS consolidada e publicações na área de Nutrição na Atenção Primária à Saúde para orientar a ação. Arquivo disponível para audição e/ou download nos ícones ao lado.