Similarities of periodontal clinical and microbiological parameters in mother-child pairs.

The aim of this study was to verify the association between children and their mothers (N = 28) for periodontal clinical and microbiological measures. Periodontal clinical parameters (probing depth and bleeding on probing) were obtained from six reference teeth from each mother-child pair. In addition, subgingival plaque samples taken from the same reference teeth were collected and placed on the Perioscan test for the detection of Treponema denticola, Porphyromonas gingivalis and Bacteroides forsythus. There were statistically significant differences between children and mothers regarding probing depth; there was an overall tendency for mothers to exhibit average probing depths greater than their children. In contrast, if a child had a bleeding site, the respective site on the mother usually also bled on probing, implying that there was an association in terms of bleeding between the mother-child pairs. Similar findings were observed for the Perioscan test. It is concluded that the occurrence of bleeding and periodontal anaerobic infections (as determined by the Perioscan test) were similar in reference teeth of mother-child pairs. These data suggest that parents with periodontal disease may serve as a reservoir of periodontopathic organisms for their children.

Carotid-aortic and renal baroreceptors mediate the atrial natriuretic peptide release induced by blood volume expansion.

Our previous studies have shown that stimulation of the anteroventral third ventricle (AV3V) region of the brain increases atrial natriuretic peptide (ANP) release, whereas lesions of the AV3V region or median eminence of the tuber cinereum block the release of ANP caused by blood volume expansion. These results suggest that participation of the central nervous system is critical to this response. The role of baroreceptors in the response was evaluated in the current research by studying the response of plasma ANP to blood volume expansion induced by intravenous injection of hypertonic saline solution (0.3 M NaCl, 2 ml/100 g of body weight, over 1 min) in conscious, freely moving male rats. Plasma samples were assayed for ANP by radioimmunoassay. In sham-operated rats, blood volume expansion induced a rapid increase in plasma ANP: the concentration peaked at 5 min and remained elevated at 15 min after saline injection. One week after deafferentation of the carotid-aortic baroreceptors, basal plasma ANP concentrations were highly significantly decreased on comparison with values of sham-operated rats; plasma ANP levels 5 min after blood volume expansion in the deafferented rats were greatly reduced. Unilateral right vagotomy reduced resting levels of plasma ANP but not the response to blood volume expansion; resting concentrations of plasma ANP and responses to expansion were normal in bilaterally vagotomized rats. In rats that had undergone renal deafferentation, resting levels of ANP were normal but the response to blood volume expansion was significantly suppressed. The evidence indicates that afferent impulses via the right vagus nerve may be important under basal conditions, but they are not required for the ANP release induced by blood volume expansion. In contrast, baroreceptor impulses from the carotid-aortic sinus regions and the kidney are important pathways involved in the neuroendocrine control of ANP release. The evidence from these experiments and our previous stimulation and lesion studies indicates that the ANP release in response to volume expansion is mediated by afferent baroreceptor input to the AV3V region, which mediates the increased ANP release via activation of the hypothalamic ANP neuronal system.

Renal sodium handling after noradrenergic stimulation of the lateral hypothalamic area in rats.

1. The participation of special nephron segments in the renal control of sodium handling after adrenergic stimulation was investigated by determining lithium clearance in groups of 5-12 male Wistar rats (230-300 g) microinjected with noradrenaline into the lateral hypothalamic area (LHA). 2. Microinjection of noradrenaline (12.5 to 100.0 nmol/microliters) into the LHA promoted a significant decrease in proximal sodium reabsorption (controls, 86.5 +/- 1.3; 12.5, 81.4 +/- 2.0; 25.0, 72.6 +/- 2.4; 50.0, 75.4 +/- 1.8 and 100.0, 77.2 +/- 1.7%) and a dose-related increase in distal sodium reabsorption (control, 13.4 +/- 1.6; 12.5, 18.4 +/- 1,25.0, 26.9 +/- 2.9; 50.0, 24.1 +/- 2.7; 100.0, 22.1 +/- 1.9%) with no significant changes in creatinine clearance. Fractional sodium reabsorption after different noradrenaline concentrations was significantly reduced in the proximal nephron sites up to the concentration of 25.0 nmol/microliter. Beyond this concentration, a smaller but progressive increase in fractional sodium reabsorption was observed in the post-proximal segment. 3. These findings suggest an effective participation of proximal and post-proximal nephrons in natriuresis after lateral hypothalamic noradrenergic stimulation.

Renal sodium handling after noradrenergic stimulation of the lateral hypothalamic area in rats

The participation of specific of special nephron segments in the renal control of sodium handling after adrenergic stimulation was investigated by determining lithium clearance in groups of 5-12 male Wistar rats (230-300 g) microinjected with noradrenaline into the lateral hypothalamic area (LHA). Microinjection of noradrenaline (12.5 to 100.0 nmol/ul) into the LHA promoted a significant decrease in proximal sodium reabsorption (control, 86.5 ñ 1.3; 12.5,81.4 ñ 2.4; 50.0, 75.4 ñ 1.8 and 100.0,77.2 ñ 1.7%) and a dose-related increase in distal sodium reabsorption (control, 13.4 ñ 1.6; 12.5, 18.4 ñ 1.25.0,26.9 ñ 2.9; 50.0,24.1 ñ 2.7; 100.0,22.1 ñ 1.9%) with no significannt changes inm creatinine clearance. Fractional sodium reabsorption after different noradrenaline concentrations was significantly reduced in the proximal nephron sites up to the concentration of 25.0 nmol/ul. Beyond this concentration, a smaller but progressive increase in fraqctional sodium reabsorption was observed in the post-proximal segment. These findings suggest an effective participation of proximal and post-proximal nephrons in natriuresis after lateral hypothalamic noradrenergic stimulation

Renal water handling evaluated by lithium clearance following adrenergic and cholinergic stimulation of the lateral hypothalamic area.

Male Wistar rats weighing 230-300 g were used to characterize the participation of adrenergic and cholinergic receptors of the lateral hypothalamic area (LHA) in the control of renal water excretion. Since stimulation of adrenergic or cholinergic receptors has no effect on glomerular filtration rate, the antidiuresis and significant delay in urinary flow observed after lateral hypothalamic stimulation with carbachol (CCh) (0.036 +/- 0.005 to 0.019 +/- 0.003 microliters min-1 100 g body weight-1) and noradrenaline (Nad) (0.024 +/- 0.005 to 0.025 +/- 0.004 microliters min-1 100 g body weight-1) are secondary to an increase in distal tubular fluid reabsorption (DFR). Data are reported as means +/- SEM for ten rats in each group. Tubular water handling measured by lithium clearance demonstrated that LHA simulation with CCh (2.8 nmol in 1 microliter) and Nad (30.0 nmol in 1 microliter) leads to a significant reduction in proximal water reabsorption (CCh, 93.3 +/- 2.6 to 85.4 +/- 1.4%; Nad, 92.7 +/- 0.9 to 88.6 +/- 1.3%), with a simultaneous and significant increase in fluid reabsorption along the post-proximal nephron segments when compared to control (CNa) (CCh, 6.7 +/- 0.7 to 14.5 +/- 1.1%; Nad, 8.2 +/- 0.8 to 11.4 +/- 1.6%). These effects are blocked by muscarinic (atropine, 5 nmol in 1 microliter) and alpha-1 adrenoceptors (prazosin, 4 nmol in 1 microliter) antagonists. The results indicate the effective participation of the post-proximal nephron in the antidiuresis occurring after cholinergic and adrenergic LHA stimulation.

Renal water handling evaluated by lithium clearance following adrenergic and cholinergic stimulation of the lateral hypothalamic area

Male Wistar rats weighing 230-3--g were used to characterize the participation of adrenerg and cholinergic receptors of the lateral hypothalamic area (LHA) in the control of renal water excretion. Since stimulation of adrenergic or cholinergic receptors has no effect on glomerular filtration rate, the antidiuresis and significant delay in urinary flow observed after lateral hypothalamic stimulation with carbachol (CCh) (0.036 ñ 0.005 to o.019 ñ 0.003 µlmin-1 100g body weight-1) and noradrenaline (Nad) (0.024 ñ 0.005 to 0.025 ñ 0.004 µl min-1 100g body weight-1) are secondary to an increase in distal tubular fluid reabsorption (DFR). Data are reported as means ñ SEM for ten rats each group. Tubular water measured by lithim clearance demonstrated that LHA stimulation with CCh (2.8 nmol in 1 µl) and Nad (30.0 nmol in µl) leads to a significant reduction in proximal water reabsorption with CCh, 93.3 ñ 2.6 to 85.4 ñ 1.4%; Nad, 92.7 ñ0.9 to 88.6 ñ 1.3%), with a simultaneous and significant incrase in fluid reabsorption along the post-proximal nephrom segments when compared to control (CNa) (CCh, 6.7 ñ 0.7 to 14.5 ñ 1.1%; Nad 8.2 ñ 0.8 to 11.4 ñ 1.6%) These effects are blocked by muscarinic (atropine, 5 nmol in 1 µl) and alpha-1 adrenoceptors (prazosin, 4 nmol in 1 µl) antagonists. The results indicate the effective participation of the post-proximal nephron in the antidiuresis occurring after cholinergic and adrenergic LHA stimulation

[Experimental hypervitaminosis A in the rat. 14. Morphological and morphometric study of changes in the esophageal epithelium]. / Hipervitaminose A experimental no rato. XIV. Estudo morfológico e morfométrico das alterações no epitélio do esôfago.

Male rats were subjected to hypervitamin A treatment by daily intraperitoneal injections of 150 UI Arovit per g body weight. Another group of rats were injected intraperitoneally with saline solution for ten days and were used as controls. The results indicated that: 1) the body weight of animals treated with excess vitamin A was decreased; 2) histological analysis of the esophageal epithelium in treated animals showed increased thickness, characterizing a picture of hyperplasia and hypertrophy. On the basis of the results obtained, we suggest that excess vitamin A may act on the esophageal epithelium by direct action on the epithelial structures, stimulating and increase of the mitosis, and on the epithelium (mediated by the adrenals), resulting in increased thickness due to the concentration of a larger number of more immature cells. These alterations were more evident in the lower esophageal third.

[Oral hygiene in university students]. / Higiene bucal em universitários.

The family income of students entering the University suggests that they should be aware of basic rules of oral hygiene. Thus, oral hygiene was evaluated in a study conducted on 336 1st-year students enrolled in the Pharmacy and Dentistry Course of Ribeirão Preto, USP. The results showed that 42% of the students consult a dentist twice a year; the frequency of tooth brushing was four times a day, with no difference between the use of soft (48%) and medium (49%) brushes, and brushing time was 1 to 5 min. The students use dental floss (87%) and rinse their mouth with Cepacol (37%), and females brush their tongue at higher frequency (70%). Fifty-two percent of the students reported the occurrence of gingival bleeding when they brush their teeth.

[Factors that alter taste perception]. / Fatores que alteram a percepção gustativa.

Dysfunction of taste perception is a significant problem for many individuals. Taste anomalies may affect health not only by directly affecting liquid and solid food intake, but also by creating a state of depression due to the loss of an important source of pleasure. Many factors alter taste perception, such as lesions of the oral mucosa, cigarette smoking, radiation, chemotherapy, renal disease, hepatitis, leprosy, hormones, nutrition, use of dentures, medications, and aging. Gum or ice chewing may temporarily help loss of taste. Patients should be encouraged to chew their food thoroughly, alternating the sides of the mouth, or alternating different foods. Unfortunately, in many cases there is no cure for this alteration, and patience is then the only possibility.

Participation of opiate pathways in the lateral hypothalamic area in the control of renal electrolyte and water excretion.

Injection of Met-enkephalin (0.05, 0.25 and 0.50 micrograms in 1 microliter) solutions into the lateral hypothalamic area (LHA) of unrestrained and unanesthetized rats caused a significant decrease of sodium (0.39 +/- 0.13 to 0.07 +/- 0.01, P less than 0.05) and potassium (0.61 +/- 0.17 to 0.21 +/- 0.04, P less than 0.05) excretion. When the blocking agent naloxone (0.20 micrograms in 1 microliter) was injected alone, a significant increase of sodium (0.34 +/- 0.04 to 0.96 +/- 0.28, P less than 0.05) and potassium (0.76 +/- 0.13 to 1.72 +/- 0.30, P less than 0.05) excretion was observed. However, a dose-response relationship was not observed. However, when in another experiment naloxone was injected before Met-enkephalin into the same area, reversal of the effect of naloxone occurred, with decreased sodium and potassium excretion. We conclude that the enkephalinergic pathway of the LHA when stimulated with Met-enkephalin plays an inhibitory role in the control of sodium and potassium excretion.

Effects of injection of selective adrenergic receptor antagonists into the lateral hypothalamic area on renal function.

Studies were undertaken to characterize the participation of specific alpha-1,alpha-2 and beta adrenoceptors of the lateral hypothalamic area (LHA) in the urinary excretion of sodium and potassium. Alpha-1 and alpha-2 LHA receptors were shown to participate in the regulation of renal sodium and potassium excretion. The effects of noradrenaline microinjection (30 nmol in 1 microliter) into the LHA on urinary sodium excretion (UNaV) are blocked by previous injection of the alpha-1 antagonist prazosin (4 nmol in 1 microliter) from 3.22 +/- 0.25 to 0.59 +/- 0.04 microEq min-1 100 g body weight-1. Pre-injection of yohimbine, an alpha-2 antagonist (4 nmol in 1 microliter), synergistically potentiated the action of noradrenaline on UNaV (3.22 +/- 0.25 to 4.02 +/- 0.27 microEq min-1 100 g body weight-1) and on urinary potassium excretion (UKV) (0.70 +/- 0.08 to 1.15 +/- 0.12 microEq min-1 100 g body weight-1). The beta-adrenergic blockers metoprolol (100 nmol in 1 microliter) and propranolol (100 nmol in 1 microliter) had no synergistic or antagonistic action on the sodium excretion fraction, suggesting that neither of these receptors is present in LHA. Our results indicate that natriuresis occurs even in the absence of changes in glomerular filtration rate and demonstrate an inhibitory natriuretic effect of an alpha-1 blocker (prazosin) injected into the LHA before adrenaline, while an alpha-2 antagonist (yohimbine) yielded a potentiating effect.

[Restorative dentistry in a community center. 10-year report]. / Dentística restauradora em centro comunitário--Relato de 10 anos.

It is reported the results of voluntary odontological assistance in the Community Center of the Methodist Church of Vila Virginia. In a period of ten years the relation patient/dentistry student was an average of 23.08. Of the procedures, 69.9 per cent were amalgam restaurations and 30.1 per cent with silicate cement or resin. This is an important extracurricular activity for the student, in the sense that it helps improve his techniques. On the other hand, although restricted in time, this work has been important for the oral health of the community. Uniterms: voluntary extracurricular activity; amalgam, silicate or resin restaurations.

Inhibition of gastric acid secretion by cholinergic stimulation of the lateral hypothalamic area.

Cholinergic stimulation of the lateral hypothalamic area with carbachol (1 microgram in 1 microliter) markedly inhibited gastric acid secretion in the anesthetized rat. Inhibition was blocked by prior micro-injection of atropine (4 micrograms/microliters) into the same brain area and was accompanied by an increased sodium content in the stomach. Muscarinic receptor mediated cholinergic inhibitory influence of the hypothalamus on gastric acid secretion is suggested by these results.

Inhibition of renal electrolyte excretion by gabaergic pathways of the lateral hypothalamic area.

Injection of gamma amino butyric acid (GABA) into the lateral hypothalamic area of unrestrained conscious rats caused a decrease in renal electrolyte excretion with an increase in urinary flow. When picrotoxin, a specific inhibitor of gabaergic pathways, was administered, a significant increase in renal water and electrolyte excretion occurred. The effect of simultaneous injection of picrotoxin and GABA into the same site indicated that picrotoxin was less potent in reversing the effect induced by GABA than GABA was in reversing the effect of picrotoxin. We conclude that GABA acts directly on the neuronal mechanisms involved in the control of water and electrolyte excretion, perhaps by exerting a tonic inhibitory action on renal electrolyte excretion.

Inhibition of renal electrolyte excretion by gabaergic pathways of the lateral hypothalamic area

Injection of gamma butyric acid (GABA) into the lateral hypothalamic area of unrestrained conscious rats caused a decrease in renal electrolyte excretion with an increase in urinary flow. When picrotoxin, a specific inhibito of gabaergic pathways, was administered, a significant increase in renal water and electrolyte excretion occured. The effect of simultaneous injection of pidrotoxin and GABA into the same site indicate that picrotoxin was less potent in reversing tyhe effect induceb by GABA than GABA was in reversing the effect of picrotoxin. We conclude that GABA acts directly on the neuronal mechanisms involved in the control of water and elecltrolyte excretion, perhaps by exerting a tonic inhibitory action on renal electrolyte excretion

Effects of injection of selective adrenergic receptor antagonists into the lateral hypothalamic area on renal function

Studies were underlaken to characterize the participation of specific alpha-1, alpha-2 and beta adrenoceptors of the lateral hypothalamic area *LHA) in the urinary excretion of sodium and potassium. Alpha-1 and alpha-2 IHA receptors were shown to participate in the regulation of renal sodium and potassium excretion. the effects of noradrenaline microinjection (30 nmol in 1 micronl) into the LHA on urinary sodium excretion (UNaV) are blocked by previous injection of the alpha-1 antagonist prazosin (4 nmol in 1 micronl) from 3.22 ñ 0.25 to 0.59 ñ 0.04 micronEq min-1 100g body weigh-1. Pre-injection of yohimbine, an alpha-2 antaghonist (4 nmol in 1 micronl), synergistically potentiated the action of noradrenaline on UNaV (3.22 ñ 0.25 to 4.02 ñ0.27 micronEq min-1 100g body weight-1) and on urinary potassium excretion (UKV) (0.70 ñ 0.08 to 1.15 ñ 0.12 micronEq min-1g body weight-1). The beta-adrenergic blockers metoprolol (100 nmol in 1 micronl) and propranolo (100 nmol in 1 micronl) had no sunergistic or antagonistic action on the sodium excretion fraction, suggesting that neither of these recptors in present in the LHA. Our results indicate that natriuresis occurs even in the absence of changes in glomerular filtration rate and demonstrate an inhibitory natriuretic effect of an alpha-1 blocker (prazosin) injected into the LHA before adrenaline, wile an alpha-2 antagonsit (yohimbine) yielded a potentiating effect (AU)ñ

Inhibition of gastric acid secretion by cholinergic stimulation of the lateral hypopthalamic area

Cholinergic stimulation of the lateral hypothalamic area with carbachol (1 microng in 1 micronl) markedly inhibited gastric acid secretion in the anesthetized rat. Inhibition was bloced by prior microinjection of atropine (4 microng/micronl) into the same brain area and was accompanied by an increased soldium content in the stomach. Muscarinic receptor mediated cholinergic inhibitory influence of the hypothalamus on gastric acid secretion is suggested by these results

Changes in urinary electrolytes excretion after injections of ionic solutions into lateral hypothalamic area of rats.

Rats submitted to a chronically implanted cannula into lateral hypothalamic area (LHA) were utilized to study the effect of ionic stimulation of KCl, LiCl and CaCl2, sodium free solutions, on renal water and electrolyte excretion. In a general way decreases in natriuresis and kaliuresis following the injections of solutions into LHA were observed. Furthermore, significant increases in diuresis were noted after the injections of the 0.30 M solutions.

Aspectos morfológicos e funcionais renais no desenvolvimento da hipertensäo arterial em ratos espontaneamente hipertensos / Renal morphologic and functional aspects in the development of blood hypertension in spontaneously hypertensive rats

Foram observadas alteraçöes estruturais nos rins de ratos espontaneamente hipertensos (SHR) que antecedem o estado hipertensivo e as alteraçöes hemodinâmicas do nefro. Estes achados podem representar defeito genético estrutural ou estarem associados a hiperatividade autonômica simpática prévia, já observada nos animais jovens pré-hipertensos. Ainda, essas alteraçöes poderiam desempenhar certo papel no desenvolvimento e na manutençäo dos níveis tensionais elevados nesta linhagem de ratos. Os resultados do presente trabalho mostram alteraçöes funcionais peculiares no SHR e confirmam aquelas observadas em animais normotensos adultos após a desnervaçäo renal bilateral

[Morphometric study of changes in the submandibular gland of aging rats. 2. Ducts]. / Estudo morfométrico das alterações da glândula submandibular do rato, determinadas pelo envelhecimento. II. Ductos.

A decrease in rat submandibular gland parenchyma could be observed with increasing age, consequent to an apparent increase in the proportion of intralobular ducts. Signs of acinar atrophy were noted with nuclei of smaller volume. A great variety of nuclear sizes and shapes was also observed. The blood vessels were smaller, and the connective tissue was increased. These histological data were confirmed in part by application of the technique of Chalkley. The granulous duct cell height is smaller in old animals.