ABSTRACT
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Subject(s)
Child , Adult , Aged , Male , Female , Humans , Voice Disorders , Thrombolytic Therapy , Myocardial Infarction , Multiple Organ Failure , Necrosis , Terminology , Tissue Plasminogen Activator , Anticonvulsants , Cerebral Hemorrhage , Acute Disease , Acetates , Magnetic Resonance Imaging , Fibrinolytic Agents , Influenza A virus , Brain Diseases , Influenza, HumanABSTRACT
The production of sorbitol and gluconic acid by isolated glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis has been studied in a convective, 100-mL loop reactor with tangential ultrafiltration. Using a dilution rate of 0.04/h and 5 kU/L GFOR, substrate conversion (3 M sugar) in a single stage was >85%, and productivities of 126 g sorbitol/(L x d) were obtained. At a constant recycle rate (3/min) and a membrane area of 50 cm2, the dilution rates (and thus productivities) were however limited by a more than 30-fold reduction of the permeate flow in the presence of high sugar and protein concentrations (5 g/L). Protein was added, together with 10 mM dithiothreitol, to improve the stability of GFOR during substrate turnover and crossflow filtration, thus leading to a stable operation of the enzyme reactor for at least 5 d.
ABSTRACT
This article is a brief description related to maternal and child health problems in Mexico, with an introduction of Latin-American scope of the problem. The author mentions also the up-to-date systems for the analysis of these health problems, like the indicators based on life-level instead of infant mortality or maternal mortality and others; however the author chooses to use the traditional indicators in his article.