ABSTRACT
New facilities such as the National Ignition Facility and the Linac Coherent Light Source have pushed the frontiers of high energy-density matter. These facilities offer unprecedented opportunities for exploring extreme states of matter, ranging from cryogenic solid-state systems to hot, dense plasmas, with applications to inertial-confinement fusion and astrophysics. However, significant gaps in our understanding of material properties in these rapidly evolving systems still persist. In particular, non-equilibrium transport properties of strongly-coupled Coulomb systems remain an open question. Here, we study ion-ion temperature relaxation in a binary mixture, exploiting a recently-developed dual-species ultracold neutral plasma. We compare measured relaxation rates with atomistic simulations and a range of popular theories. Our work validates the assumptions and capabilities of the simulations and invalidates theoretical models in this regime. This work illustrates an approach for precision determinations of detailed material properties in Coulomb mixtures across a wide range of conditions.
ABSTRACT
The antimicrobial drug 1-methyl-2-nitro-5-vinylimidazole (MEV) preferentially blocked DNA synthesis, was mutagenic and induced coliphage lambda in Escherichia coli. The antibacterial effects of MEV are the consequences of repairable damage to DNA, as shown by hypersensitivity of recA and uvr strains to MEV and related drugs, stimulation by MEV of DNA turnover which was dependent on the product of the uvrA gene, and the presence of cross-links in DNA from MEV-treated bacteria.
Subject(s)
Anti-Bacterial Agents/pharmacology , DNA Replication/drug effects , Nitroimidazoles/pharmacology , DNA Repair , DNA, Bacterial/biosynthesis , Drug Resistance, Microbial , Escherichia coli/drug effects , Mutation/drug effects , Nucleic Acid DenaturationABSTRACT
Although the target of the antimicrobial drug 1-methyl-2-nitro-5-vinylimidazole (MEV) has been shown to be DNA (Goldstein et al., 1977) the drug was ineffective in cell-free systems because it was not activated. Both the rate of metabolic activation of MEV and its antibacterial activity were increased when bacteria were grown in limiting oxygen. Mutants of Escherichia coli which were conditionally resistant to nitroimidazoles and nitrofurans were defective in drug activation. The activities of these drugs against E. coli correlated with their rates of metabolism. The antimicrobial spectrum of the drugs appeared to be related to their reducibility by different species.
Subject(s)
Anti-Bacterial Agents/metabolism , Escherichia coli/metabolism , Aerobiosis , Culture Media , DNA, Bacterial/biosynthesis , Drug Resistance, Microbial , Escherichia coli/drug effects , Nitrofurans/pharmacology , Nitroimidazoles/pharmacologyABSTRACT
The presence of antibodies to rifampin was determined in sera of patients with tuberculosis on daily treatment one day after discontinuation of treatment, one week later, 3 weeks later, and 8 weeks later. The highest number of positive patients was found in the third week.
Subject(s)
Antibody Formation , Rifampin/immunology , Tuberculosis/drug therapy , Animals , Antigen-Antibody Reactions , Humans , Rats , Rifampin/administration & dosage , Rifampin/therapeutic use , Time Factors , Tuberculosis/bloodABSTRACT
The new antibiotic gardimycin has an interesting in vitro antibacterial activity against Gram-positive bacteria and Neisseria gonorrhoeae. Parenteral administration gives a high degree of protection against experimental infections in mice. It also shows some chemotherapeutic activity when given rectally.
Subject(s)
Anti-Bacterial Agents/pharmacology , Actinomycetales/metabolism , Animals , Anti-Bacterial Agents/analysis , Anti-Bacterial Agents/therapeutic use , Bacteria/drug effects , Bacterial Infections/drug therapy , Culture Media , Drug Resistance, Microbial , Drug Stability , Female , Hydrogen-Ion Concentration , Male , Mice , Microbial Sensitivity Tests , Peptides/analysis , Peptides/pharmacology , Peptides/therapeutic use , Time Factors , Yeasts/drug effectsABSTRACT
In sera of patients treated with rifampicin, who had episodes ascribable to rifampicin sensitization, IgE antibodies were found. These antibodies cross-react with rifamycin SV and with the chromophoric moiety of rifamycins, but not with the side chain of rifampicin.
Subject(s)
Antibodies/analysis , Drug Hypersensitivity/immunology , Immunoglobulin E/analysis , Rifampin/adverse effects , Histamine Release , Humans , Rifampin/immunology , Rifamycins/pharmacologyABSTRACT
Twenty-three volunteer patients with pulmonary tuberculosis were studied for the effect of rifampin on humoral immunity. The patients were matched for age and body weight with other patients not on rifampin treatment. Antibodies to influenza virus were measured 18 days after immunization. At the clinical doses used and for the periods of treatment between 3 and 8 months no difference in humoral immune response was observed between patients treated with rifampin and the control subjects. All but one treated patient showed an increase of antibody titers after vaccination.
Subject(s)
Antibody Formation/drug effects , Orthomyxoviridae/immunology , Rifampin/therapeutic use , Tuberculosis, Pulmonary/drug therapy , Adult , Aged , Antigens, Viral , Humans , Male , Middle Aged , Rifampin/pharmacology , Tuberculosis, Pulmonary/immunologyABSTRACT
1. Rifamazine (AF/RP) a dimeric rifamycin, is active against bacterial DNA-dependent RNA polymerase and against viral RNA-dependent DNA polymerase. 2. Rifamazine is active also against DNA-dependent RNA polymerase extracted from rifampicin-resistant mutants of Escherichia coli. It does not interfere with enzyme-template interaction or with RNA elongation. It blocks initiation. 3. A comparison is made between the mechanism of action of rifamazine and that of rifampicin, and of AF/013 (octyloxime of 3-formylrifamycin SV), a C-class rifamycin. Our results show that the mechanism of action of rifamazine is more similar to that of rifampicin than to that of the octyloxime derivative. 4. Activity of rifamazine against RNA polymerase from rifampicin-resistant mutants is thought to be due to binding of the dimer to both the rifamycin-specific binding site and to a second weak site.
Subject(s)
DNA-Directed RNA Polymerases/metabolism , RNA-Directed DNA Polymerase/metabolism , Rifamycins/pharmacology , Coliphages , DNA Viruses , DNA, Viral , Drug Resistance, Microbial , Escherichia coli/drug effects , Escherichia coli/metabolism , Kinetics , Mutation , Rifampin/pharmacology , Structure-Activity Relationship , Transcription, Genetic/drug effectsABSTRACT
Among 117 gram-negative bacteria isolated from pathological materials, 46 were found to carry antibiotic resistances transferable to Escherichia coli K-12; we therefore concluded that they carry infectious R factors. When transferred to a type of rifampin-resistant mutant of E. coli, all these R factors decreased the resistance to rifampin, but only 10% of them lowered the resistance to one-tenth or less that of the isogenic R(-) strain. The relevance of these facts for the epidemiology of R factors in gram-negative bacteria is discussed.
Subject(s)
Escherichia coli/genetics , R Factors , Rifampin/pharmacology , Escherichia coli/drug effects , MutationSubject(s)
Antibody Formation/drug effects , Escherichia coli/immunology , Immunosuppression Therapy , Rifampin/pharmacology , Animals , Antibody-Producing Cells , Cholera Vaccines , Erythrocytes/immunology , Hemolytic Plaque Technique , Humans , Lipopolysaccharides , Macrophages/drug effects , Mice , Phagocytosis/drug effects , Polysaccharides, Bacterial , Rifampin/toxicity , Sheep/immunologySubject(s)
DNA-Directed RNA Polymerases/biosynthesis , Escherichia coli/enzymology , Genes , Mutation , Rifampin , Chromosome Mapping , Coliphages/growth & development , Crosses, Genetic , Drug Resistance, Microbial , Escherichia coli/drug effects , Lysogeny , Phenotype , Transcription, Genetic , Transduction, GeneticABSTRACT
Subinhibitory doses of rifampin cured F(+)Escherichia coli cells from the episome. The target of the drug was transcription because E. coli mutants with a ribonucleic acid polymerase resistant to rifampin were not cured. The experimental conditions required for optimal curing with rifampin very closely resembled those required for curing with acridine orange. Mutants were found which are more resistant to curing by both acridine orange and rifampin. Probably the two drugs affect a common metabolic step, or alternatively they may inhibit the synthesis of a factor which is necessary for the replication of the episome.
Subject(s)
Acridines/pharmacology , Escherichia coli/drug effects , Extrachromosomal Inheritance/drug effects , Rifampin/pharmacology , Drug Resistance, Microbial , Mutation , RNA, Bacterial , Sex , Time FactorsSubject(s)
Antibody Formation/drug effects , Antibody-Producing Cells/drug effects , Erythrocytes/immunology , Rifampin/pharmacology , Animals , Antigens, Bacterial , Depression, Chemical , Hemagglutination Tests , Hemolytic Plaque Technique , Immunoglobulin G/biosynthesis , Injections, Intraperitoneal , Mice/immunology , Phagocytosis/drug effects , Rabbits/immunology , Rifampin/administration & dosage , Sheep , Time FactorsSubject(s)
DNA-Directed RNA Polymerases/antagonists & inhibitors , Rifamycins/pharmacology , Alanine Transaminase/antagonists & inhibitors , Alkaline Phosphatase/antagonists & inhibitors , Animals , Aspartate Aminotransferases/antagonists & inhibitors , Cattle , Centrifugation, Density Gradient , Chemical Phenomena , Chemistry , DNA Nucleotidyltransferases/analysis , DNA-Directed RNA Polymerases/analysis , Drug Resistance, Microbial , Escherichia coli/enzymology , Microbial Sensitivity Tests , Mutation , Rifampin/pharmacology , Serum Albumin, Bovine/pharmacology , Sucrose , Templates, GeneticSubject(s)
Rifamycins , Bacteria/drug effects , Bacteria/enzymology , Bacteriophages/drug effects , Chemical Phenomena , Chemistry , DNA Viruses/drug effects , DNA, Bacterial , DNA-Directed RNA Polymerases/metabolism , Drug Resistance, Microbial , Eukaryota/drug effects , Extrachromosomal Inheritance/drug effects , Fungi/drug effects , Genetics, Microbial , Mutation , Oncogenic Viruses/drug effects , RNA Viruses/drug effects , Rifamycins/pharmacology , Transcription, GeneticSubject(s)
Drug Resistance, Microbial , Escherichia coli , Genetics, Microbial , Rifampin/pharmacology , Carbon Isotopes , Crosses, Genetic , Dactinomycin/pharmacology , Erythromycin/pharmacology , Escherichia coli/drug effects , Escherichia coli/enzymology , Escherichia coli/metabolism , Microbial Sensitivity Tests , Mutation , RNA Nucleotidyltransferases/antagonists & inhibitors , RNA, Bacterial/biosynthesis , Recombination, Genetic , Uracil/metabolismABSTRACT
Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) isolated from a rifampin-sensitive strain of Mycobacterium smegmatis was 90% inhibited by 1 mug of rifampin per ml; enzyme from a rifampin-resistant mutant was not affected by this concentration of antibiotic. Inhibition of phenylalanine-1-(14)C incorporation by rifampin in growing cultures was complete about 6 min after addition of antibiotic. Under the same conditions, uracil-2-(14)c incorporated was blocked after 1.5 to 2 min. Rifampin kills M. smegmatis very slowly. When rifampin-inhibited cultures were transferred to a rifampin-free medium, there was a partial resumption of uracil-2-(14)C incorporation, even in the presence of chloramphenicol. We conclude that a primary event in the inhibition of M. smegmatis by rifampin is the block of DNA-dependent RNA polymerase.