Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction.

OBJECTIVE: To investigate how and to what extent platycodin saponin (PS) from Platycodi Radix exerts a favorable influence on obesity and hyperlipidemia. DESIGN: Sprague-Dawley rats were fed with a high fat (HF) diet for 4 weeks and then the animals were treated with 35 or 70 mg / kg of PS for another 4 weeks. Changes in body weight and daily calorie intake were measured regularly during the experimental period and the degree of linear correlation for the above two variables was further analyzed. The in vitro lipase inhibition of each PS compound and the in vivo fecal lipid excretion were examined in hope of revealing their relationship. The concentrations of hepatic triglyceride and cholesterol in serum. RESULTS: The body weight reduction (13+/-4% vs HF control, P<0.05) by PS administration was highly correlated to the food intake restriction (Pearson's linear coefficient r=0.752, P<0.005). The in vitro inhibition of lipase by each isolated compound and mixture of PS were virtually identical. Consequently, the fecal TG excretion was increased by 2.1-3.2 folds depending on the dose of PS. The serum TG and LDL-cholesterol concentrations were decreased without noticeable changes in HDL-cholesterol levels. Concomitantly, the contents of the hepatic TG, cholesterol, and the liver surface fat pads were decreased in ubiquity, but no noticeable biochemical abnormalities or histological tissue damages were observed. CONCLUSIONS: The administration of PS produced profound effects on the control of obesity and lipid metabolism, which resulted in LDL-cholesterol reduction. PS also caused a remarkable reduction in calorie intake, which was highly correlated to the body weight loss. These results suggest that PS has a greater role in anti-obesity, hypolipidemia, and liver protection than previously thought. Hence, PS could be a potential therapeutic alternative in the treatment of obesity and hyperlipidemia.

Maternal dietary ratio of linoleic acid to alpha-linolenic acid affects the passive immunity of hatching chicks.

The objective of the current study was to examine the effect of dietary ratio of linoleic acid (LA) to alpha-linolenic acid (LNA) on the humoral immune response in laying hens and further on the passive immunity of their progeny. Thirty-two Single Comb White Leghorn laying hens, 24 wk of age, were randomly assigned to 1 of 4 diets that had LA-to-LNA ratios of 0.8, 5.4, 12.5, and 27.7, respectively, by changing the proportions of sunflower and linseed oils. After 5 wk on the experimental diets, hens were immunized intramuscularly with 1 mg of BSA, followed by 2 boosters 2 and 6 wk later. Serum and egg yolk were obtained weekly from 0 to 6 wk following the first injection of BSA. One week after the second booster, fertile eggs were collected and incubated. The sera of 11-d-old embryos and hatchlings were collected. All serum samples were stored at -20 degrees C before analysis. The results showed that dietary LA-to-LNA ratio had no effect on the total IgG and BSA-specific antibody IgG concentrations in the serum or egg yolk of laying hens. Hatchlings from hens fed the diet containing the LA-to-LNA ratio of 12.4 showed lower (P < 0.05) BSA-specific IgG titer in the serum than those from hens given the diet containing LA-to-LNA ratio of 0.8. A lower (P < 0.05) total IgG concentration was observed in hatchlings from hens fed the diet containing LA-to-LNA ratio of 12.4 compared with those from hens fed diets containing 0.8 and 5.4 of LA-to-LNA ratios. It is suggested that the dietary ratio of LA to LNA has no effect on laying hen humoral response but affects the passive immunity of hatching chicks.

Maternal diet with diverse omega-6/omega-3 ratio affects the brain docosahexaenoic acid content of growing chickens.

Eggs with diverse omega-6/omega-3 ratio produced by feeding breeder hens a wheat-soybean meal-basal diet containing 5% (wt/wt) sunflower oil (H(omega)6), 5% fish oil (H(omega)3) or 2.5% sunflower oil plus 2.5% fish oil (M(omega)3omega6) were incubated. The hatched chicks were fed a docosahexaenoic acid (DHA)-deficient diet up to 6 weeks of age. The fatty acid composition of chick brain was determined on 0, 2, 4 and 6 weeks and brain weight was taken on day 0 and day 42. The omega-6/omega-3 ratios were 37.12, 4.21 and 0.98 for the maternal diet; 28.36, 2.83 and 0.89 for the egg yolk; 1.94, 0.48 and 0.18 for hatched chick brain (p < 0.05). At 2 weeks of age, the omega-6/omega-3 ratios were 1.88, 0.81 and 0.60 for chicks hatched from hens fed H(omega)6, M(omega)3omega6 and H(omega)3 diets, respectively. The brain DHA contents at 0 and 2 weeks of age were Homega3 > M(omega)3omega6 < H(omega)6 (p < 0.05) and at 4 and 6 weeks of age H(omega)3 = M(omega)3omega6 > H(omega)6. Dietary C18:3omega3 in the starter and finisher diet did not increase brain DHA (p > 0.05). The significant increase in the content of C22:5omega3 at 6 weeks of age in group 1 birds with a concomitant reduction in DHA suggests a weak delta-4 desaturation but an effective delta-6 and delta-5 desaturation similar to human infants. Considering the role of DHA in early brain development and growth, the maternal supply of DHA during growth might be of importance when fed a DHA-deficient neonatal diet.

Maternal and posthatch dietary polyunsaturated fatty acids alter tissue tocopherol status of chicks.

The effects of maternal and starter diet polyunsaturated fatty acid (PUFA) composition on the tocopherol (TOC) status of posthatch chicks were investigated. Fertile eggs enriched with long chain n-3, 18:3 n-3 or 18:2 n-6 PUFA were incubated. The eggs were collected from hens fed diets containing 3.5% menhaden oil (MO), linseed oil (LO), or sunflower oil (SO) and a vitamin E mix containing 400 microg/g total TOC. Posthatch chicks from MO, LO, or SO were fed starter diets containing 3.5% MO, LO, or SO along with vitamin E mix containing 48 microg/g total TOC. Tissues (liver, blood, brain) were collected on d 0 (day of hatch), 7, 14, and 21 posthatch. On d 0, MO chicks had the lowest liver and plasma TOC (P < 0.05). A rapid depletion of liver and plasma TOC was observed on d 7 and 14 posthatch (P < 0.001) and was lower in MO chicks (P < 0.05) than LO. When compared with d 0, a 98% decrease of tocopherol on d 7 was observed for chicks from all treatments. No changes due to age or diet PUFA was observed in the brain TOC status. Data showed that maternal and starter diet PUFA could alter the TOC status of chicks in early life.

Maternal dietary FA modulate the long-chain n-3 PUFA status of chick cardiac tissue.

The effect that egg yolk or maternal n-3 FA have on the cardiac tissue long-chain n-3 FA status of chicks during growth was investigated. Eggs with low, medium, and high levels of n-3 PUFA were obtained by feeding breeder hens a wheat/soybean meal-based diet containing 5% sunflower oil (Low n-3), 2.5% sunflower oil plus 2.5% fish oil (Medium n-3), or 5% fish oil (High n-3). The chicks hatched from Low, Medium, and High n-3 eggs were fed a diet containing 18:3n-3, but devoid of long-chain n-3 FA. The FA composition of cardiac tissue was determined on days 0, 14, 28, and 42. At day 0, the cardiac FA reflected maternal diet. With time, the level of all the long-chain n-3 FA decreased compared with day 0, and this was true especially by day 14. These data show that dietary 18:3n-3 fed to the chicks did not sustain high levels of EPA and DHA in cardiac tissue, despite the high content of long-chain n-3 FA in the maternal diet. At days 0 and 14, the chicks hatched from High and Medium n-3 eggs had higher 20:5n-3, 22:5n-3, and 22:6n-3 contents with a concomitant reduction in 20:4n-6 in the cardiac tissue compared with the Low n-3 egg group. Cardiac tissue of birds hatched from Medium n-3 eggs retained higher levels of 20:5n-3 up to day 42 of growth when compared with other treatments (P < 0.05). None of the treatments was effective in maintaining DHA levels after day 14 of growth.

Maternal dietary N-3 fatty acids alter the spleen fatty acid composition and bovine serum albumin-induced wing web swelling in broilers.

Effects of maternal dietary polyunsaturated fatty acids (PUFA) on the spleen fatty acid composition and BSA-induced wing web swellings were investigated in broilers. One hundred twenty broiler breeder hens 26 wk of age were randomly assigned to diets containing mainly wheat, corn, soy meal, barley, oat and 5% (wt/wt) added sunflower oil, fish oil, or a mix of sunflower and fish oils (1:1). After 2 wk on the experimental diets, birds were inseminated, eggs were collected and incubated. Progeny chicks were then fed identical diets for 6 wk. The maternal dietary oils affected (P < 0.05) n-6 and n-3 PUFA in the spleens of hatching chicks. After 2 wk, n-6 PUFA did not differ among the groups; n-3 PUFA, docosapentaenoic, and docosahexaenoic (DHA) acids were higher (P < 0.05) in the spleens of broilers from hens fed 2.5 or 5% fish oil. After 4 wk, broilers from hens fed 5% fish oil still had higher levels of DHA (P < 0.05) in their spleens than those from hens fed 5% sunflower oil. The BSA-induced wing web swelling response was suppressed (P < 0.05) by n-3 PUFA in breeder hens. Broilers from hens fed high levels of n-3 PUFA had lower (P < 0.05) wing web swelling reactions to BSA at 2 wk (2.5% fish oil) and 4 wk (2.5 and 5% fish oil). In conclusion, n-3 PUFA in breeder hen diets suppressed the BSA-induced wing web swellings of the hens, increased the spleen n-3 fatty acids (especially DHA), and decreased BSA-induced wing web swellings of progeny up to 4 wk of age.

In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium.

Chicken egg yolk antibody (IgY) raised against Salmonella enteritidis or Salmonella typhimurium was found in highly specific activity levels by ELISA. S. enteritidis- and S. typhimurium-specific IgY powder, prepared by freeze-drying the egg yolk water-soluble fraction, contained 15.5 and 10.0% of specific IgY, respectively. Anti-S. enteritidis IgY cross-reacted 55.3% with S. typhimurium. The cross-reactivity of anti-S. typhimurium IgY with S. enteritidis was 42.4%. Salmonella-specific IgY was demonstrated to inhibit Salmonella growth in liquid medium. The growth rate of S. enteritidis incubated with S. enteritidis-specific IgY was fourfold less than that of the control group during a 4-to-6-h incubation. Cell counts of S. typhimurium incubated with S. typhimurium-specific IgY were reduced by 1.6 log cfu/mL in comparison to that of the control group after 6 h of incubation. The specific binding activity of IgY was further evaluated by using immunofluorescence and immunoelectron microscopy. It was found that Salmonella-specific IgY could bind to the antigens expressed on the Salmonella surface, resulting in structural alterations of the bacterial surface.

Detection of segmental branch renal artery stenosis by Doppler US: a case report.

In stenosis of a segmental branch or among multiple renal arteries, Doppler sampling of intrarenal arteries in the upper, mid and lower poles demonstrates strikingly different waveform patterns that might otherwise be overlooked. We report a case of segmental branch renal artery stenosis in which a pulsus parvus et tardus waveform was observed in a segmental branch of a renal artery. In this case, systematic analysis of Doppler waveforms of intrarenal arteries at more than three different locations facilitated a rapid and confident diagnosis of segmental branch renal artery stenosis.

Maternal dietary alpha-linolenic acid (18:3n-3) alters n-3 polyunsaturated fatty acid metabolism and liver enzyme activity in hatched chicks.

We investigated the effects of dietary alpha-linolenic acid (LNA; 18:3n-3) of laying hens on the fatty acid composition of liver microsomes and activity of delta-6 desaturase in hatched chicks. Laying hens were fed wheat-soybean meal-based diets with (Flax) or without ground flax (control). At Day 21 of feeding, fertile eggs were collected and incubated. On the day of hatching, chicks (n = 6) were killed, and liver microsomes were isolated and assayed for delta-6 desaturase enzyme activity. The total n-3 fatty acids in the Flax eggs was 13.0% compared with 2.3% (P < 0.001) in the control group. LNA was the major n-3 fatty acid in the Flax eggs and constituted 10%. The long-chain n-3 polyunsaturated fatty acids (PUFA) (20:5n-3, 22:5n-3, and 22:6n-3) constituted 3.1% in Flax eggs compared with 1.5% in control eggs. Docosahexaenoic acid (22:6n-3) composed 2.1 and 1.2% in Flax and control eggs (P < 0.05), respectively. The liver microsomes of Flax chicks incorporated higher 18:3n-3, 20:5n-3, and 22:5n-3 (P < 0.05) with a concomitant reduction in 20:4n-6, 22:4n-6, and 22:5n-6 compared with control chicks (P < 0.05). The delta-6 desaturase activities in Flax and control groups were 49.4 and 82.8 pmol/min per mg of protein, respectively (P < 0.05). These results demonstrate the role of maternal and yolk n-3 fatty acids in modulating the activity of rate-limiting enzymes for PUFA synthesis in hatched chicks.

Dietary palm oil alters the lipid stability of polyunsaturated fatty acid-modified poultry products.

Laying hens were fed a high polyunsaturated fatty acid (PUFA) diet with three levels [low (LPO, 0%), medium (MPO, 1.5%), and high (HPO, 3.5%)] of palm oil (PO) or a PUFA diet with tocopherol mix (control). Flax and fish oils were used as PUFA sources in all of the diets. Inclusion of tocopherols resulted in a lower (P < 0.05) thiobarbituric acid reactive substances (TBARS) in the control eggs in white and dark meats. Among the PO treatments, eggs from HPO diet had the lowest TBARS (P < 0.05). A higher (P < 0.05) TBARS was observed for eggs, meat, and liver from hens on the LPO diet. No difference was observed between the TBARS of LPO and MPO dark meat. The C22:6 n-3 fatty acid was lower (P < 0.05) in MPO and HPO eggs and the HPO white meat.

Interface vessels on color/power Doppler US and MRI: a clue to differentiate subserosal uterine myomas from extrauterine tumors.

PURPOSE: In subserosal myomas, vessels are often demonstrated between the masses and the uterus. This study was performed to assess the usefulness of demonstrating these vessels in differentiating subserosal myomas from extrauterine tumors on color or power Doppler US (CDUS/PDUS) and MRI. METHOD: This retrospective study included 41 patients with subserosal myomas and 27 patients with solid extrauterine tumors. The incidence and shape of these vessels seen on CDUS/PDUS and MRI were compared in the myoma and extrauterine tumor groups. RESULTS: The interface vessels were demonstrated in 39 of 41 subserosal myomas (18 on CDUS/PDUS, 14 on MRI, 7 on both), whereas they were seen in only 3 of 27 extrauterine tumors (1 on CDUS/PDUS, 2 on MRI). These three extrauterine tumors were ovarian malignancies that directly invaded the uterus. The shapes of these interface vessels were 7 intervening, 12 crossing, and 20 mixed in the myoma group, whereas they were mixed in all three extrauterine tumor groups. The sensitivity/specificity of this finding in differentiating subserosal myomas and extrauterine tumors was 100/92%, 91/91%, and 95/89%, respectively, with CDUS/PDUS, MRI, and either CDUS/PDUS or MRI. CONCLUSION: Observation of the interface vessels between the uterus and juxtauterine masses seems to be a useful clue in differentiating subserosal myomas from extrauterine tumors.

A Case of Well-differentiated Papillary Mesothelioma Developing Malignant Mesothelioma with Seeding Mass on the Trocar Insertion Site of Diagnostic Laparoscopy and Malignant Change.

Although well-differentiated papillary mesothelioma (WDPM) is usually classified as benign, the natural history of this lesion has not been clearly established. We present a case of WDPM in 60-year old woman developing malignant mesothelioma with seeding mass on the trocar insertion site over a period of 2 years. The initial symptom exhibited by the patient was abdominal distension from massive ascitic fluid. With an impression of peritoneal carcinomatosis, we performed a diagnostic laparoscopy. On the laparoscopic finding, a small whitish nodule was found on the liver surface and the pathologic result revealed reactive mesothelial hyperplasia. At exploro-laparotomy, multiple small nodules were found on the omentum and a biopsy revealed well-differentiated papillary mesothelioma of the peritoneum. The patient underwent pelvic lymphadenectomy and omentectomy of the colon and was followed for 2 years without any further treatment. Subsequently, she presented with abdominal distention with massive ascites and palpable abdominal wall mass at the previous trocar insertion site. Malignant mesothelioma was confirmed histologically via re- exploration. The rare transformation of well-differentiated papillary mesothelioma into a typically malignant diffuse mesothelioma and the unusual seeding on trocar insertion site prompted us to report this case.

Fatty acid determination in chicken egg yolk: a comparison of different methods.

Four different methods (direct-methylation, saponification, chloroform-methanol extraction, and postextraction saponification) were compared to determine the fatty acids in egg yolk. About 50 mg of pooled egg yolk samples, with C23:0 as an internal standard, was used for all assays. No difference (P > 0.05) was observed among the four methods for C17:0, C18:0, C18:1, C20:1, C18:2n-6, and C22:6n-3 content of egg yolk. Direct saponification resulted in a lower (P < 0.05) content of C14:0, C16:1, C18:3n-3, and C20:4n-6. Fatty acids at less than 0.5%, such as C15:0 and C14:1, were not detectable in the direct saponification method. The total saturated, monounsaturated, or polyunsaturated fatty acids did not differ (P > 0.05) among the four methods. Direct methylation of egg yolk resulted in lower variability than other methods and is fast and economic for determining egg fatty acid composition.

Dietary polyunsaturated fatty acids alter lymphocyte subset proportion and proliferation, serum immunoglobulin G concentration, and immune tissue development in chicks.

The effects of fat source on immune response of the offspring of the Single Comb White Leghorn laying hens were investigated. The laying hens were fed for 6 wk with a wheat-soybean meal basal diet with added sunflower oil (SO), animal oil (AO), linseed oil (LO), or menhaden fish oil (FO) at 5% (wt/wt). Upon hatching, the chicks (30/group) were given the same types of diets for 8 wk. The dietary SO, AO, and LO provided different n-6 to n-3 polyunsaturated fatty acids (PUFA) ratios. The FO and LO had ratios of n-6 to n-3 PUFA that were close but had different components of n-3 PUFA. The results demonstrated that the chicks fed LO or FO had significantly lower (P < 0.05) splenocyte proliferative response to ConA than the chicks fed SO or AO at either 4 wk or 8 wk of age, with a stronger (P < 0.05) suppressive effect produced by LO at 4 wk. A significantly lower (P < 0.05) splenocyte response to PWM was produced by the chicks fed AO, LO, and FO compared with the chicks fed SO at 8 wk. The thymus lymphocyte proliferation in response to ConA at 4 wk was lower (P < 0.05) in the chicks fed AO, LO, and FO than in the chicks fed SO. Both LO and FO elevated (P < 0.05) the proportion of IgM+ lymphocytes in spleen, but only FO increased (P < 0.05) the serum IgG concentration. The LO elevated (P < 0.05) the percentage of CD8+ T-lymphocytes but not the ratio of CD4+ to CD8+ cells (P > 0.05) in spleen. Growths of thymus, spleen, and bursa were impacted significantly (P < 0.05) by the amount of dietary PUFA, the ratio of n-6 to n-3 fatty acids, and n-3 PUFA components.

Recurrent uterine cervical carcinoma: spectrum of imaging findings.

Uterine cervical carcinoma is one of the most common malignant tumors occurring in females. After primary treatment, patients are usually followed up with CT or MRI and the findings of these modalities may be the first sign of recurrent disease. Because earlier additional treatment by chemotherapy or radiation therapy may improve the prognosis, the early detection of recurrent cervical carcinoma is clinically important. In this article, we review the CT and MR imaging findings of recurrent uterine cervical carcinoma, and assign them to one of four groups: a) recurrence at the primary site, involving the intrapelvic organs, b) extension to the pelvic side-wall, c) metastases to pelvic and extrapelvic lymph nodes, or d) metastases to distant organs. A further contribution of CT and MR imaging is the detection of hydronephrosis due to ureteral obstruction. The cases in each group are illustrated and discussed, and since an awareness of the spectrum of imaging findings of recurrent cervical carcinoma is likely to lead to its early detection, radiologists should be familiar with the information presented.

Differentiation of small hyperechoic renal cell carcinoma from angiomyolipoma: computer-aided tissue echo quantification.

To assess the value of computer-aided tissue echo quantification in differentiating small hyperechoic renal cell carcinomas from angiomyolipomas, we studied ultrasonographic images of 15 renal cell carcinomas and 20 angiomyolipomas. After digitizing the images, we measured the absolute gray scale values of the renal cortex, central echo complex, and mass. The relative gray scale value (%) of the mass was calculated by setting the gray scale value of the cortex as 0% and the central echo complex as 100%. The relative gray scale value of renal cell carcinomas was in the range of 12 to 73% (mean, 28%), whereas that of angiomyolipomas was 30 to 204% (mean, 130%). The differentiation between small hyperechoic renal cell carcinomas and angiomyolipomas can be facilitated by computer-aided tissue echo quantification.

Definitive diagnosis of intestinal volvulus in utero.

Midgut volvulus with or without intestinal malrotation can occur in fetal life. Several reports have described congenital midgut volvulus showing non-specific sonographic findings of intestinal obstruction and perforation in utero. None of the previously reported cases, however, were definitively diagnosed as midgut volvulus by fetal sonography. We report two cases both exhibiting the sonographic 'whirlpool' sign, in utero. Color Doppler interrogation provided a clue to the viability of the involved intestinal segment.

Control of clonorchiasis by repeated praziquantel treatment and low diagnostic efficacy of sonography.

In Korea, Clonorchis sinensis infection is still highly prevalent because case detection in the field is difficult and the detected cases used to be incompletely cured due to treatment failure. The present study tried to control clonorchiasis in an endemic village by repeated treatments with praziquantel every 6 months and to evaluate sonography as a diagnostic measure. By stool examinations, the egg positive rate in the endemic village was 22.7%, but it decreased to 19.6% at 6 months, 15.1% at 12 months. 12.2% at 18 months, 6.3% at 24 months, 11.4% at 30 months, and 6.3% at 42 months after the beginning of repeated praziquantel administration. The sonography showed 61 (49.6%) positive cases of 123 screened residents: among egg-positives the sonography positive rate was 52.2% and among egg-negatives it was still 49%. The rate among cured cases was 64.3% after 6 months, 50.0% after 12 months, 50.0% after 18 months, and 66.7% after 24 months. In a non-endemic village, 64 residents were found egg-negative by fecal examination, but 20 (31.3%) of them were positive by sonography. The present findings indicate that control of clonorchiasis in an endemic village by repeated praziquantel treatment for 42 months is still insufficient and sonography is of little value for diagnosis of clonorchiasis.