Scientific contributions of citizen science applied to rare or threatened animals.

Citizen science is filling important monitoring gaps and thus contributing to the conservation of rare or threatened animals. However, most researchers have used peer-reviewed publications to evaluate citizen science contributions. We quantified a larger spectrum of citizen science's contributions to the monitoring of rare or threatened animals, including contributions to the peer-reviewed publications, gray literature and to conservation measures (i.e., actions taken as a direct result of citizen science monitoring). We sought to provide broad information on how results of studies of citizen science monitoring is used. We also evaluated factors associated with success of citizen science projects. We conducted a web search to find citizen science projects focusing on rare and threatened species and surveyed citizen science project managers about their contributions and factors influencing their success. The number of projects increased rapidly after 2010. Almost one-half of the citizen science projects produced at least 1 peer-reviewed publication, 64% produced at least 1 gray literature publication, and 64% resulted in at least 1 conservation measure. Conservation measures covered a wide range of actions, including management and mitigation plans, modification of threat status, identification and establishment of protected areas, habitat restoration, control of invasive species, captive breeding programs, and awareness campaigns. Longevity, data quality, and collaboration type best explained quantities of all types of scientific contributions of citizen science. We found that citizen science contributed substantially to knowledge advancement and conservation, especially when programs were long term and had rigorous data collection and management standards, and multidisciplinary or transdisciplinary collaborations.

La ciencia ciudadana contribuye a llenar vacíos en el monitoreo, lo que ayuda a la conservación de animales raros o amenazados. Sin embargo, la mayoría de los investigadores han usado publicaciones revisadas por pares para evaluar las contribuciones de la ciencia ciudadana. Cuantificamos un mayor espectro de las contribuciones de la ciencia ciudadana al monitoreo de animales raros y amenazados, incluyendo las contribuciones a la literatura gris, a las publicaciones revisada por pares y a las medidas de conservación (es decir, las acciones tomadas como resultado directo del monitoreo ciudadano). Buscamos proporcionar información generalizada sobre cómo los resultados de los estudios de monitoreo ciudadano es usado. También evaluamos los factores asociados con el éxito de los proyectos de ciencia ciudadana. Realizamos una búsqueda en línea para encontrar proyectos de ciencia ciudadana enfocados en especies raras o amenazadas y encuestamos a los gestores de estos proyectos sobre sus contribuciones y los factores que influyen sobre su éxito. El número de proyectos incrementó rápidamente a partir de 2010. De los proyectos de ciencia ciudadana, casi la mitad produjo al menos 1 publicación revisada por pares, el 64% produjo al menos una publicación en la literatura gris y el 64% derivó en al menos 1 medida de conservación. Las medidas de conservación abarcaron una gama extensa de acciones que incluyeron planes de gestión y mitigación, modificación del estado de amenaza, identificación y establecimiento de áreas protegidas, restauración del hábitat, control de especies invasoras, programas de reproducción en cautiverio y campañas de concientización. La longevidad, calidad de los datos y el tipo de colaboración explicaron de mejor manera las cantidades de todos los tipos de contribuciones científicas hechas por la ciencia ciudadana. Descubrimos que la ciencia ciudadana contribuyó sustancialmente al avance del conocimiento y la conservación, especialmente cuando los programas eran a largo plazo y contaban con estándares rigurosos de recolección y gestión de datos, y con colaboraciones multi o transdisciplinarias.

Using mounting, orientation, and design to improve bat box thermodynamics in a northern temperate environment.

Wildlife managers design artificial structures, such as bird houses and bat boxes, to provide alternative nesting and roosting sites that aid wildlife conservation. However, artificial structures for wildlife may not be equally efficient at all sites due to varying climate or habitat characteristics influencing thermal properties. For example, bat boxes are a popular measure employed to provide compensatory or supplementary roost sites for bats and educate the public. Yet, bat boxes are often thermally unstable or too cold to fulfill reproductive females needs in northern temperate environments. To help improve the thermodynamics of bat boxes, we tested the effect of (1) three mountings, (2) four orientations, and (3) twelve bat box designs on the internal temperature of bat boxes. We recorded temperatures in bat boxes across a climate gradient at seven sites in Quebec, Canada. Bat boxes mounted on buildings had warmer microclimates at night than those on poles and those facing east warmed sooner in the morning than those facing west or south. Our best new model based on passive solar architecture (Ncube PH1) increased the time in the optimal temperature range (22-40 °C) of targeted species by up to 13% compared to the most commonly used model (Classic 4-chamber) when mounted on a building with an east orientation (other designs presented in the Supplementary Information). Based on bioenergetic models, we estimated that bats saved up to 8% of their daily energy using the Ncube PH1 compared to the Classic 4-chamber when mounted on a building with an east orientation. We demonstrate that the use of energy-saving concepts from architecture can improve the thermal performance of bat boxes and potentially other wildlife structures as well.

Long-term changes in bat activity in Quebec suggest climatic responses and summer niche partitioning associated with white-nose syndrome.

In North America, the greatest and most sudden threat to hibernating bats is white-nose syndrome (WNS), which has caused massive declines in populations since 2006. Other determinants of bat dynamics, such as the climate, and the effect of reduction in the number of individuals sharing foraging space and summer roosting habitat may have an effect on population dynamics. We analyzed transect acoustic bat surveys conducted with ultrasonic detectors in 16 regions in Quebec, Canada, between 2000 and 2015. We used piecewise regression to describe changes in activity over time for each species and a meta-analytic approach to measure its association with the North Atlantic Oscillation (NAO). As expected, mouse-eared bat (Myotis spp.) activity sharply declined after the onset of WNS, down by 79% after 3 years. In contrast, big brown/silver-haired bat activity increased over the same period, possibly due to a release of competition. Hoary bats and red bats remained present, although their activity did not increase. Myotis activity was positively correlated with a one-year lag to the NAO index, associated with cold conditions in winter, but warm autumns. Big brown/silver-haired and hoary bats were also more active during NAO-positive years but without a lag. We conclude that combinations of threats may create rapid shifts in community compositions and that a more balanced research agenda that integrates a wider range of threats would help better understand and manage those changes.

Spatial variation of mercury bioaccumulation in bats of Canada linked to atmospheric mercury deposition.

Wildlife are exposed to neurotoxic mercury at locations distant from anthropogenic emission sources because of long-range atmospheric transport of this metal. In this study, mercury bioaccumulation in insectivorous bat species (Mammalia: Chiroptera) was investigated on a broad geographic scale in Canada. Fur was analyzed (n=1178) for total mercury from 43 locations spanning 20° latitude and 77° longitude. Total mercury and methylmercury concentrations in fur were positively correlated with concentrations in internal tissues (brain, liver, kidney) for a small subset (n=21) of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus), validating the use of fur to indicate internal mercury exposure. Brain methylmercury concentrations were approximately 10% of total mercury concentrations in fur. Three bat species were mainly collected (little brown bats, big brown bats, and northern long-eared bats [M. septentrionalis]), with little brown bats having lower total mercury concentrations in their fur than the other two species at sites where both species were sampled. On average, juvenile bats had lower total mercury concentrations than adults but no differences were found between males and females of a species. Combining our dataset with previously published data for eastern Canada, median total mercury concentrations in fur of little brown bats ranged from 0.88-12.78µg/g among 11 provinces and territories. Highest concentrations were found in eastern Canada where bats are most endangered from introduced disease. Model estimates of atmospheric mercury deposition indicated that eastern Canada was exposed to greater mercury deposition than central and western sites. Further, mean total mercury concentrations in fur of adult little brown bats were positively correlated with site-specific estimates of atmospheric mercury deposition. This study provides the largest geographic coverage of mercury measurements in bats to date and indicates that atmospheric mercury deposition is important in determining spatial patterns of mercury accumulation in a mammalian species.

Enrichment of beneficial bacteria in the skin microbiota of bats persisting with white-nose syndrome.

BACKGROUND: Infectious diseases of wildlife are increasing worldwide with implications for conservation and human public health. The microbiota (i.e. microbial community living on or in a host) could influence wildlife disease resistance or tolerance. White-nose syndrome (WNS), caused by the fungus Pseudogymnoascus destructans (Pd), has killed millions of hibernating North American bats since 2007. We characterized the skin microbiota of naïve, pre-WNS little brown bats (Myotis lucifugus) from three WNS-negative hibernation sites and persisting, previously exposed bats from three WNS-positive sites to test the hypothesis that the skin microbiota of bats shifts following WNS invasion. RESULTS: Using high-throughput 16S rRNA gene sequencing on 66 bats and 11 environmental samples, we found that hibernation site strongly influenced the composition and diversity of the skin microbiota. Bats from WNS-positive and WNS-negative sites differed in alpha and beta diversity, as well as in microbiota composition. Alpha diversity was reduced in persisting, WNS-positive bats, and the microbiota profile was enriched with particular taxa such Janthinobacterium, Micrococcaceae, Pseudomonas, Ralstonia, and Rhodococcus. Some of these taxa are recognized for their antifungal activity, and specific strains of Rhodococcus and Pseudomonas are known to inhibit Pd growth. Composition of the microbial community in the hibernaculum environment and the community on bat skin was superficially similar but differed in relative abundance of some bacterial taxa. CONCLUSIONS: Our results are consistent with the hypothesis that Pd invasion leads to a shift in the skin microbiota of surviving bats and suggest the possibility that the microbiota plays a protective role for bats facing WNS. The detection of what appears to be enrichment of beneficial bacteria in the skin microbiota of persisting bats is a promising discovery for species re-establishment. Our findings highlight not only the potential value of management actions that might encourage transmission, growth, and establishment of beneficial bacteria on bats, and within hibernacula, but also the potential risks of such management actions.

Can wide consultation help with setting priorities for large-scale biodiversity monitoring programs?

Climate and other global change phenomena affecting biodiversity require monitoring to track ecosystem changes and guide policy and management actions. Designing a biodiversity monitoring program is a difficult task that requires making decisions that often lack consensus due to budgetary constrains. As monitoring programs require long-term investment, they also require strong and continuing support from all interested parties. As such, stakeholder consultation is key to identify priorities and make sound design decisions that have as much support as possible. Here, we present the results of a consultation conducted to serve as an aid for designing a large-scale biodiversity monitoring program for the province of Québec (Canada). The consultation took the form of a survey with 13 discrete choices involving tradeoffs in respect to design priorities and 10 demographic questions (e.g., age, profession). The survey was sent to thousands of individuals having expected interests and knowledge about biodiversity and was completed by 621 participants. Overall, consensuses were few and it appeared difficult to create a design fulfilling the priorities of the majority. Most participants wanted 1) a monitoring design covering the entire territory and focusing on natural habitats; 2) a focus on species related to ecosystem services, on threatened and on invasive species. The only demographic characteristic that was related to the type of prioritization was the declared level of knowledge in biodiversity (null to high), but even then the influence was quite small.