1.
Gene
; 155(1): 129-30, 1995 Mar 21.
Article
in English
| MEDLINE
| ID: mdl-7698656
ABSTRACT
A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.
Subject(s)
Deoxyribonucleases, Type II Site-Specific/metabolism , Oligodeoxyribonucleotides/metabolism , Base Sequence , Molecular Sequence Data , Streptomyces/enzymology
2.
Gene
; 109(1): 121-3, 1991 Dec 20.
Article
in English
| MEDLINE
| ID: mdl-1756971
ABSTRACT
A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping.
