Study of electrical activity in the area of the hypothalamic ventromedial nucleus and the lateral hypothalamus of rats fed on a high protein and high fat diet.

The authors studied bioelectric potentials in the area of the hypothalamic ventromedial nucleus and the lateral hypothalamus of rats fed on a standard, a high protein and a high fat diet. On the first 3--6 days after changing from the standard to the high fat and high protein diets, a decrease in the amplitude of electrical activity was recorded in both the areas in question. It was also found that the frequency of electrical activity in the hypothalamic ventromedial nucleus or the lateral hypothalamus rose, after 3 days administration of the high fat or the high protein diet, in correlation to the type of diet, and that, in the frequency spectrum, a change occurred in the proportion of basic frequency in relation to superimposed frequencies distorting it. It was further found that there was a permanent difference between the amplitude of electrical activity in the lateral hypothalamus and the hypothalamic ventromedial nucleus.

[Frequency-voltage dependence in the region of the ventromedial hypothalamic nucleus and the lateral hypothalamus of rats fed various diets]. / Frekvencno-napätová závislost oblasti hypothalamického ventromediálneho jadra a laterálneho hypothalamu krýs krmených róznymi diétami.

Adult rats (males) of the Wistar species and of SPF origin were studied for the dependence of frequency on the electric potential on EEG records of the region of the hypothalamic ventromedial nucleus and lateral hypothalamus of animals fed high-protein and high-fat diets. Similar values of control animals were used for comparison. Each group comprised seven animals. In the control animals, constantly fed the basic diet, the values of the frequency-voltage dependence remained unchanged. In the animals fed high-protein diet frequency increased with rising voltage whereas in those fed high-fat diet frequency declined with increasing voltage in both body regions under study.

[Total nitrogen in mice after injections of aurothioglucose]. / Celkový dusík u mysí po injekcii aurothioglukózy

To mature female mice of the "H" strain aurothioglucose was applied, once in the dose of 1 mg g-1 of live weight i. p. In the case of feeding ad libitum we determined, after three months of the experiment, the nitrogen total in the fatfree dry substance. On an average the values of the total N in the animals were, after the injection of aurothioglucose, equal to those of the control animals, and the values of the nitrogen total with regard to the fatfree dry substance were practically the same.

[Gains in body composition and total fat content in mice after administration of aurothioglucose with a limited amount of animal feed]. / Prírastky hmotnosti a celkový obsah tuku u mysí po aplikácii aurothioglukózy pri obmedzenom mnozstve krmiva.

We investigated the effect of a single application of aurothioglucose on the weight and fat content of mice in the case of a limited feed quantity. In the experiment we used 41 mice -- females of the "H" strain, of which 14 served as controls and 27 were experimental mice to which we applied aurothioglucose in the dose of 1 mg g-1 of liveweight, i. p. We found that, under the conditions of feeding a limited quantity of feeds, there occurs in the animals that had been injected with aurothioglucose a statistically significant increase of the weight gains and of the total quantity of fat compared with the control animals.

[Weight gains of rats following injection of aurothioglucose]. / Prírastky hmotnosti krýs po injekcii aurothioglukózy

Aurothioglucose doses of 0.4, 0.5, 0.8, and 1 mg g-1 of live weight were applied to adult female rats of the Wistar strain. No greater weight gain increase was obtained after the injection of ATG in the doses of 0.4, 0.5, and 0.8 mg g-1 l. w. The dose of 1 mg ATG per 1 g l. w. practically represented the LD100 irrespective of application to starving or normally fed rats.

[Development of bioelectric activity in children 7 to 11 years old]. / Die Entwicklung der bioelektrischen Aktivität der Kinder im Alter von 7 bis 11 Jahren.

Three kinds of EEG records may be found in healthy children observed from 7 to 11 years of age. Records with alpha rhythm, where the degree of modulation was differently expressed and asynchroneus theta waves were diffusely localized, are found most frequently. Records with alpha rhythm without addition of slow frequencies and records with synchronized theta waves occur less frequently. Development of records in further years is mostly irregular (in 44% of our series), the individual types given above alternating in the same child without any order. No interrelation was found between the EEG curve and the mental level of children in the framework of healthy population. In children aged 11 years, a greater number of records (in our series 34% as against 10% in children aged 10 years) occurs in which the theta waves in synchronized groups appear again. Relation with pubertal manifestations is not univecal as time synchronization was found in 1 girl only. The case may be that of EEG registered subclinical manifestations of developmental phases of puberty. The incidence of synchronized theta waves which disappears spontaneously in children's later years seems to be connected with the process of maturation. With persistence of this finding in further years, an increased disposition to seizures must be thought of.