Investigation of Protein and Epitope Characteristics of Oats and Its Implications for Celiac Disease.

The use of pure oats (oats cultivated with special care to avoid gluten contamination from wheat, rye, and barley) in the gluten-free diet (GFD) represents important nutritional benefits for the celiac consumer. However, emerging evidence suggests that some oat cultivars may contain wheat gliadin analog polypeptides. Consequently, it is necessary to screen oats in terms of protein and epitope composition to be able to select safe varieties for gluten-free applications. The overall aim of our study is to investigate the variability of oat protein composition directly related to health-related and techno-functional properties. Elements of an oat sample population representing 162 cultivated varieties from 20 countries and the protein composition of resulting samples have been characterized. Size distribution of the total protein extracts has been analyzed by size exclusion-high performance liquid chromatography (SE-HPLC) while the 70% ethanol-extracted proteins were analyzed by RP-HPLC. Protein extracts separated into three main groups of fractions on the SE-HPLC column: polymeric proteins, avenins (both containing three subgroups based on their size), and soluble proteins, representing respectively 68.79-86.60, 8.86-27.72, and 2.89-11.85% of the total protein content. The ratio of polymeric to monomeric proteins varied between 1.37 and 3.73. Seventy-six reversed phase-HPLC-separated peaks have been differentiated from the ethanol extractable proteins of the entire population. Their distribution among the cultivars varied significantly, 6-23 peaks per cultivar. The number of appearances of peaks also showed large variation: one peak has been found in 107 samples, while 15 peaks have been identified, which appeared in less than five cultivars. An estimation method for ranking the avenin-epitope content of the samples has been developed by using MS spectrometric data of collected RP-HPLC peaks and bioinformatics methods. Using ELISA methodology with the R5 antibody, a high number of the investigated samples were found to be contaminated with wheat, barley, or rye.

Drug use among sex workers in Hungary.

Drug use and sex work are both controversial issues with multiple interesting connections. This article presents findings from the first-ever survey on drug use and sex work in Hungary. The study aimed to chart the prevalence, function, and problems of drug use among various groups of sex workers. Survey forms were collected from 510 participants (average age 29.5 years, 91% female) in and near Budapest over a period of six months. The results show that sex workers have manifold higher lifetime prevalence, 84.3%, of illicit drug use compared with the prevalence of the Hungarian general young adult population, 20.9%. In our sample, it was very rare to perform sex work for alcohol or drugs (5%) or for money to purchase alcohol or drugs (20%). Findings also indicate notable relationships between location-based sex work types and the drugs used. One-third of the street sex workers reported regular amphetamine use, but none reported regular cocaine use. On the contrary, no escorts reported regular amphetamine use, but 38% admitted to regular cocaine use. The location of sex work may pose an additional occupational health risk factor for substance use. Regular use of alcohol was twice as typical (64%) for sex workers who were employed in bars, in salons/parlors, or alone in rented apartments than it was for those working in other indoor locations (33-34%). Furthermore, 74% of street sex workers smoked tobacco compared with 17% of escorts. Problem drug use was roughly estimated by asking the participants about the main problem domains (medical, legal, social, etc.) from the Addiction Severity Index instrument. The most problematic drug was amphetamine, and the most frequent problem was prolonged or excessive drug use. These main findings may contribute to more focused planning of health intervention services, harm reduction measures, outreach programs, and specific treatments.

Voice of the psychonauts: coping, life purpose, and spirituality in psychedelic drug users.

Psychoactive drug use shows great diversity, but due to a disproportionate focus on problematic drug use, predominant nonproblematic drug use remains an understudied phenomenon. Historic and anecdotal evidence shows that natural sources of "psychedelic" drugs (e.g., mescaline and psilocybin) have been used in religious and spiritual settings for centuries, as well as for psychological self-enhancement purposes. Our study assessed a total of 667 psychedelic drug users, other drug users, and drug nonusers by online questionnaires. Coping, life purpose, and spirituality were measured with the Psychological Immune Competence Inventory, the Purpose in Life test, and the Intrinsic Spirituality Scale, respectively. Results indicate that the use of psychedelic drugs with a purpose to enhance self-knowledge is less associated with problems, and correlates positively with coping and spirituality. Albeit the meaning of "spirituality" may be ambiguous, it seems that a spiritually-inclined attitude in drug use may act as a protective factor against drug-related problems. The autognostic use of psychedelic drugs may be thus hypothesized as a "training situation" that promotes self-enhancement by rehearsing personal coping strategies and by gaining self-knowledge. However, to assess the actual efficiency and the speculated long-term benefits of these deliberately provoked exceptional experiences, further qualitative investigations are needed.

[Education of surgeons at the Medical School of the University of Pest between 1825 and 1848]. / Sebészképzés a pesti egyetem Orvosi Karán a reformkorban.

From the 1820's a great number of surgeons attended the Faculty of Medicine in Pest. The increasing number of the students was resulted by several circumstances. After the Napoleonic wars peregrination from Hapsburg lands was prohibited. The Faculty of Medicine in Pest however offerred a diploma for its students valid for the whole territory of the Hapsburg Empire. The lectures were held in Hungarian or German, in contrary to the exclusively Latin lectures for medical students. A shorter preliminary education was needed. After three years the student could get his degree Chirurgiae Magister or Chirurgus civilis--the length of studies depended on the pleriminary studies of the surgeon. The Faculty started only two courses, so the surgeons mostly repeated the second or both courses. Although these degrees proved to be inferior to the titles Doctor Medicinae or Doctor Chirurgiae, many students wanted to get it. In the beginning of this period, in 1825/1825 117 persons attended the first semesters of the German, and 59 of the Hungarian course. This growth reached its peak in the years of the cholera. In 1832/1833 255 person matriculated to the first class of the German and 148 to the Hungarian course. This new contagion damaged not only human lifes, but the popularity of the surgical education as well. The number of students began slowly decrease. Another reason for this decrease was the new possibility for peregrination from the 1830's. In 1846/1847 48 persons matriculated to the first German course, and 49 to the Hungarian, while in the second one their number was only 10 and 20. As we can see, the German course was more popular, here came students from the other parts of the Hapsburg Empire, especially Jews: from Moravia (160; 110 of them was Jew), Bohemia (134) or Galicia (178; 124 of them was Jew). Between 1825 and 1846 2618 surgeons matriculated to the Faculty of Pest, many of them however left it with or without doing their exams. 1854 students took every exam, but only 259 got his degree as Chirurgiae Magister in Pest, and 1049 as Chirurgiae Civilis. Presumably many students carried on their studies in Vienna. Although the Faculty of Medicine in Pest was less renowned, than the medical faculty of Vienna or Prague, it definitely played a remarkable role in the contemporary medical and surgical education of the Hapsburg Empire.