ABSTRACT
The complement resistance of Aeromonas salmonicida strains grown under conditions promoting capsule formation was investigated using well characterized strains and their isogenic mutants. Complement resistance was previously studied using the same strains growing under non-capsulating conditions. The serum resistant strains were found to activate complement, but rapidly degrade C3b preventing productive formation of the lytic complex C5b-9. Isogenic lipopolysaccharide rough mutants grown under non-capsulating conditions were serum sensitive, binding a large amount of C3b and leading to productive formation of C5b-9. When grown under conditions promoting capsule formation, these mutants were partially resistant to complement because less C3b is bound to them and also partially degraded, with a concomitant reduction in lytic C5b-9.
Subject(s)
Aeromonas/immunology , Antibodies, Bacterial/immunology , Bacterial Capsules/immunology , Complement C3b/immunology , Complement C5/immunology , Animals , Complement C5b , Humans , Polysaccharides, Bacterial/immunology , RabbitsABSTRACT
The ability of several Aeromonas salmonicida strains grown under different conditions (capsulated and non-capsulated) to adhere to and invade two fish cell lines was compared. The level of adherence was slightly higher when the strains were grown under conditions promoting capsule formation than when the same strains were grown under conditions which did not promote capsule formation. However, the most significant difference among the wild-type strains grown under conditions promoting capsule formation was the ability to invade fish cell lines, which was significantly higher than when the same strains were grown under conditions which did not promote capsule formation. From these results we conclude that the capsular polysaccharide, in these strains, is an important factor for intracellular invasion.
Subject(s)
Aeromonas/chemistry , Bacterial Adhesion , Bacterial Capsules/physiology , Gram-Negative Bacterial Infections/microbiology , Polysaccharides, Bacterial/physiology , Aeromonas/immunology , Aeromonas/pathogenicity , Animals , Antibodies, Blocking/immunology , Bacterial Capsules/immunology , Bass , Carps , Cells, Cultured , Culture Media , Gram-Negative Bacterial Infections/immunology , Polysaccharides, Bacterial/immunologyABSTRACT
Mesophilic Aeromonas hydrophila from serotypes O:11 and O:34 grown in a glucose-rich medium produce a capsule that can be seen under light and electron microscopy. The purified capsular polysaccharide has a composition qualitatively similar for strains O:11 and O:34, but quantitatively different. The capsular polysaccharides were immunogenic in rabbits, and did not cross-react with specific antibodies against either purified lipopolysaccharide from strains O:34 or O:11 or against the S-layer characteristic of strains from serotype O:11.
Subject(s)
Aeromonas hydrophila/chemistry , Bacterial Capsules/chemistry , Aeromonas hydrophila/classification , Bacterial Capsules/immunology , Microscopy, ElectronABSTRACT
During batch aerobic submerged fermentation, the exopolysaccharide synthesis by Pseudomonas sp. strain EPS-5028 occurred in growth- and non-growth-linked processes. Polysaccharide formation increased when the pH was controlled at 7 during fermentation. Exopolysaccharide production depended on the phosphate content of the medium. The polymer exhibited a pseudoplastic nature, had good thermostability, and was affected neither by pH nor by high concentrations of salt.
ABSTRACT
Dimethoate and malathion added to soil at 10 and 100 microgram/g caused an initial stimulation of CO2 production. Total counts of bacterial propagules were increased. All insecticide applications increased bacteria producing phospholipases from week 1 until week 4 after the application; bacteria then returned to the original levels.
