[The effect of electromagnetic field on the biosynthesis and various properties of extracellular proteinase and lectin from Bacillus subtilis 316 M]. / Vliianie élektromagnitnogo polia na biosintez i nekotorye svoistva vnekletochnoi proteinazy i lektina Bacillus subtils 316 M.

The promoting effect of electromagnetic field (EMF) on biosynthesis and activity of extracellular proteinase and lectin in B. subtilis 316 M was observed. It caused 1.5- and 4-fold increase of the metabolites yield respectively. The EMF stimulated a 2-fold activation of lectin, rise of the enzyme activity and a shift of it pI from 11.4-11.5 to 9.2-9.3.

[Problems in the diagnosis and clinical assessment of atrial dissociation (a report on 2 cases and review of the literature)]. / Voprosy diagnostiki i klinicheskoi otsenki predserdnoi dissotsiatsii (soobshchenie o dvukh sluchaiakh nabliudeniia i obzor literatury).

At registration of ECG esophageal leads in two patients with ischemic heart disease an extra atrial rhythm was recorded missed by standard ECG. Similar cases from the literature are considered.

[The purification and properties of an extracellular sialo-specific lectin of Bacillus subtilis 316M]. / Ochistka i nekotorye svoistva vnekletochnogo sialospetsifichnogo lektina Bacillus subtilis 316M.

A simple procedure is proposed for purification of lectin from the culture liquid of non-pathogenic Bacillus subtilis 316M, which includes fractionation with ammonium sulfate and rechromatography on Sepharose CL-6B. The procedure enables a 213-fold purification of lectin with a specific activity of 2560 U/mg protein and 51% recovery in activity. According to gel-filtration through Sepharose the lectin has a molecular weight of 190 kDa. It consists of two types of subunits with hemagglutinating activity. The lectin is highly specific to N-glycolylneuraminic and N-acetylneuraminic acids and fructose 1.6-biphosphate.

[Optimization of the nutrient medium composition for directed biosynthesis of Bacillus mesentericus lectins]. / Optimizatsiia sostava pitatel'noi sredy dlia napravlennogo biosinteza lektinov Bacillus mesentericus.

The medium has been optimized in order to increase the lectin-producing activity of Bacillus mesentericus. When a producer grew on the optimized medium, a 4-8-fold increase of the hemagglutination test titres and a 5.6-fold increase (as compared with the initial medium) of the specific lectin activity of the culture liquid was attained.