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Vopr Virusol ; 49(6): 34-7, 2004.
Article in Russian | MEDLINE | ID: mdl-15597959

ABSTRACT

Pulmonary tissues of red-grey voles, Clethrionomys rufocanus from Shkotovo District and Maritime Territory were investigated. rt-PCR was used to detect the hantavirus nt-222 M-segment genome and nt-403 mitochondrial DNA fragment. A new genetic variant of the PUUV virus named as "Shkotovo", that is different from other PUUV strains by 18-23%, was shown to be circulating in red-grey voles. A phylogenetic analysis denoted 2 PUUV subgroups with their rodent-host branching into C. graleolus and C. rufocanus.


Subject(s)
Arvicolinae/virology , Genome, Viral , Lung/virology , Puumala virus/genetics , Amino Acid Sequence , Animals , DNA, Mitochondrial/analysis , Genetic Variation , Mice , Molecular Sequence Data , Phylogeny , Puumala virus/isolation & purification , RNA, Viral/analysis , Reverse Transcriptase Polymerase Chain Reaction , Sequence Alignment , Siberia
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