ABSTRACT
AIM: To study features of antigen-antibody interaction during use of linear synthetic peptides and multipeptide antigen modelling antigenic determinants of hepatitis A virus (HAV) and to evaluate perspectives for use of heterogeneous tetrameric multipeptide antigens for detection of HAV serological markers. MATERIALS AND METHODS: Linear peptides VP1 and VP3 were synthesized by fluorenylmethyloxycarbonyl (Fmoc)-polyamide solid phase method. MAP4 (VP1+VP3) was synthesized according to 9-Fmoc strategy. Interaction of these peptides with anti-HAV IgM positive sera from patients with HA was studied by noncompetitive and competitive methods of immunoenzyme assay. RESULTS. Using immunoenzyme assay, high heterogeneity of immune response in patients with HA (62 and 67% in two groups) was shown. MAP4 (VP1+VP3), unlike the combination of linear peptides VP1 and VP3, interacted with anti-HAV IgM in 41 - 45% of sera and, at the same time, did not lead to false positive results. CONCLUSION: Population of HAV is not so uniform which is usually assumed. It could be reasonable to use heterogenous multipeptide antigens, including those containing VP1 (11 - 25 a.r.) and VP3 (110 - 121 a.r.), for the development of new assays for HA diagnostics.
Subject(s)
Capsid Proteins/immunology , Epitopes/immunology , Hepatitis A Antibodies/immunology , Hepatitis A Antigens/immunology , Hepatitis A Virus, Human/immunology , Hepatitis A/immunology , Peptide Fragments/immunology , Viral Structural Proteins/immunology , Antibody Specificity , Antigen-Antibody Reactions , Biomarkers , Capsid Proteins/chemical synthesis , Hepatitis A/diagnosis , Humans , Immunoglobulin M/immunology , Peptide Fragments/chemical synthesis , Viral Structural Proteins/chemical synthesisABSTRACT
The results of examinations of 35 patients aged 17 to 42 years who had abnormalities in the cervix uteri were analyzed. Colposcopy detected an atypical epithelium in 100% of cases. To verify the diagnosis, biopsy specimens from the cervix uteri were morphologically studied prior to and following treatment. Before treatment, there was a slight proliferative activity of the epithelium in 45% of cases and its high proliferative activity in 15% in uncomplicated papilloma viral infection (PVI). A follow-up of complicated PVI indicated increases in the expression of a proliferative nuclear antigen (PVA) with higher dysplasia: a moderate response in 75% of D3 + PVI cases and a higher response in 60% of preinvasive cancers. Analysis of PCNA-immunological reactivity of biopsy specimens taken after treatment estimates the efficiency of the chosen complex of treatments: there was a negative result of immunohistochemical responses.
Subject(s)
Cervix Uteri/pathology , Condylomata Acuminata/diagnosis , Papillomaviridae , Papillomavirus Infections/diagnosis , Tumor Virus Infections/diagnosis , Uterine Cervical Diseases/diagnosis , Adolescent , Adult , Biopsy , Cell Division , Colposcopy , Condylomata Acuminata/therapy , Epithelial Cells/pathology , Female , Humans , Immunohistochemistry , Papillomavirus Infections/pathology , Papillomavirus Infections/therapy , Precancerous Conditions/diagnosis , Tumor Virus Infections/pathology , Tumor Virus Infections/therapy , Uterine Cervical Diseases/therapy , Uterine Cervical Neoplasms/diagnosisABSTRACT
Monoclonal antibodies PC-10 were used to study the expression of PCNA in precancerous pathology of the uterine cervix in 50 biopsies in the presence of papilloma virus infection (PVI). Positive reaction was in 100% PVI before treatment; weak proliferative activity was in 40% and strong reaction in 15%. If PVI was complicated with degree III dysplasia, there was an increase of proliferative activity. The positive reaction was retained after the therapy in 4 cases of the recurrent PVI. Positivity with PC-10 may be an unfavourable prognostic criterium.
