ABSTRACT
Nutritional stress disturbs the cellular redox-status, which is characterized by the increased generation of reactive oxygen species (ROS). The NRF2-NQO1 axis represents a protective mechanism against ROS. Its strength is cell type-specific. FaDu, Cal 27 and Detroit 562 cells differ with respect to basal NQO1 activity. These cells were grown for 48 hours in nutritional conditions (NC): (a) Low glucose-NC2, (b) no glucose, no glutamine-NC3, (c) no glucose with glutamine-NC4. After determining the viability, proliferation and ROS generation, NC2 and NC3 were chosen for further exploration. These conditions were also applied to IMR-90 fibroblasts. The transcripts/transcript variants of NRF2 and NQO1 were quantified and transcript variants were characterized. The proteins (NRF2, NQO1 and TP53) were analyzed by a western blot in both cellular fractions. Under NC2, the NRF2-NQO1 axis did not appear activated in the cancer cell lines. Under NC3, the NRF2-NQO1axis appeared slightly activated in Detroit 562. There are opposite trends with respect to TP53 nuclear signal when comparing Cal 27 and Detroit 562 to FaDu, under NC2 and NC3. The strong activation of the NRF2-NQO1 axis in IMR-90 resulted in an increased expression of catalytically deficient NQO1, due to NQO1*2/*2 polymorphism (rs1800566). The presented results call for a comprehensive exploration of the stress response in complex biological systems.
Subject(s)
Glucose/deficiency , Glutamine/deficiency , Head and Neck Neoplasms/metabolism , NAD(P)H Dehydrogenase (Quinone)/metabolism , NF-E2-Related Factor 2/metabolism , Oxidative Stress , Cell Culture Techniques/methods , Cell Line , Head and Neck Neoplasms/pathology , Humans , Reactive Oxygen Species/metabolismABSTRACT
Human Body Communication (HBC) is an attractive low complexity technology with promising applications in wearable biomedical sensors. In this paper, a simple parametric model based on the finite-element method (FEM) using a full human body model is developed to virtually emulate and examine the HBC channel. FEM allows better modeling and quantification of the underlying physical phenomena including the impact of the human body for the desired applications. By adjusting the parameters of the model, a good match with the limited measurement results in the literature is observed. Having a flexible and customizable simulation platform could be very helpful to better understand the communication medium for capacitively coupled electrodes in HBC. This knowledge, in turn, leads to better transceiver design for given applications. The platform presented here can also be extended to study communication channel characteristics when the HBC mechanism is used by an implant device.
Subject(s)
Computer Simulation , Electrodes , Wearable Electronic Devices , Wireless Technology , Finite Element Analysis , Human Body , HumansABSTRACT
Europe is the only region in the world with common legislative acts regulating exposure to electromagnetic fields (EMF) for both the general public and workers. Council Recommendation 1999/519/EC deals with the limitation of exposure of the general public to EMF (0 Hz-300 GHz). Directive 2004/40/EC regulates the minimum health and safety requirements regarding the exposure of workers to the risks arising from EMF. This paper discusses the general application of existing standards and recommendations in measurement techniques for determining compliance of measured exposure limit values and action values with those defined in 2004/40/EC.
Subject(s)
Computer Communication Networks/instrumentation , Electromagnetic Fields/adverse effects , Guideline Adherence , Occupational Exposure/prevention & control , Radiation Monitoring/methods , Europe , European Union , Guidelines as Topic , Humans , Occupational Exposure/legislation & jurisprudence , Occupational Exposure/standards , Telecommunications/instrumentationABSTRACT
The rapid growth of mobile communications has not only led to a rising number of mobile telephones. It has also made base stations essential for these services widespread on many roofs. However, not everyone is aware that working close to sources of high frequency electromagnetic fields (EMF), such as transmitter antennas for mobile phones, pagers and police, fire and other emergency services, can result in high EMF exposure. This paper deals with measurements and calculations of the compliance boundary for workers in one typical roof top base station setting according to EU Directive and other relevant EN standards.
Subject(s)
Electromagnetic Fields/adverse effects , Guideline Adherence , Occupational Exposure/prevention & control , Computer Communication Networks/instrumentation , Europe , European Union , Government Regulation , Guidelines as Topic , Humans , Maintenance , Occupational Exposure/analysis , Occupational Exposure/legislation & jurisprudence , Radiation Monitoring , Telecommunications/instrumentationABSTRACT
This paper gives an overview of the regulatory acts in non-ionising radiation in the world, with a special emphasis on basic guidelines issued by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). ICNIRP Guidelines are implemented in many countries worldwide. Croatia has also implemented them indirectly through the European Recommendation 1999/519/EC. The Croatian regulatory acts include the Non-lonising Radiation Protection Act, Ordinance on Electromagnetic Fields (EMF) Protection, and the Ordinance on Basic Requirements for Devices which produce Optical Radiation and Measures for Optical Radiation Protection. Dosimetry and densitometry are compliant with relevant international and European standards. The paper presents an example of densitometric human exposure assessment in complex indoor exposure conditions. In spite of a high number of indoor and outdoor sources and the "worst-case exposure assessment", the results are within the limits defined by the Croatian EMF Ordinance.
