[Cellular immunity factors in salmonellosis and food toxinfections]. / Sostoianie faktorov kletochnogo immuniteta pri sal'monelleze i pishchevykh toksikoinfektsiiakh.

A complex immunological examination of 68 salmonellosis patients and 227 patients with alimentary toxinfections of unknown etiology has been made in the process of their treatment by rehydration therapy. At the acute period of the disease, irrespective of its nosologic form, cellular immunity has been suppressed. Cell-mediated immunity factors have been shown to depend on the severity of the disease.

[Disorders in the thrombocyte link of hemostasis during endotoxinemia and their correction with the inhibitor of prostaglandin biosynthesis, indomethacin]. / Narusheniia v trombotsitarnom zvene gemostaza pri éndotoksinemii i korrektsiia ikh ingibitorom biosinteza prostaglandinov, indometatsinom.

After 10 minutes of endotoxin Salmonella typhimurium (1 mg/kg) injection into rabbits thrombocytopenia appeared, the aggregation and secretory function of circulating platelets reduced, the transformation of platelet forms from disk-shaped into spheroidal took place. On the surface of plasmatic membranes the pseudopodies and aggregates examining samples of PRP were observed. Indomethacin, blockator of biosynthesis of prostaglandins results in normalisation of morphofunctional properties of platelets.

[Ultrastructural study of the surface formations of Salmonella typhimurium H-3048 grown on a medium with sodium arachidonate added]. / Izuchenie ul'trastruktury poverkhnostnykh obrazovanii Salmonella typhimurium H-3048, vyrashchennoi na srede s dobavleniem natrievoi soli arakhidonovoi kisloty.

Ultrastructural changes in the surface formations of S. typhimurium grown in a culture medium with sodium arachidonate added at different concentrations have been studied by means of electron microscopy.

[Effect of a live enteral dysentery vaccine from spontaneous Flexner mutant 2a 516m on the immunoglobulin-producing cell count in the large intestine mucosa in dysentery]. / Vliianie zhivoi dizenteriinoi enteral'noi vaktsiny iz spontannogo mutanta Fleksnera 2a 516m na soderzhanie immunoglobulinprodutsiruiushchikh kletok v slizistoi tolstoi kishke bol'nykh dizenteriei.

The influence of vaccinal therapy with live oral dysentery vaccine prepared from S. flexneri 2a 516M on the content of immunoglobulin-producing cells in the mucous membrane of the large intestine was studied. A considerable increase in the number of IgA- and IgM-synthetizing cells was shown to occur in the course of the infectious process in acute dysentery. In chronic dysentery the content of IgA- and IgM-synthetizing cells in patients was considerably lower than in a smooth course of acute dysentery. The use of the vaccine for the therapy of patients with chronic and especially acute dysentery resulted in a considerable rise in the number of plasma cells synthetizing IgA, IgM and IgG.

[Comparative study of modifications in the immunoperoxidase and immunofluorescent methods of detecting human tissue immunoglobulins]. / Sravnitel'noe izuchenie modifikatsii immunoperoksidaznogo i immunofliuorestsentnogo metodov vyiavleniia tkanevykh immunoglobulinov cheloveka.

The comparison of the data obtained by using different modifications of the immunoperoxidase and immunofluorescent methods for detecting immunoglobulins in intestinal tissues was made. The direct immunoperoxidase method was shown to allow the quantitative study of immunoglobulin-containing cells. The direct immunoperidase method was found to be technically simpler, but the indirect and complex peroxidase-antiperoxidase methods proved to be more sensitive.

[Quantitative characteristics of the immunocompetent cells of the large-intestinal mucosa in acute dysentery]. / Kolichestvennaia kharakteristika immunokompetentnykh kletok slizistoi obolochki tolstoi kishki pri ostroi dizenterii.

Histological and immuno-morphological examinations of the large intestine mucosa obtained by aspirational biopsy from 38 adult patients with acute dysentery in the mild, moderately severe and severe forms and from 4 normal subjects were carried out. In each case, a per cent ratio of immunocompetent cells in deep and superficial parts of the mucous membrane proper, the content of interepithelial lymphocytes, the cell density of infiltrate per 1 mm2 of the mucous membrane, the number of cells producing IgA, IgM, and IgG per 1 mm2 of the mucous membrane were determined. As the severity of clinical and morphological manifestations of dysentery increased, the number of cells actively producing immunoglobulins was found to increase and epithelium infiltration with lymphocyte to be enhanced. The main role in the development of local immune response in dysentery is played by IgA-producing cells, and in the moderately severe and severe forms of dysentery the number of IgM-producing cells increases.

[Intraepithelial shigellae invasion of the intestinal mucosa in patients suffering from acute Sonne dysentery]. / O vnutriépitelial'noi invazii shigell v slizistoi obolochke kishechnika u bol'nykh ostroi dizenteriei Zonne

Data are presented on detection of intraepithelial localization of shigellae in the biopsy materials of the large and small intestine mucosa of the patients with various forms of acute Sonne dysentery, in comparison with the clinico-morphological picture of the disease. Biopsy specimens (60 of the large and 7 of the small intestine) were stained with thionine and examined bacterioscopically; an intracellular distribution of the microbes was revealed in 58 cases; in 53 of them a subsequent identification of the microbes by the fluorescent antibody method was carried out; in 47 cases (88.7%) invasion of Sh. sonnei into the cells of the epithelium of the mucosa of the large and the small intestine was confirmed. There proved to be a direct relationship between the severity of the course of the disease, the level of shigellae localization and the extent of contamination by them of the cells of the large intestinal mucosa. The role of the penetrating capacity of shigellae in the development of the pathological process in dysentery is discussed.