The reliability of the angle of deviation measurement from the Photo-Hirschberg tests and Krimsky tests.

OBJECTIVE: To compare the angle of deviation measured from Photo-Hirschberg testing and Krimsky testing, with that from an alternate prism cover test (APCT) in strabismus patients. METHODS: A cross-sectional study was conducted in Songklanagarind Hospital, Thailand. Thirty-three strabismus patients were photographed for analysis by Photo-Hirschberg testing using computer software. The corneal light reflex displacement, converted into prism diopter (PD), was compared to the angle of deviation measured with APCT. Twenty-eight strabismus patients were tested with the Krimsky test. Data were analyzed using Pearson correlation and paired t-tests. The study excluded 4 intermittent exotropia cases, 1 intermittent esotropia case and 2 which cases missing data for krimsky test. RESULTS: The mean±SD of the deviation angle, measured by APCT with a fixation target at 30 cm and 6 m; were 48.09±16.34PD and 47.82±15.73 PD, respectively. At 1 m, the difference in the angle of deviation measured from APCT and the Photo-Hirschberg test within 10 PD were 58.8% and 63.6%, for ET and XT, respectively. The difference in the angle of deviation measured from APCT and Krimsky tests within 10 PD in ET and XT were 86.7% and 80.0%, respectively. At 4 m, the difference in angle of deviation measured from APCT and Photo-Hirschberg tests within 10 PD in ET and XT were 58.8% and 54.5%, respectively; whereas, the difference in the angle of deviation measured from APCT and Krimsky tests within 10 PD in ET and XT were 80.0% and 70.0%, respectively. CONCLUSION: The reliability of Krimsky test was better than Photo-Hirschberg test for measuring an angle of deviation.

Distribution of Class II integrons and their contribution to antibiotic resistance within Enterobacteriaceae family in India.

BACKGROUND: Integrons are the main contributors to the development of multidrug resistance (MDR) among Gram-negative bacilli. There is a lack of knowledge about the molecular relation between gene cassettes and antibiotic resistance in India. OBJECTIVE: In this study, we have investigated the occurrence of Class II integron and their cassette array among Enterobacteriaceae. MATERIALS AND METHODS: A total of 268 MDR non-duplicate strains of Enterobacteriaceae were collected from Silchar Medical College and Hospital, Silchar, Assam, India, during June 2012 to May 2013. Polymerase chain reaction was performed for detection of the integrase genes and gene cassettes within the Class II integron which were further analysed by sequencing. RESULTS: Class II integron was observed in 47 isolates. Four different gene cassette arrangements were detected: dfrA1-sat2-aadA1; dfrA1-sat2-aadA1-orfX-ybeA-ybfA-ybfB-ybgA; dfrA12-sat2-aadA1; and dfrA1-linF-aadA1. The most prevalent cassette combination was dfrA1-sat2-aadA1. This study has also identified a set of gene cassette associated with linF gene instead of sat2 gene. CONCLUSION: Further investigation is required to determine the current situation and important reservoir of Class II integron for the transmission of drug resistance among Enterobacteriaceae and their contribution to antimicrobial resistance in hospital environment .

Manumycin A inhibits triple-negative breast cancer growth through LC3-mediated cytoplasmic vacuolation death.

Therapy resistance can be attributed to acquisition of anti-apoptotic mechanisms by the cancer cells. Therefore, developing approaches that trigger non-apoptotic cell death in cancer cells to compensate for apoptosis resistance will help to treat cancer effectively. Triple-negative breast cancers (TNBC) are among the most aggressive and therapy resistant to breast tumors. Here we report that manumycin A (Man A), an inhibitor of farnesyl protein transferase, reduces cancer cell viability through induction of non-apoptotic, non-autophagic cytoplasmic vacuolation death in TNBC cells. Man A persistently induced cytoplasmic vacuolation and cell death through the expression of microtubule-associated protein 1 light chain 3 (LC3) and p62 proteins along with endoplasmic reticulum (ER) stress markers, Bip and CHOP, and accumulation of ubiquitinated proteins. As inhibitors of apoptosis and autophagy failed to block cytoplasmic vacuolation and its associated protein expression or cell death, it appears that these processes are not involved in the death induced by Man A. Ability of thiol antioxidant, NAC in blocking Man A-induced vacuolation, death and its related protein expression suggests that sulfhydryl homeostasis may be the target of Man A. Surprisingly, normal human mammary epithelial cells failed to undergo cytoplasmic vacuolation and cell death, and grew normally in presence of Man A. In conjunction with its in vitro effects, Man A also reduced tumor burden in vivo in xenograft models that showed extensive cytoplasmic vacuoles and condensed nuclei with remarkable increase in the vacuolation-associated protein expression together with increase of p21, p27, PTEN and decrease of pAkt. Interestingly, Man A-mediated upregulation of p21, p27 and PTEN and downregulation of pAkt and tumor growth suppression were also mimicked by LC3 knockdown in MDA-MB-231 cells. Overall, these results suggest novel therapeutic actions by Man A through the induction of non-apoptotic and non-autophagic cytoplasmic vacuolation death by probably affecting ER stress, LC3 and p62 pathways in TNBC but not in normal mammary epithelial cells.

Mortality and cause of death in abuth, zaria: 1999 - 2005.

BACKGROUND: Accurate mortality statistics are needed for policy formulation, implementation and monitoring of health intervention that are aimed at improving the health status of the people. Mortality level is one of the indicators of the quality of life and status of health of a population. However, accurate collection, collation, analysis and interpretation of such data is poorly organised in developing nations, including Nigeria leading to a gap in health policy formulation, implementation and monitoring. Therefore, policies and strategies for disease prevention are based on empirical evidence rather than on data primarily collected to formulate disease specific interventions. Though, hospital data have inherent deficiency in its use to design prevention. However, when accurately generated and adequately managed would provide both qualitative and quantitative information on morbidity and mortality if not for the entire society at least for a segment of the population utilizing it. We implemented a system of death certification to determine causes and pattern of mortality in Ahmadu Bello University Teaching Hospital, Zaria. METHODS: From May 1999 to November 2005, all case folders of deceased patients were retrieved from the central library of health information management department of the hospital; case folders of deceased patients are required to have in them a completed IFMCCD (International Form of Medical Certification of Cause of Death). All case folders of deceased patients after relevant information were extracted by the staff of health management information department, were passed on to the staff of department of Community Medicine directly involved in this study. The completed cause of death certificates received in the department of Community Medicine (between May 1999 and November 2005), were examined. Coding rules were employed to select the appropriate code for those certificates that were incorrectly completed. The underlying cause of death as identified from the correctly completed IFMCCDS is coded according to ICD-10. RESULTS: For the period under study, there were 4019 deaths: 2212 males and 1807 females. Total of 2914 (72.5%) deaths were certified, using the IFMCCD of which 1641 of them were males and 1273 females and formed the basis of this analysis. Coverage rates ranges from 56.2% in 2001 to 85% in 1999. The proportion of garbage codes ranges from 0% to 2.4% while the three leading causes of death are HIV infection, road traffic accident (RTA), and cardiovascular diseases among the ten. The time-trend of the leading causes of death show RTA maintaining steady upward climb while malaria, septicemia, PEM, sepsis in the neonatal period shows unsteady fluctuation. CONCLUSION: This study assessed the pattern of mortality and causes of death in ABU Teaching Hospital, Zaria; it also provided information on leading causes of death.

Mortality and Cause of Death in Abuth; Zaria: 1999-2005

Background: Accurate mortality statistics are needed for policy formulation; implementation and monitoring of health intervention that are aimed at improving the health status of the people. Mortality level is one of the indicators of the quality of life and status of health of a population. However; accurate collection; collation; analysis and interpretation of such data is poorly organised in developing nations; including Nigeria leading to a gap in health policy formulation; implementation and monitoring. Therefore; policies and strategies for disease prevention are based on empirical evidence rather than on data primarily collected to formulate disease specific interventions.Though; hospital data have inherent deficiency in its use to design prevention. However; when accurately generated and adequately managed would provide both qualitative and quantitative information on morbidity and mortality if not for the entire society at least for a segment of the population utilizing it. We implemented a system of death certification to determine causes and pattern of mortality in Ahmadu Bello University Teaching Hospital; Zaria Methods: From May 1999 to November 2005; all case folders of deceased patients were retrieved from the central library of health information management department of the hospital; case folders of deceased patients are required to have in them a completed IFMCCD(International Form of Medical Certification of Cause of Death). All case folders of deceased patients after relevant information were extracted by the staff of health management information department; were passed on to the staff of department of Community Medicine directly involved in this study. The completed cause of death certificates received in the department of Community Medicine (between May 1999 and November 2005); were examined. Coding rules were employed to select the appropriate code for those certificates that were incorrectly completed. The underlying cause of death as identified from the correctly completed IFMCCDS is coded according to ICD-10. Results: For the period under study; there were 4019 deaths: 2212 males and 1807 females. Total of 2914 (72.5) deaths were certified; using the IFMCCD of which 1641 of them were males and 1273 females and formed the basis of this analysis. Coverage rates ranges from 56.2in 2001 to 85in 1999. The proportion of garbage codes ranges from 0to 2.4while the three leading causes of death are HIV infection; road traffic accident (RTA); and cardiovascular diseases among the ten. The time-trend of the leading causes of death show RTA maintaining steady upward climb while malaria; septicemia; PEM; sepsis in the neonatal period shows unsteady fluctuation. Conclusion: This study assessed the pattern of mortality and causes of death in ABU Teaching Hospital; Zaria; it also provided information on leading causes of death

A novel role for MAP1 LC3 in nonautophagic cytoplasmic vacuolation death of cancer cells.

Thiol reactive cyclopentenone prostaglandin, 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ2), induced a novel, nonapoptotic and microtubule-associated protein 1 light chain 3 (MAP1 LC3) dependent but nonautophagic form of cell death in colon, breast and prostate cancer cell lines, characterized by extensive cytoplasmic vacuolation with dilatation of endoplasmic reticulum (ER). Disruption of sulfhydryl homeostasis, which resulted in ER stress, accumulation of ubiquitinated proteins and subsequent ER dilation, contributed to peroxisome proliferator-activated receptor gamma (PPARgamma)-independent cell death by 15d-PGJ2. Absence of intracellular organelles in these vacuoles, shown by electron microscopy and unique fragmentation of lamin B, suggested this form of cell death to be different from autophagy and apoptosis. Cell death induced by 15d-PGJ2 is prevented by cycloheximide and actinomycin D, suggesting a requirement of new protein synthesis for death with cytoplasmic vacuolation. Here, we report for the first time that upregulation and processing of autophagy marker LC3 is an important event in nonautophagic cytoplasmic vacuolation and cell death. Notably, knockdown of LC3 conferred significant protection against 15d-PGJ2-induced cytoplasmic vacuolation and cell death, suggesting a novel role of LC3 in a death process other than autophagy.

Evaluation of Occupational Health Services and Hazards in a Car Assembly Plant in Kaduna; Nigeria

Background: The provision of occupational health services in most industries of the developing world has been very rudimentary. Various factors have been adduced to be responsible for this, none availability of protective devices, non-compliance by workers and managerial problems among others. Therefore this study was conducted to evaluate occupational hazards and the provision of occupational health services at Peugeot Automobile Nigeria (PAN), Kaduna.Aim: To assess the extent of occupational hazards and the occupational health services provided by the industry (PAN). Methods: This was a cross sectional descriptive study in which cluster sampling technique was used to sample respondents. Structured, interviewer administered questionnaires with close ended questions were used to collect data.Results: One hundred and ten respondents participated in the study with a mean age of 32.7±2.8 years. Use of protective devices by respondents was: boots 96.3%, hand gloves 100%, and ear plugs 58% respectively. Pattern of occupational hazards were: exposure to chemical fumes 6.4%, noise pollution 40.9%, and chemical burns 9.1% respectively. Conclusion: Occupational hazards and injuries among PAN workers are a frequent occurrence. There is need for sustained public awareness campaigns among the workers and management on the importance of occupational safety to guide against injuries/ accidents at work sites and compliance to the use of protective devices. It is also recommended that the Inspectorate Division of Federal Ministry of Industries should pay periodic monitoring visits to industries in order to ensure a conducive and a safe working environment and also with the facility Act and related laws so as to curtail the rate of accidents and injures in Nigerian industries

Fertility pattern of Muslim Hausa women in northern Nigeria.

PIP: This paper reports the results of a fertility survey conducted in 1980 in Malumfashi town in northern Nigeria. The study covered 302 Hausa-speaking households in 2 wards. Of the 302 married males surveyed, 168 were monogamous, 92 had 2 wives, 30 had 3 wives, and 12 and 4 wives (the maximum allowable). All 168 monogramous marriages were not monogamous at the start. The average duration of present marriage among respondents was 14.1 years for monogamous unions and 11.0 years for polygymous unions. The average number of children ever born to women over 45 years of age was 6.3 (6.7 in monogamous unions and 6.0 in polygamous unions). The average family size for all marriages was 3.6, but this value was 4.6 in monogamous unions and 3.4 in polygamous unions (age standardized values were 4.5 and 3.4, respectively). There were 80 live births in the survey sample during the 12 months preceding the study, with a mean age at childbirth of 28.7 years. The crude birth rate for the surveyed population was 48.06/1000 (46/1000 for monogamous families and 43/1000 for polygamous families). The infant mortality rate was 137.5/1000 live births. About 11% of women in the sample were sterile. The fertility rate observed in this study is lower than that in Nigeria as a whole, perhaps because of the prevalence of polygamous unions in this region. These results support the observation that women in polygamous unions have lower fertility than those in monogamous unions.^ieng

Evaluation of the diethylcarbamazine provocative test in the diagnosis of Wuchereria bancrofti infections in the Nigerian savanna and the effects on Dipetalonema perstans.

Large scale filariasis surveys in rural areas for microfilaraemia, especially of periodic types such as Wuchereria bancrofti are known to cause considerable administrative, technical and social problems. The present investigation was carried out in the population of two villages in the Malumfashi district of the Northern Nigerian savanna. From the survey results, the sensitivity and specificity of two techniques-day-time diethylcarbamazine (DEC) provocative test by blood smear and concentration, and night-blood examination by smear and concentration especially for W. bancrofti-were assessed. Day-time DEC provocative test proved to be efficient in terms of sensitivity and specificity, compared with the night-blood method, for W. bancrofti detection but less so for Dipetalonema perstans, the other blood microfilaria found in this population during these studies. A regression line between night-blood survey results for W. bancrofti and the results from day-time DEC provocative test was calculated. With the help of this regression line it is possible to estimate W. bancrofti microfilarial prevalence for night surveys, using the DEC provocative test results of day-time surveys. This can be done with minimal, but known, loss of accuracy and incurs fewer administrative, technical and social difficulties.

Effectiveness of oral killed typhoid vaccine.

A controlled field trial of oral killed typhoid vaccine was carried out in Delhi, India in 1968-69. Altogether, 13 374 children below the age of 17 years were included in the study. Two comparable groups of children were given either placebo or vaccine. Each tablet of vaccine contained 100x10(9) killed Salmonella typhi, and 3 tablets of vaccine or placebo were administered to each child. The vaccinated subjects were followed up from 10 June 1968 to 31 August 1969. The effectiveness of the vaccine was measured by comparing the incidence of typhoid fever (based only on bacteriologically positive cases) in the two groups. It was found that the difference in incidence of the disease in the two groups was not statistically significant. The oral killed typhoid vaccine in the dosage schedule used in the present trial was found not to be effective against the disease.