ABSTRACT
The oral administration of amikacin, ampiox, nystatin to CBA mice and the external treatment of the animals with 1% chlorhexidine solution makes it possible to create the germ-free state in the animals which must be then kept in a sterile box. If such animals receive the decantate of the patient's feces, introduced in a single administration, the microflora, which is subsequently formed in the intestine of the recipient animals, is identical to the donor's microflora. This permits the rapid and accurate determination of the sensitivity of the patient's intestinal microflora to different antimicrobial preparations and their combinations. The antibacterial preparations, effectively suppressing the patient's intestinal anaerobic opportunistic microflora in the intestine of the recipient mice, produce, if subsequently prescribed for treatment, a pronounced corrective effect on such microflora in the patient's intestine.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Bacteria/drug effects , Intestines/microbiology , Adolescent , Animals , Anti-Bacterial Agents/pharmacology , Bacteria/isolation & purification , Bacterial Infections/drug therapy , Bacterial Infections/microbiology , Child , Decontamination/methods , Drug Therapy, Combination , Feces/microbiology , Germ-Free Life/drug effects , Humans , Intestinal Diseases/drug therapy , Intestinal Diseases/microbiology , Mice , Mice, Inbred CBA , Microbial Sensitivity Tests/methods , Time FactorsABSTRACT
The oral administration of kanamycin (40 mg/kg) or ampiox (500 mg/kg) to guinea pigs for 5 days led to disturbances in their normal intestinal microflora, manifested by a sharp decrease in the levels of lactobacteria and bifidobacteria, as well as by the appearance of large amounts of enterobacteria and enterococci, normally not detected in the proximal and distal sections of the intestinal tract. In adult volunteers receiving kanamycin orally in a dose of 40 mg/kg for 5 days disturbances in microbiocenosis also occurred: the amount of enterococci, staphylococci, lactobacteria and bifidobacteria considerably decreased, enterobacteria becoming the dominating microorganisms. Three oral administrations of bifidobacterial and lactobacterial autostrains immediately after the abolition of the antibiotic facilitated the rapid and effective restoration of the intestinal microflora.
