Subject(s)
Abnormalities, Multiple/genetics , Genetic Predisposition to Disease/genetics , Intellectual Disability/pathology , Seizures/pathology , Abnormalities, Multiple/pathology , Amino Acid Sequence , Base Sequence , Chromosomes, Human, X/genetics , DNA/chemistry , DNA/genetics , DNA Mutational Analysis , Ear/abnormalities , Family Health , Female , Genetic Linkage , Gynecomastia/pathology , Humans , Hypogonadism/pathology , Intellectual Disability/genetics , Male , Molecular Sequence Data , Obesity/pathology , Pedigree , Sequence Deletion , Sequence Homology, Amino Acid , SyndromeABSTRACT
The position of chromosomes 1, 9, and 16 in first and third mitoses of lymphocyte cultures was measured in BUdR-labelled air-dried chromosome preparations. No significant deviation from a random distance could be found between the two homologues of each chromosome, either in the first or in the third mitoses after phytohemagglutinin stimulation. Colchicine treatment also had no influence.
Subject(s)
Chromosomes, Human, 1-3/ultrastructure , Chromosomes, Human, 16-18/ultrastructure , Chromosomes, Human, 6-12 and X/ultrastructure , Adult , Bromodeoxyuridine , Cells, Cultured , Colchicine/pharmacology , Humans , Lymphocytes/ultrastructure , Male , Middle Aged , Mitosis , Phytohemagglutinins/pharmacology , Time FactorsABSTRACT
Chromosome aberrations in human lymphocytes in vitro and in vivo were studied after the treatment with the five-functional alkylating agent photrine (an original Soviet antitumour drug). In concentrations above 10-7 M photrine caused chromosome breaks in vitro at 4.6.10-4 M completely diminished the mitotic activity in cultured cells. The aberrations were presented almost exclusively as single and paired fragments occasionally distributed among the chromosomes. In three patients treated with photrine cells with aberrative karyotypes ranged within 14-37.5%.
