Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 6 de 6
Filter
Add more filters










Database
Language
Publication year range
1.
Adv Physiol Educ ; 45(1): 71-76, 2021 Mar 01.
Article in English | MEDLINE | ID: mdl-33529142

ABSTRACT

The amalgamation of educational technologies in higher education is widespread and has become essential for contemporary practice on a large scale. Foundational anatomy and physiology knowledge is integral to all courses in health education. To tackle the volume and complexity of the content taught at the foundation level, the incorporation of online tools embedded within curricula provides a unique opportunity to engage students through active learning strategies in a blended design (Means B, Toyama Y, Murphy R, Baki M. Teach Coll Rec 115: 1-47, 2013). This article reports on the use of H5P as a platform to foster self-paced and self-directed learning, critically outlining the developmental process involved in scaffolding activities to learning outcomes. The H5P activities were embedded within the online learning management system, which enabled tracking of student access to these resources. Students were asked to complete an online survey about their perspectives on the effectiveness of H5P activities. This work is part of a comprehensive study evaluating the blended design for delivery of first-year physiology subjects.


Subject(s)
Computer-Assisted Instruction , Education, Distance , Physiology , Curriculum , Humans , Physiology/education , Problem-Based Learning , Students
2.
Eur J Neurosci ; 18(2): 373-82, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12887419

ABSTRACT

The influence of urocortin (UCN) on ingestive behaviours and brain neural activity, as measured immunohistochemically by the presence of Fos protein, was determined in mice. Rat UCN was administered by continuous intracerebroventricular (ICV) or subcutaneous (SC) infusion. ICV infusion of UCN (100 ng/h, 14 days) transiently reduced daily food and water intakes (days 1-4) but body weight was reduced from day 2 into the post-infusion period. Sodium intake was reduced from day 3 to the end of infusion. SC infusion of UCN caused similar but smaller reductions in food and water intakes and body weight, without change in sodium intake. In separate experiments, Fos immunoreactivity was increased in several brain nuclei known to be involved in the control of body fluid and energy homeostasis, e.g. central nucleus of the amygdala, median preoptic nucleus, bed nucleus of the stria terminalis and arcuate nucleus. Increased Fos expression was similar for ICV and SC infusions when measured on days 2-3 or 6-7 of infusion. In conclusion, increases of brain activity by UCN may be associated with stimulation of adrenocorticotrophic hormone release and sympathetic nervous activity, but increases may also indicate suppression of ingestive behaviours by stimulating central inhibitory mechanisms located in areas known to control body fluid and energy homeostasis.


Subject(s)
Brain/metabolism , Corticotropin-Releasing Hormone/pharmacology , Eating/drug effects , Neuroprotective Agents/pharmacology , Proto-Oncogene Proteins c-fos/metabolism , Animals , Brain/drug effects , Corticotropin-Releasing Hormone/administration & dosage , Drinking/drug effects , Immunohistochemistry , Injections, Intraventricular , Male , Mice , Mice, Inbred BALB C , Neuroprotective Agents/administration & dosage , Proto-Oncogene Proteins c-fos/drug effects , Sodium , Urocortins
3.
Methods ; 22(3): 219-25, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11071817

ABSTRACT

Phosphorothioated antisense oligodeoxynucleotides (ODNs) that were complementary to various parts of the rat or sheep mRNA encoding angiotensinogen were synthesized by conventional techniques. Their effectiveness as blockers of angiotensinogen synthesis in the brain was tested by bioassay. This involved measuring the effect of centrally administered antisense ODNs on water drinking that occurred in response to intracerebroventricular injection of hog renin. Renin-induced drinking requires brain angiotensinogen for the generation of angiotensin I and then angiotensin II to stimulate thirst. Intracerebroventricular injection of an 18-mer antisense ODN (0.5 microg twice in 24 h) complementary to the 5'-end start codon for rat angiotensinogen mRNA caused a pronounced inhibition of renin-induced drinking. This effect appeared to be specific for this region of the codon because antisense ODNs directed against other regions of rat angiotensinogen mRNA were ineffective, and renin-induced drinking was not inhibited by intracerebroventricular injection of scrambled or mismatched sequences of the effective ODN or by intraperitoneal injection of it. Intracerebroventricular injection of antisense ODN (0.5 microg twice in 24 h) did not inhibit appetite or affect water drinking in response to some other dipsogenic stimuli, thus demonstrating the specificity of its action against renin-induced drinking. By contrast, intracerebroventricular administration of 625 microg of an antisense ODN directed against the corresponding 5'-end start codon region of sheep angiotensinogen mRNA did not inhibit intracerebroventricular renin-induced drinking in sheep. These data show that while intracerebroventricularly administered antisense may be used effectively in rodents, the method is not necessarily applicable in larger mammals.


Subject(s)
Angiotensinogen/antagonists & inhibitors , Angiotensinogen/genetics , Brain/drug effects , Brain/metabolism , Oligodeoxyribonucleotides, Antisense/pharmacology , Angiotensinogen/physiology , Animals , Base Sequence , Drinking/drug effects , Drinking/physiology , Injections, Intraventricular , Male , Oligodeoxyribonucleotides, Antisense/administration & dosage , Oligodeoxyribonucleotides, Antisense/genetics , RNA, Messenger/genetics , Rats , Rats, Sprague-Dawley , Renin/administration & dosage , Sheep , Swine
4.
Endocrinology ; 140(11): 5082-6, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10537135

ABSTRACT

The purpose of the study was to determine whether iv infusion of relaxin would acutely stimulate water drinking in rats and, if it did, whether such drinking is affected by other dipsogenic stimuli or is blocked by centrally administered losartan. iv infusions of human gene 2 relaxin at doses of 25, 40, 55, or 80 microg/kg x h for 1 h induced dose-dependent water drinking in both male and female rats within 15-30 min of commencement of infusions. iv infusion of a nondipsogenic dose of angiotensin II (0.5 microg/h), combined with relaxin (40 microg/kg x h), almost tripled the relaxin-induced water intake. iv infusion of hypertonic (1 M) NaCl did not potentiate relaxin-induced drinking. Intracerebroventricular injection of the angiotensin AT1 antagonist losartan (10 microg) reduced water drinking induced by iv infusion of relaxin. The water drinking induced by iv infusion of relaxin in the rat suggests that blood-borne relaxin may be a dipsogenic hormone. Potentiation of this relaxin-induced drinking by moderate levels of circulating angiotensin II is additional evidence in support of this view. The results also indicate that a central angiotensinergic neural pathway, utilizing AT1 receptors, subserves relaxin-induced drinking.


Subject(s)
Drinking/drug effects , Relaxin/pharmacology , Angiotensin II/administration & dosage , Angiotensin II/pharmacology , Angiotensins/antagonists & inhibitors , Animals , Drug Synergism , Female , Infusions, Intravenous , Injections, Intraventricular , Kinetics , Losartan/administration & dosage , Losartan/pharmacology , Male , Rats , Rats, Sprague-Dawley , Relaxin/administration & dosage , Saline Solution, Hypertonic/pharmacology , Sodium Chloride/pharmacology
5.
Brain Res Mol Brain Res ; 50(1-2): 43-50, 1997 Oct 15.
Article in English | MEDLINE | ID: mdl-9406916

ABSTRACT

The role of centrally synthesised angiotensinogen in neural mechanisms subserving water drinking in rats was investigated by injecting antisense oligonucleotides complementary to rat angiotensinogen mRNA into the brain with the aim of inhibiting cerebral angiotensinogen synthesis. Phosphorothioate antisense oligonucleotides (18 mer) encompassing the translation start codon were injected into the lateral ventricle of rats and their responses to a number of dipsogenic stimuli tested. These were: intracerebroventricular (i.c.v.) renin, i.c.v. angiotensin II, i.c.v. carbachol, subcutaneous isoproterenol, intravenous hypertonic saline, water deprivation for 24 h or subcutaneous injection of polyethylene glycol. Antisense treatment significantly reduced (by approximately 50%) the volume of water drunk in response to i.c.v. injection of renin or subcutaneous isoproterenol, but did not reduce water intake elicited by the other dipsogenic stimuli. The i.c.v. administration of mismatch, scrambled or sense oligonucleotides did not inhibit water intake. These data suggest that centrally produced angiotensinogen may have a role in neural pathways subserving isoproterenol-induced drinking.


Subject(s)
Angiotensinogen/genetics , Drinking Behavior/drug effects , Feeding Behavior/drug effects , Oligonucleotides, Antisense/pharmacology , Animals , Injections, Intraventricular , Isoproterenol/pharmacology , Male , Neural Pathways/drug effects , Rats , Rats, Sprague-Dawley , Renin/pharmacology , Saline Solution, Hypertonic/pharmacology , Water Deprivation
6.
Clin Exp Hypertens ; 19(5-6): 993-1007, 1997.
Article in English | MEDLINE | ID: mdl-9247770

ABSTRACT

The effectiveness of antisense oligonucleotides (ODNs) to angiotensinogen on intracerebrovenricularly injected renin induced thirst was investigated. As a corollary, information would be gained about the role of centrally synthesised angiotensinogen in the neural mechanisms subserving water drinking in rats. Stable, easily synthesised phosphorothioate antisense oligonucleotides (18 mer), one of which included the sequence encompassing the translation start site, were injected into the lateral ventricle of rats. The drinking response to a number of dipsogenic stimuli was tested. Antisense significantly reduced (by about 50%) the volume of water drunk in response to intracerebroventricular (icv) renin or isoproterenol but did not reduce drinking in response to the physiological challenge of icv angiotensin II, icv carbachol, intravenous hypertonic saline, water deprivation or subcutaneous injection of polyethylene glycol. Only one out of four antisense probes gave positive results, while mismatch or scrambled oligonucleotides did not inhibit water intake. This finding reduces the probability that the results observed are non-specific. In these experiments, an ODN specific for angiotensinogen was discovered and was produced easily in large enough amounts and stabilised against intracellular nucleases without floss of cellular access or biological effect.


Subject(s)
Angiotensinogen/antagonists & inhibitors , Angiotensinogen/genetics , Drinking/drug effects , Oligonucleotides, Antisense/pharmacology , Angiotensin II/administration & dosage , Angiotensinogen/physiology , Animals , Base Sequence , Carbachol/administration & dosage , Drinking/physiology , Injections, Intraventricular , Isoproterenol/administration & dosage , Male , Oligonucleotides, Antisense/administration & dosage , Oligonucleotides, Antisense/genetics , Polyethylene Glycols/administration & dosage , RNA, Messenger/genetics , Rats , Rats, Sprague-Dawley , Renin/administration & dosage , Water Deprivation
SELECTION OF CITATIONS
SEARCH DETAIL
...