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Bioluminescence of free and poly(vinyl alcohol) cryogel-entrapped recombinant Escherichia coli cells expressing firefly luciferase.
Romanova, N A; Brovko, L Y; Makhlis, T A; Sinodova, E M; Ugarova, N N.
Biochemistry (Mosc) ; 63(5): 579-83, 1998 May.
Article in English | MEDLINE | ID: mdl-9632896

ABSTRACT

Bioluminescence of free and poly(vinyl alcohol) cryogel-entrapped recombinant E. coli cells expressing firefly luciferase was investigated. It was shown that bioluminescence intensity and time-course of the bioluminescent signal changed upon immobilization and depended on intracellular ATP concentration and permeability of the cell membrane.


Subject(s)
Escherichia coli/enzymology , Escherichia coli/genetics , Luciferases/genetics , Luciferases/metabolism , Adenosine Triphosphate/metabolism , Animals , Cell Membrane Permeability , Coleoptera/enzymology , Coleoptera/genetics , Genes, Insect , Kinetics , Luminescent Measurements , Polyvinyl Alcohol , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Recombination, Genetic
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