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1.
J Appl Microbiol ; 125(4): 1208-1215, 2018 Oct.
Article in English | MEDLINE | ID: mdl-29920871

ABSTRACT

AIMS: To improve RT-qPCR with an internal control and a synthetic standard curve to detect HEV in HIV co-infected patients. METHODS AND RESULTS: A single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA) synthetic curve were designed, compared to the international reference panel for HEV genotypes, and tested to quantify and detect a reference panel for HEV genotypes. The detection limit of the RNA synthetic curve (50 copies per ml) was better than the DNA synthetic curve (100 copies per ml) and the WHO standard curve (250 copies per ml). Then, 280 serum samples from HIV-positive patients were tested for HEV RNA, which was detected in 3·6% of serum samples. The viral load ranged from 2 × 102 copies per ml to 4·78 × 108 copies per ml. HEV IgM/IgG antibodies were not detected in the RNA-positive patients. Sequencing analysis of HEV showed that the virus belongs to genotype 3 (HEV GT3). CONCLUSIONS: Real-time PCR was a useful tool to estimate co-infection with HEV/HIV, even in patients with low viral loads and undetectable anti-HEV IgG and IgM antibodies. SIGNIFICANCE AND IMPACT OF THE STUDY: Hepatitis E virus genotype 3 (HEV GT3) has been associated with silent chronic hepatitis and cirrhosis in HIV-positive subjects worldwide, but there is a lack of data on this co-infection in Brazil.


Subject(s)
HIV Infections/blood , Hepatitis Antibodies/blood , Hepatitis E virus/isolation & purification , Hepatitis E/virology , Immunoglobulin G/blood , Immunoglobulin M/blood , Adult , Brazil , Coinfection/blood , Coinfection/virology , Female , Genotype , HIV Infections/virology , Hepatitis E/blood , Hepatitis E virus/genetics , Hepatitis E virus/immunology , Hepatitis E virus/physiology , Humans , Male , Middle Aged , Real-Time Polymerase Chain Reaction , Viral Load
2.
Int J STD AIDS ; 19(5): 321-6, 2008 May.
Article in English | MEDLINE | ID: mdl-18482962

ABSTRACT

Shifting of hepatitis A virus (HAV) epidemiology from a high towards an intermediate endemicity pattern and use of antiretroviral therapy increased the risk of HIV/HAV coinfection in developing countries. The aim of this study was to investigate the presence of HAV markers in a cohort of HIV-infected patients from 1988 to 2004. The presence of serum anti-HAV antibodies and HAV-RNA by real-time polymerase chain reaction was investigated in 581 patients. Total anti-HAV antibodies was found in 464/581 (79.8%) patients, however, a changing epidemiologic pattern of hepatitis A among HIV-infected patients from 1988 to 2004 was observed. Among patients susceptible to HAV (n = 117), 5 (4.2%) were coinfected with HAV, all of them had IgM anti-HAV antibodies and were serum HAV-RNA-positive. The high prevalence of anti-HAV antibodies in HIV-infected patients suggests that screening tests for anti-HAV antibodies should be performed before implementation of hepatitis A vaccination, especially in those patients from endemic countries.


Subject(s)
HIV Infections/complications , Hepatitis A Vaccines/administration & dosage , Hepatitis A virus/immunology , Adolescent , Adult , Brazil/epidemiology , Child , Child, Preschool , Cohort Studies , Female , HIV Infections/virology , Hepatitis A Antibodies/blood , Hepatitis A Vaccines/therapeutic use , Hepatitis A virus/isolation & purification , Humans , Infant , Male , Middle Aged
3.
Virus Genes ; 23(2): 193-202, 2001.
Article in English | MEDLINE | ID: mdl-11724274

ABSTRACT

We have investigated the phenotypic and genotypic susceptibility of 14 HIV-1 strains isolated from individuals failing HAART therapy to protease inhibitors (PI). Proviral and plasma viral pol gene fragment were amplified, sequenced and subtyped. Nine samples clustered with protease subtype B reference strains and the remaining samples were classified as non-B subtype corresponding to subtype F (n = 4) and subtype A (n = 1). Although all patients were treated with similar P1 drug regimen, the non-B subtype isolates did not present the L90M and 184V mutations and used mainly G48V and V82A/F to achieve drug resistance. A strong cross-resistance phenotype among all four PI was associated with the mutation L90M in the subtype-B isolates, and with G48V and V82A/F in the non-B counterparts. This observation revealed that the non-B viruses tested had specific genotypic characteristics contrasting with the subtype-B isolates.


Subject(s)
Antiretroviral Therapy, Highly Active , Drug Resistance, Microbial/genetics , HIV Infections/drug therapy , HIV-1/genetics , Mutation , Amino Acid Sequence , Genotype , HIV Infections/virology , HIV-1/drug effects , Humans , Molecular Sequence Data , Phenotype , Sequence Homology, Amino Acid
4.
Virology ; 275(1): 107-15, 2000 Sep 15.
Article in English | MEDLINE | ID: mdl-11017792

ABSTRACT

Development of drug resistance is the inevitable consequence of incomplete suppression of virus plasma levels in HIV-1-infected patients treated with highly active antiretroviral therapy. Resistance mutations previously characterized have been found in B subtype viruses of developed countries. Moreover, mutation profiles for non-B and more divergent B subtype viruses found in developing countries shall be analyzed together with their ex vivo phenotyping in order to establish an exact correlation between the genotyping data and the clinical management counseling for those uncommon virus subtypes. In the present study, we evaluated the mutation profile for individuals infected with B subtype and non-B subtype viruses. Viral DNA fragments corresponding to the RT gene were amplified, sequenced, and subtyped. Phenotyping analysis for reverse transcriptase nucleoside (NRTI) and nonnucleoside inhibitor susceptibility was performed using the recombinant virus assay technology. Brazilian non-B subtypes (subtype F, n = 4, and subtype A, n = 1) isolates showed essentially the same B subtype mutation profile, presenting an NRTI drug resistance with similar MIC50% and MIC90% values for all drugs analyzed regardless of their subtypes. A strong cross-resistance phenotype among AZT, 3TC, and abacavir could be seen in all isolates analyzed. A novel result was that some RT sequences not only revealed the presence of G333D/E mutations but also correlated to the presence of mutation T386I that could abrogate the M184V-surpassing effect of L210W or L210W plus G333D/E. These findings suggest that Brazilian non-B subtype HIV-1 strains use an identical RT drug resistance mutation pattern when compared to B isolates and will contribute to the validation of the genotypic and phenotypic tests in these predominant worldwide-spread viral variants.


Subject(s)
Acquired Immunodeficiency Syndrome/drug therapy , Anti-HIV Agents/therapeutic use , Drug Resistance, Multiple/genetics , HIV Reverse Transcriptase/genetics , HIV-1/classification , HIV-1/enzymology , Reverse Transcriptase Inhibitors/therapeutic use , Acquired Immunodeficiency Syndrome/epidemiology , Acquired Immunodeficiency Syndrome/virology , Amino Acid Sequence , Amino Acid Substitution/genetics , Anti-HIV Agents/administration & dosage , Anti-HIV Agents/pharmacology , Brazil/epidemiology , DNA Mutational Analysis , Drug Resistance, Microbial , Drug Therapy, Combination , Female , Genetic Variation/genetics , Genotype , HIV Reverse Transcriptase/antagonists & inhibitors , HIV Reverse Transcriptase/metabolism , HIV-1/drug effects , HIV-1/genetics , Humans , Male , Molecular Sequence Data , Mutation/genetics , Phenotype , Phylogeny , Reverse Transcriptase Inhibitors/administration & dosage , Reverse Transcriptase Inhibitors/pharmacology , Risk Factors , Sequence Alignment , Time Factors , Treatment Failure
5.
J Acquir Immune Defic Syndr (1988) ; 5(12): 1212-23, 1992 Dec.
Article in English | MEDLINE | ID: mdl-1453332

ABSTRACT

A clinical AIDS case definition is needed for surveillance in countries where the CDC case definition is not practical. To derive such a definition, we compared 110 HIV-seropositive and 135 randomly selected HIV-seronegative adult medical-ward inpatients in Brazil. Multivariate analysis of clinical signs and symptoms and simple diagnoses resulted in a discriminant function with sensitivity of 89% and specificity of 96% in predicting for AIDS. These data were the empirical basis for a clinical definition of AIDS in adults drafted in a Caracas, Venezuela, workshop sponsored by the Pan American Health Organization. The revised "Caracas" definition presented here requires a positive HIV serology, the absence of cancer or other cause of immunosuppression, plus > or = 10 cumulative points, as follows: Kaposi's sarcoma (10 points); extrapulmonary/noncavitary pulmonary tuberculosis (10); oral candidiasis or hairy leukoplakia (5); cavitary pulmonary/unspecified tuberculosis (5); herpes zoster < 60 years of age (5); CNS dysfunction (5); diarrhea > or = 1 month (2); fever > or = 1 month (2); cachexia or > 10% weight loss (2); asthenia > or = 1 month (2); persistent dermatitis (2); anemia, lymphopenia, or thrombocytopenia (2); persistent cough or any pneumonia except TB (2); and lymphadenopathy > or = 1 cm at > or = 2 noninguinal sites for > or = 1 month (2). This definition has a sensitivity of 95% and a specificity of 100% (91% without HIV serology) when applied to the Brazilian patients in this study. The Caracas definition has been adopted by Brazil, Honduras, and Surinam, and is in validation elsewhere. The use of a reasonably sensitive and specific case definition commensurate with available diagnostic resources should facilitate AIDS surveillance in developing countries.


Subject(s)
Acquired Immunodeficiency Syndrome/diagnosis , HIV-1 , HIV-2 , Acquired Immunodeficiency Syndrome/complications , Acquired Immunodeficiency Syndrome/epidemiology , Adolescent , Adult , Analysis of Variance , Brazil/epidemiology , Humans , Middle Aged , Multivariate Analysis , Population Surveillance , Tuberculosis, Pulmonary/complications , World Health Organization
6.
AIDS ; 5(11): 1293-9, 1991 Nov.
Article in English | MEDLINE | ID: mdl-1768377

ABSTRACT

Analysis of sera from hospitalized Brazilian patients by whole-virus lysate-based enzyme immunoassay and Western blot indicated that 0.4% were reactive to HIV-2 alone while 4% were reactive to both HIV-1 and HIV-2. When these sera were tested for HIV antibody by type-specific peptide enzyme immunoassays, dual seropositivity was confirmed in only 0.4% of patients. To define genetically the HIV strains within the population, we analyzed peripheral blood mononuclear cells from selected seropositive patients for the presence of HIV-1 and HIV-2 proviral DNA using the polymerase chain reaction (PCR). Independent primers/probes sets were used for the amplification and detection of viral sequences from the long terminal repeat (LTR), gag, and protease (prt) gene regions. Our findings confirmed the serologic evidence of HIV-2 in Brazil and determined the extent of mixed HIV-1 and HIV-2 infections. Detailed evaluation of the amplified viral protease sequences by endonuclease restriction analysis and DNA sequencing independently confirmed mixed HIV-1 and HIV-2 infections in the two patients seropositive for HIV-1 and HIV-2. The data further indicated that these isolates are distinct from the HIV laboratory standards. We interpret the combination of culture and PCR findings to demonstrate the presence of both HIV-1 and HIV-2 in Brazil.


Subject(s)
HIV Infections/microbiology , HIV-1/isolation & purification , HIV-2/isolation & purification , Base Sequence , Brazil , DNA Probes , DNA, Viral/genetics , Genes, gag , HIV Protease/genetics , HIV-1/genetics , HIV-2/genetics , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Repetitive Sequences, Nucleic Acid
7.
Mem. Inst. Oswaldo Cruz ; 83(3): 305-11, jul.-set. 1988. ilus, tab
Article in English | LILACS | ID: lil-76157

ABSTRACT

Alteraçöes imunológicas na Síndrome de Imunodeficiência Adquirida em pacientes do Rio de Janeiro, Brasil. O perfil imunológico de 15 pacientes com Síndrome de Imunodeficiência Adquirida (AIDS) e 11 com Síndrome de Linfadenipatia Crônica, foram estudados. Os pacientes com AIDS mostraram reduzida percentagem de linfócitos T (CD3) totais e T auxiliares (CD4), aumento relativo no número de linfócito T-supressores (CD8) e uma marcante inversäo na relaçäo T-auxiliares/supressores (CD4/CD8). A resposta linfoproliferativa para PHA, ConA, PPD e PWN, estava diminuída. Foi também observado hipergamaglobulinemia e níveis aumentados de complexos imunes circulantes. Os pacientes com Síndrome de Linfadenopatia Crônica também mostraram importantes alteraçöes imunológicas, mas näo täo intensas como nos de AIDS. Estes dados säo similares aos observados nos Estados Unidos e na Europa (U)


Subject(s)
Adolescent , Adult , Middle Aged , Humans , Male , Female , AIDS-Related Complex/immunology , Acquired Immunodeficiency Syndrome/immunology , Brazil , Immunoglobulins/analysis , T-Lymphocytes, Helper-Inducer/analysis
8.
Mem Inst Oswaldo Cruz ; 83(3): 305-11, 1988.
Article in English | MEDLINE | ID: mdl-2978758

ABSTRACT

The immunological profile of acquired immunodeficiency syndrome (AIDS) and chronic lymphadenopathy syndrome (CLAS) in 15 and 11 Brazilian patients, respectively, was studied. The AIDS patients showed reduced percentage of total T (CD3) and T-helper-inducer (CD4) lymphocytes, relative increase in numbers of T-suppressor-cytotoxic (CD8) cells and a marked inversion of T-helper-inducer/suppressor-cytotoxic (CD4/CD8) ratio. Lymphoproliferative responses to PHA, ConA, PPD and PWM were diminished. Hypergammaglobulinemia and high levels of circulating immune complexes were also found. The CLAS patients also showed important immunological alterations, but not so intense as those with AIDS. These data seems to be similar to those observed in other parts of the world.


Subject(s)
AIDS-Related Complex/immunology , Acquired Immunodeficiency Syndrome/immunology , Adolescent , Adult , Antigen-Antibody Complex/analysis , Brazil , Female , Homosexuality , Humans , Immunoglobulins/analysis , Male , Middle Aged , Sexual Behavior , T-Lymphocytes/analysis , T-Lymphocytes, Helper-Inducer/analysis
9.
Mem Inst Oswaldo Cruz ; 82(4): 453-6, 1987.
Article in English | MEDLINE | ID: mdl-3507914

ABSTRACT

A retrovirus infecting a Brazilian AIDS patient was isolated and characterized in terms of its reactivity with sera from individuals infected with human immunodeficiency viruses 1 and 2 (HIV-1 and HIV-2). The Western blot analysis revealed that the Brazilian isolate is very similar to the well characterized HIV-1 strain. The serum of the patient from whom the virus was isolated did not react with the 140 kDa envelope glycoprotein specific for HIV-2.


Subject(s)
HIV Antigens/analysis , HIV-1/isolation & purification , Adult , Blotting, Western , Brazil , Enzyme-Linked Immunosorbent Assay , HIV Antibodies/immunology , HIV-1/immunology , HIV-2/immunology , Humans , Male
11.
Braz J Med Biol Res ; 18(4): 519-26, 1985.
Article in English | MEDLINE | ID: mdl-3836708

ABSTRACT

We describe a sensitive radioassay for detecting erythrocyte-associated immunoglobulin employing the immunoglobulin-specific reagent Staphylococcus aureus protein A. The assay was used to determine the extent to which erythrocytes from patients with different connective tissue disease bind radiolabelled protein A. Increased amounts of protein A were bound by erythrocytes from patients with systemic lupus erythematosus or with progressive systemic sclerosis when compared to a control group, whereas there was no significant binding to erythrocytes from patients with rheumatoid arthritis or with polymyositis-dermatomyositis. The assay, because of its high sensitivity and of the availability of the reagent in pure form, should be useful for the investigation of cell-bound antibodies and for the differential diagnosis of connective tissue diseases.


Subject(s)
Connective Tissue Diseases/diagnosis , Erythrocytes/immunology , Immunoglobulin G/metabolism , Staphylococcal Protein A , Diagnosis, Differential , Erythrocytes/metabolism , Humans , Protein Binding , Radioimmunoassay
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