Subject(s)
Endothelial Cells/pathology , Hematopoietic Stem Cell Transplantation/adverse effects , Hepatic Veno-Occlusive Disease/diagnosis , Liver/pathology , Portal Vein/pathology , Adolescent , Adult , Antigens, CD/immunology , Antineoplastic Agents/therapeutic use , Cell Count , Endothelial Cells/immunology , Female , Flow Cytometry , Hematologic Neoplasms/immunology , Hematologic Neoplasms/pathology , Hematologic Neoplasms/therapy , Hepatic Veno-Occlusive Disease/etiology , Hepatic Veno-Occlusive Disease/immunology , Hepatic Veno-Occlusive Disease/pathology , Humans , Immunophenotyping , Liver/immunology , Male , Middle Aged , Portal Vein/immunology , Transplantation, HomologousABSTRACT
The flow cytometry becomes a more and more largely applied technique. However, the sufficient novelty of technique has no worked-out standards of diagnostic of many diseases. The lacking of external control of quality promotes development of large variety of approaches to diagnostic of diseases and impossibility to compare the study results from different laboratories. The paroxysmal night hemoglobinuria is an acquired clonal disease characterized by proliferation of stem cells with partial or total loss of expression of glykosylphosphosphatidyl inositol anchor needed to conjugate a number of surface proteins. The flow cytometry is a basic technique of detection and monitoring of clone of paroxysmal night hemoglobinuria. The article presents the results of paroxysmal night hemoglobinuria testing of 8 patients in 6 independent laboratories using flow cytometry by standard protocol recommended by the International society of clinical cytometrists (ICCS).