ABSTRACT
The stability of native human leukocyte IFN-alpha was investigated after one year storage at different temperatures, or after sterilization with 60Co-irradiation by determining the total antiviral activity of samples. Inactivation of VSV and Aujeszky virions by 60Co-irradiation was directly related to the radiolysis of samples, indicating the uselessness of this procedure for sterilization of IFN-alpha preparations. The presence and proportion of subtypes in the stored or irradiated preparations (with 50 and 25-70% inactivation, respectively) was analysed by chromatofocusing, comparing their patterns with that of the untreated controls. A logarithmic correlation was found between the pI values and temperature/irradiation sensitivity of subtypes.
Subject(s)
Antiviral Agents/radiation effects , Interferon Type I/radiation effects , Sterilization/methods , Antiviral Agents/pharmacology , Cobalt Radioisotopes , Drug Stability , Drug Storage , Gamma Rays , Interferon Type I/classification , Interferon Type I/pharmacology , Isoelectric Point , Temperature , Viruses/radiation effectsABSTRACT
The biological half-life of synthetic, radiochemically pure, biologically active [3H]8-arginine-vasopressin ([3H]AVP), the distribution of radioactivity among the organs and the in-vivo metabolism of the hormone were studied in the rat. The half-life calculated from the [3H]AVP radioactivities isolated from the blood was found to be 1.74 +/- 0.22 (S.D.) min in the fast phase, and 16.98 +/- 1.01 min in the slow phase. The half-lives of total radioactivity were longer in both phases. The radioactivity accumulated to the greatest extents in the adenohypophysis and small intestine. The radioactive substance was accumulated more by the kidney than by the liver, but the hormone underwent inactivation more quickly in the liver.
Subject(s)
Arginine Vasopressin/metabolism , Animals , Arginine Vasopressin/blood , Chromatography, High Pressure Liquid , Half-Life , Intestine, Small/metabolism , Kidney/metabolism , Liver/metabolism , Male , Pituitary Gland, Anterior/metabolism , Rats , Rats, Inbred StrainsABSTRACT
Human erythrocytes were treated with highly tritiated [3H]iodoacetate under conditions when half of the label became attached to glyceraldehyde-3-phosphate dehydrogenase. After fixation, the cells were subjected to electron microscopic autoradiography. For the evaluation of distribution of grains, which were relatively few, a computerized method was developed. Statistical analysis of data showed the significant adherence of grains to the cell membrane. The results support the view that glyceraldehyde-3-phosphate dehydrogenase is localized near the membrane in the intact erythrocyte.
