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1.
medRxiv ; 2021 Feb 27.
Article in English | MEDLINE | ID: mdl-33655273

ABSTRACT

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) causes coronavirus disease-19 (COVID-19), a respiratory illness that can result in hospitalization or death. We investigated associations between rare genetic variants and seven COVID-19 outcomes in 543,213 individuals, including 8,248 with COVID-19. After accounting for multiple testing, we did not identify any clear associations with rare variants either exome-wide or when specifically focusing on (i) 14 interferon pathway genes in which rare deleterious variants have been reported in severe COVID-19 patients; (ii) 167 genes located in COVID-19 GWAS risk loci; or (iii) 32 additional genes of immunologic relevance and/or therapeutic potential. Our analyses indicate there are no significant associations with rare protein-coding variants with detectable effect sizes at our current sample sizes. Analyses will be updated as additional data become available, with results publicly browsable at https://rgc-covid19.regeneron.com.

2.
medRxiv ; 2021 Jun 10.
Article in English | MEDLINE | ID: mdl-33619501

ABSTRACT

SARS-CoV-2 enters host cells by binding angiotensin-converting enzyme 2 (ACE2). Through a genome-wide association study, we show that a rare variant (MAF = 0.3%, odds ratio 0.60, P=4.5×10-13) that down-regulates ACE2 expression reduces risk of COVID-19 disease, providing human genetics support for the hypothesis that ACE2 levels influence COVID-19 risk. Further, we show that common genetic variants define a risk score that predicts severe disease among COVID-19 cases.

3.
Genes Immun ; 15(6): 370-7, 2014 Sep.
Article in English | MEDLINE | ID: mdl-24898387

ABSTRACT

We analyzed two West African samples (Guinea-Bissau: n=289 cases and 322 controls; The Gambia: n=240 cases and 248 controls) to evaluate single-nucleotide polymorphisms (SNPs) in Epiregulin (EREG) and V-ATPase (T-cell immune regulator 1 (TCIRG1)) using single and multilocus analyses to determine whether previously described associations with pulmonary tuberculosis (PTB) in Vietnamese and Italians would replicate in African populations. We did not detect any significant single locus or haplotype associations in either sample. We also performed exploratory pairwise interaction analyses using Visualization of Statistical Epistasis Networks (ViSEN), a novel method to detect only interactions among multiple variables, to elucidate possible interaction effects between SNPs and demographic factors. Although we found no strong evidence of marginal effects, there were several significant pairwise interactions that were identified in either the Guinea-Bissau or the Gambian samples, two of which replicated across populations. Our results indicate that the effects of EREG and TCIRG1 variants on PTB susceptibility, to the extent that they exist, are dependent on gene-gene interactions in West African populations as detected with ViSEN. In addition, epistatic effects are likely to be influenced by inter- and intra-population differences in genetic or environmental context and/or the mycobacterial lineages causing disease.


Subject(s)
Epiregulin/genetics , Genetic Predisposition to Disease/genetics , Polymorphism, Single Nucleotide , Tuberculosis, Pulmonary/genetics , Vacuolar Proton-Translocating ATPases/genetics , Adult , Alleles , Black People/genetics , Epistasis, Genetic , Gambia , Gene Frequency , Genetic Predisposition to Disease/ethnology , Genotype , Guinea-Bissau , Humans , Linkage Disequilibrium , Logistic Models , Male , Odds Ratio , Tuberculosis, Pulmonary/ethnology
4.
Public Health Genomics ; 13(5): 284-91, 2010.
Article in English | MEDLINE | ID: mdl-19815999

ABSTRACT

Pharmacogenomics (PGx) and pharmacogenetics (PGt) are emerging interdisciplinary areas recently defined by the regulatory authorities at an international level as 'the investigation of variations of DNA and RNA characteristics as related to drug response' (PGx), and the study of 'the influence of variations in DNA sequence on drug efficacy and toxicity' (PGt). In recent years a number of studies have in fact produced growing evidence that, besides the effects of age, sex, diseases, and different drugs interactions, genetic factors play a role in the inter-individual variability of drugs response. The increasing genomic knowledge has also raised the profile and role of the so called 'genomic biomarkers' (GBs) in drug development, approval, and clinical use. The aims of this review are to (a) revisit the general understanding of the role of genomics and genetics in drug response, (b) provide an update on the definition and classification criteria of GBs as recently defined at a global level by regulatory agencies such as the European Medicines Agency and the Food and Drug Administration, and (c) illustrate with some examples current and potential applications of biomarkers in clinical practice and in drug development.


Subject(s)
Drug Approval , Pharmacogenetics , Humans , Polymorphism, Single Nucleotide
5.
Genes Immun ; 8(6): 456-67, 2007 Sep.
Article in English | MEDLINE | ID: mdl-17611589

ABSTRACT

We investigated the role of DC-SIGN (CD209), long pentraxin 3 (PTX3) and vitamin D receptor (VDR) gene single nucleotide polymorphisms (SNPs) in susceptibility to pulmonary tuberculosis (TB) in 321 TB cases and 347 healthy controls from Guinea-Bissau. Five additional, functionally relevant SNPs within toll-like receptors (TLRs) 2, 4 and 9 were typed but found, when polymorphic, not to affect host vulnerability to pulmonary TB. We did not replicate an association between SNPs in the DC-SIGN promoter and TB. However, we found that two polymorphisms, one in DC-SIGN and one in VDR, were associated in a nonadditive model with disease risk when analyzed in combination with ethnicity (P=0.03 for DC-SIGN and P=0.003 for VDR). In addition, PTX3 haplotype frequencies significantly differed in cases compared to controls and a protective effect was found in association with a specific haplotype (OR 0.78, 95% CI 0.63-0.98). Our findings support previous data showing that VDR SNPs modulate the risk for TB in West Africans and suggest that variation within DC-SIGN and PTX3 also affect the disease outcome.


Subject(s)
C-Reactive Protein/genetics , Cell Adhesion Molecules/genetics , Lectins, C-Type/genetics , Polymorphism, Single Nucleotide , Receptors, Calcitriol/genetics , Receptors, Cell Surface/genetics , Serum Amyloid P-Component/genetics , Adolescent , Adult , C-Reactive Protein/metabolism , Case-Control Studies , Cell Adhesion Molecules/metabolism , Female , Genetic Predisposition to Disease , Genotype , Guinea-Bissau , Haplotypes , Humans , Lectins, C-Type/metabolism , Male , Mycobacterium tuberculosis , Receptors, Calcitriol/metabolism , Receptors, Cell Surface/metabolism , Serum Amyloid P-Component/metabolism , Toll-Like Receptors/genetics , Toll-Like Receptors/metabolism , Tuberculosis, Pulmonary/genetics , Tuberculosis, Pulmonary/metabolism
6.
Int J Immunogenet ; 33(1): 11-5, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16426236

ABSTRACT

We identified a novel trinucleotide (ATA)n repeat polymorphism in intron 8 of SLC11AI, a candidate gene for susceptibility to tuberculosis (TB) infection. We characterized the frequency of this polymorphism in 485 individuals originating from eight globally diverse human populations and compared the distribution of (ATA)n alleles in 146 adults and in 80 cord blood samples from newborns in the Gambian population. Lastly, we tested for association of this microsatellite with pulmonary TB in 318 TB cases and 146 controls in the Gambian population. We found no significant difference in frequency or distribution of alleles in adult and cord blood samples, and we found no significant association between this marker and pulmonary TB.


Subject(s)
Cation Transport Proteins/genetics , Genetic Predisposition to Disease , Trinucleotide Repeats , Tuberculosis, Pulmonary/genetics , Case-Control Studies , Gene Frequency , Humans , Polymorphism, Genetic
7.
Tuberculosis (Edinb) ; 84(6): 347-52, 2004.
Article in English | MEDLINE | ID: mdl-15525557

ABSTRACT

SETTING: Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide. Studies in a murine model of pulmonary TB have identified a role for Toll-like receptor 4 (TLR4) in the development of chronic lung infection with Mycobacterium tuberculosis. The Asp299Gly polymorphism in the human TLR4 gene is associated with in vivo hyporesponsiveness to lipopolysaccharide (LPS) in Caucasians. OBJECTIVE: To determine whether TLR4 Asp299Gly influences LPS responses or susceptibility to pulmonary TB in humans in a Gambian population sample. DESIGN: We compared whole blood monokine responses to LPS in 245 healthy blood donors stratified by TLR4 Asp299Gly genotype to assess whether this polymorphism was functional in this population. A case-control study of 640 subjects was conducted to investigate whether TLR4 Asp299Gly was associated with TB. RESULTS: LPS-induced tumour necrosis factor, interleukin-1 beta and interleukin-10 production was not influenced by TLR4 Asp299Gly genotype. There was no association between TLR4 Asp299Gly and TB. CONCLUSION: Our data suggest that TLR4 Asp299Gly has no influence on monocyte LPS responses or susceptibility to TB in Gambians and could be an ancient neutral polymorphism.


Subject(s)
Lipopolysaccharides/immunology , Membrane Glycoproteins/genetics , Receptors, Cell Surface/genetics , Tuberculosis, Pulmonary/genetics , Adolescent , Adult , Case-Control Studies , Gambia , Gene Frequency , Genetic Predisposition to Disease/genetics , Genotype , Humans , Interleukin-1/analysis , Interleukin-10/analysis , Male , Membrane Glycoproteins/immunology , Middle Aged , Polymorphism, Genetic/genetics , Receptors, Cell Surface/immunology , Toll-Like Receptor 4 , Toll-Like Receptors , Tuberculosis, Pulmonary/ethnology , Tuberculosis, Pulmonary/immunology , Tumor Necrosis Factor-alpha/analysis , Up-Regulation
8.
Tissue Antigens ; 62(1): 72-5, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12859597

ABSTRACT

Caucasians carry TNFA-308*2 in the 8.1 ancestral haplotype (AH) (HLA-A1,B8,DR3). In Gambians, TNFA-308*2 occurs without HLA-B8 or -DR3, suggesting an independent effect of TNFA-308 on disease. Hence we sought a segment of the 8.1 AH in Gambians. BAT1 (intron 10)*2 was selected as a specific marker of the haplotype and was found with TNFA-308*2 in Gambians. Samples homozygous at TNFA-308 and BAT1 (intron 10) demonstrated identity between the African TNFA-308*2 haplotype, the 8.1AH and the Asian diabetogenic 58.1AH (HLA-A33,B58,DR3) across a region spanning BAT1, ATP6G, IKBL, LTA, TNFA, LTB, LST-1 and AIF-1. Conservation of this block in geographically distinct populations suggests a common evolutionary origin and challenges current views of the role of TNFA-308*2 in disease.


Subject(s)
HLA-A1 Antigen/genetics , HLA-B8 Antigen/genetics , HLA-DR3 Antigen/genetics , Haplotypes , Tumor Necrosis Factor-alpha/genetics , Asian People/genetics , Biomarkers , China , Gambia , HLA-A1 Antigen/immunology , HLA-B8 Antigen/immunology , HLA-DR3 Antigen/immunology , Homozygote , Humans , Introns , Microsatellite Repeats , Polymorphism, Single Nucleotide , White People/genetics
9.
Am J Hum Genet ; 67(4): 901-25, 2000 Oct.
Article in English | MEDLINE | ID: mdl-10986042

ABSTRACT

Two dinucleotide short tandem-repeat polymorphisms (STRPs) and a polymorphic Alu element spanning a 22-kb region of the PLAT locus on chromosome 8p12-q11.2 were typed in 1,287-1,420 individuals originating from 30 geographically diverse human populations, as well as in 29 great apes. These data were analyzed as haplotypes consisting of each of the dinucleotide repeats and the flanking Alu insertion/deletion polymorphism. The global pattern of STRP/Alu haplotype variation and linkage disequilibrium (LD) is informative for the reconstruction of human evolutionary history. Sub-Saharan African populations have high levels of haplotype diversity within and between populations, relative to non-Africans, and have highly divergent patterns of LD. Non-African populations have both a subset of the haplotype diversity present in Africa and a distinct pattern of LD. The pattern of haplotype variation and LD observed at the PLAT locus suggests a recent common ancestry of non-African populations, from a small population originating in eastern Africa. These data indicate that, throughout much of modern human history, sub-Saharan Africa has maintained both a large effective population size and a high level of population substructure. Additionally, Papua New Guinean and Micronesian populations have rare haplotypes observed otherwise only in African populations, suggesting ancient gene flow from Africa into Papua New Guinea, as well as gene flow between Melanesian and Micronesian populations.


Subject(s)
Alu Elements/genetics , Chromosomes, Human, Pair 8/genetics , Genetic Variation/genetics , Haplotypes/genetics , Phylogeny , Polymorphism, Genetic/genetics , Tandem Repeat Sequences/genetics , Africa South of the Sahara/ethnology , Alleles , Animals , Dinucleotide Repeats/genetics , Evolution, Molecular , Gene Frequency , Hominidae/genetics , Humans , Linkage Disequilibrium , Micronesia , Papua New Guinea , Sequence Deletion/genetics
10.
Genomics ; 65(2): 104-12, 2000 Apr 15.
Article in English | MEDLINE | ID: mdl-10783257

ABSTRACT

We showed recently that the yeast mitochondrial intermediate peptidase (YMIP polypeptide; gene symbol, OCT1) promotes mitochondrial iron uptake by catalyzing the maturation of iron-utilizing proteins and exacerbates the mitochondrial iron accumulation that results from loss of yeast frataxin, a mitochondrial protein required for mitochondrial iron efflux. This suggests that the human MIP (HMIP polypeptide; gene symbol MIPEP) may be one of the loci predicted to influence the clinical manifestations of Friedreich's ataxia (FRDA), an autosomal recessive neurodegenerative disease caused by lack of human frataxin. To begin to test this hypothesis, we have characterized HMIP at the functional and genomic levels. We show that HMIP can complement a yeast knock-out mutant lacking YMIP, demonstrating that HMIP and YMIP are functional homologues. The MIPEP gene spans 57 kb and consists of 19 exons that correlate with the functional domains of HMIP. Primer extension analysis has identified a major transcript of the MIPEP gene expressed differentially and predominantly in tissues with high oxygen consumption, while sequence analysis of approximately 2 kb of 5'-flanking DNA has revealed putative Mt1/3/4, NF-kappaB, and AP-1 elements that may regulate MIPEP expression in these tissues. Using a new polymorphic (CA)(n) repeat in intron 4, MIPEP has been genetically mapped within a 7-cM interval between markers D13S283 and D13S217 on 13q12. This work provides the basis for molecular analysis of MIPEP in FRDA and possibly other neurodegenerative diseases.


Subject(s)
Iron-Binding Proteins , Metalloendopeptidases/genetics , Phosphotransferases (Alcohol Group Acceptor)/genetics , Base Sequence , Cell Division , Chromosomes, Human, Pair 13 , Contig Mapping , DNA, Fungal , Family Health , Fungal Proteins/biosynthesis , Fungal Proteins/genetics , Genetic Complementation Test , Genetic Linkage , Humans , Metalloendopeptidases/biosynthesis , Molecular Sequence Data , Promoter Regions, Genetic , Repetitive Sequences, Nucleic Acid , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Transcription, Genetic , Yeasts/genetics , Frataxin
11.
Hum Genet ; 104(6): 480-5, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10453736

ABSTRACT

Familial amyloidotic polyneuropathy (FAP) is a lethal autosomal dominant type of amyloidosis resulting from the deposition of transthyretin (ATTR) variants in the peripheral and autonomic nervous systems. ATTR V30M-associated FAP exhibits marked genetic anticipation in some families, with clinical symptoms developing at an earlier age in successive generations. The genetic basis of this phenomenon in FAP is unknown. Anticipation has been associated with the dynamic expansion of trinucleotide repeats in several neurodegenerative disorders, such as Huntington disease, myotonic dystrophy, and fragile X syndrome. We have used the repeat expansion detection (RED) assay to screen affected members of Portuguese FAP kindreds for expansion of any of the ten possible trinucleotide repeats. Nine generational pairs with differences in their age of onset greater than 12 years and a control pair with identical ages of onset were tested. No major differences were found in the lengths of the ten trinucleotide repeats analyzed. The distribution of the maximal repeat sizes was consistent with reported studies in unrelated individuals with no known genetic disease. The present data do not support a role for trinucleotide repeat expansions as the molecular mechanism underlying anticipation in Portuguese FAP.


Subject(s)
Amyloid Neuropathies/genetics , Anticipation, Genetic , Trinucleotide Repeat Expansion , Adolescent , Adult , Age of Onset , Alleles , Child , Female , Genotype , Humans , Male , Pedigree , Polymerase Chain Reaction/methods , Portugal
15.
J Biol Chem ; 273(18): 11177-82, 1998 May 01.
Article in English | MEDLINE | ID: mdl-9556606

ABSTRACT

Survivin is a new IAP apoptosis inhibitor expressed during development and in human cancer in vivo. The coding strand of the survivin gene was extensively complementary to that of effector cell protease receptor-1 (EPR-1), prompting the present investigation on the origin and functional relationship of these two transcripts. Southern blots of genomic DNA were consistent with the presence of multiple, evolutionarily conserved, EPR-1/Survivin-related genes. By pulsed field gel electrophoresis and single- and two-color fluorescence in situ hybridization, these were contained within a contiguous physical interval of 75-130 kilobases (kb) on chromosome 17q25. In Northern blots, a single strand-specific probe identified a 1.3-kb EPR-1 mRNA broadly distributed in normal adult and fetal tissues, structurally distinct from the 1.9-kb Survivin transcript expressed in transformed cell lines. Transient co-transfection of an EPR-1 cDNA potentially acting as a Survivin antisense with a lacZ reporter plasmid resulted in loss of viability of HeLa cells. In contrast, co-transfection of an antisense cDNA of intercellular adhesion molecule-1 or a sense-oriented Survivin cDNA was without effect. In stably transfected HeLa cells, ZnSO4 induction of an EPR-1 mRNA under the control of a metallothionein promoter suppressed the expression of endogenous survivin. This resulted in (i) increased apoptosis as detected by analysis of DNA content and in situ internucleosomal DNA fragmentation and (ii) inhibition of cell proliferation as compared with induced vector control transfectants. These findings suggest the existence of a potential EPR-1/survivin gene cluster and identify survivin as a new target for disrupting cell viability pathways in cancer.


Subject(s)
Apoptosis/genetics , Cell Division/genetics , Gene Targeting , Microtubule-Associated Proteins , Proteins/genetics , Chromosome Mapping , Chromosomes, Human, Pair 17 , DNA Fragmentation , Down-Regulation , HeLa Cells , Humans , Inhibitor of Apoptosis Proteins , Neoplasm Proteins , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Cell Surface/genetics , Survivin
17.
DNA Seq ; 8(5): 317-27, 1998.
Article in English | MEDLINE | ID: mdl-10993602

ABSTRACT

In numerous population genetic and disease association studies decisions about the ancestry of polymorphic alleles are often made based on the relative frequency of the alleles in the extant populations with the most frequent allele being deemed as ancestral. However, the frequency of an allele in a population is generally not a perfect indicator of its ancestral status. A more accurate method to assess ancestral/derived status of polymorphic alleles involves identification of shared alleles between species. We used this strategy to examine genomic regions homologous to several human polymorphisms in four species of non-human primates. Cross species polymerase chain reaction (CS-PCR), with primers designed from human sequence, was used to investigate regions of interest. Nineteen polymorphisms at six loci (DRD2, HOXB@, PAH, D4S10, RBP3, and RET) were examined either by restriction fragment length analysis of PCR products (PCR-RFLP) or by direct sequencing. At seventeen of the eighteen PCR-RFLPs, non-human primates were monomorphic and identical to each other for either lack of restriction enzyme site or presence of the site. Thus, at these seventeen polymorphic sites the shared alleles are most likely to be the ancestral ones in humans. In several cases we have used sequence data to further demonstrate that the nucleotide at the site of the polymorphism is conserved between species confirming the hypothesis of a single ancestral allele. However, not all human alleles can be simply resolved into ancestral and derived; sequence data from one PCR-RFLP (in an intron of the PAH locus) and a single strand conformational polymorphism (SSCP) in the 3' untranslated region (UTR) of the DRD2 gene illustrate this point.


Subject(s)
Alleles , Evolution, Molecular , Polymorphism, Genetic , Animals , Gene Frequency , Gorilla gorilla/genetics , Humans , Pan paniscus/genetics , Pan troglodytes/genetics , Polymerase Chain Reaction/methods , Polymorphism, Restriction Fragment Length , Polymorphism, Single-Stranded Conformational , Pongo pygmaeus/genetics , Sequence Analysis, DNA/methods , Species Specificity
19.
Hum Mol Genet ; 6(11): 1855-63, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9302263

ABSTRACT

There are currently 13 diseases known to be caused by unstable triplet repeat mutations; however, there are some instances (as with FRAXF and FRA16) when these mutations appear to be asymptomatic. In a search for polymorphic CTG repeats as candidate genes for bipolar disorder, we screened a genomic human chromosome 18-specific library and identified a 1.6 kb clone (7,6A) with a CTG24 repeat that maps to 18q21.1. The CTG repeat locus, termed CTG18.1, is located within an intron of human SEF2-1, a gene encoding a basic hellx-loop-hellx DNA binding protein involved in transcriptional regulation. The CTGn repeat is highly polymorphic and very enlarged alleles, consistent with expansions of up to CTG2100, were identified. PCR and Southern blot analysis in pedigrees ascertained for a Johns Hopkins University bipolar disorder linkage study and in CEPH reference pedigrees revealed a tripartite distribution of CTG18.1 alleles with stable alleles (CTG10-CTG37), moderately enlarged and unstable alleles (CTG53-CTG250), and very enlarged, unstable alleles (CTG800-CTG2100). Moderately enlarged alleles were not associated with an abnormal phenotype and have a combined enlarged allele frequency of 3% in the CEPH and bipolar populations. Very enlarged alleles, detectable only by Southern blot analysis of genomic digests, have thus far been found in only three individuals from our bipolar pedigrees, and to date, have not been found in any of the CEPH reference pedigrees. These enlarged alleles may arise, at least in part, via somatic mutation.


Subject(s)
Chromosomes, Human, Pair 18 , DNA-Binding Proteins/genetics , Introns , Trans-Activators/genetics , Transcription Factors/genetics , Trinucleotide Repeats , Alleles , Base Sequence , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors , Bipolar Disorder/genetics , Blotting, Southern , Cell Line , Cloning, Molecular , Female , Gene Frequency , Helix-Loop-Helix Motifs/genetics , Humans , Male , Molecular Sequence Data , Pedigree , Sequence Analysis , TCF Transcription Factors , Transcription Factor 4 , Transcription Factor 7-Like 2 Protein
20.
Hum Mol Genet ; 6(3): 403-8, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9147643

ABSTRACT

Repeat Expansion Detection (RED) is an efficient and simple method for detecting repeat expansions in the human genome, including expansion mutations resulting in disease. Here we report the first population survey of CTG/CAG repeat lengths in humans using the RED method; we have determined maximum CTG/CAG repeat length in 244 individuals from six human populations: Danes, Chinese, Japanese, Rondonian Surui, Maya and Mbuti/Biaka Pygmies. We have also sampled a number of non-human primates including eight orang-utans (Pongo pygmaeus), seven gorillas (Gorilla gorilla), seven pygmy chimpanzees (Pan paniscus), 13 common chimpanzees (Pan troglodytes) and three Hylobatidae (one Hylobates lar, one H.klossii, and one H.syndactylus). Our results demonstrate the existence of significant variation in the sizes and frequencies of the longest CTG/CAG repeat length seen per individual both within and between human populations. The population differences argue that overall mutation rates at CTG/CAG repeat loci are sufficiently low that mutation does not obliterate the effect of random genetic drift and clearly indicate that population stratification could occur in disease association studies using the RED method. No significant differences were detected among the non-human primates sampled. Our results also show that both common chimpanzees and pygmy chimpanzees (bonobos) are polymorphic for maximum length of any CTG/CAG repeats while no variation was found for gorillas and orang-utans.


Subject(s)
DNA Mutational Analysis/methods , Genome, Human , Genome , Haplorhini/genetics , Trinucleotide Repeats/genetics , Animals , DNA Primers/genetics , Electrophoresis, Polyacrylamide Gel , Genetics, Population , Humans , Mutation/genetics , Polymorphism, Genetic/genetics
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