Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 10 de 10
Filter
1.
Tuberculosis (Edinb) ; 136: 102254, 2022 09.
Article in English | MEDLINE | ID: mdl-36126496

ABSTRACT

In our laboratory, Mycobacterium caprae has poor growth in standard medium (SM) 7H9-OADC supplemented with pyruvate and Tween-80. Our objectives were to identify mutations affecting M. caprae metabolism and use this information to design a culture medium to improve its growth. We selected 77 M. caprae genomes and sequenced M. caprae NLA000201913 used in our experiments. Mutations present in >95% of the strains compared to Mycobacterium tuberculosis H37Rv were analyzed in silico for their deleterious effects on proteins of metabolic pathways. Apart from the known defect in the pyruvate kinase, M. caprae has important lesions in enzymes of the TCA cycle, methylmalonyl cycle, B12 metabolism, and electron-transport chain. We provide evidence of enzymatic redundancy elimination and epistatic mutations, and possible production of toxic metabolites hindering M. caprae growth in vitro. A newly designed SM supplemented with l-glutamate allowed faster growth and increased final microbial mass of M. caprae. However, possible accumulation of metabolic waste-products and/or nutritional limitations halted M. caprae growth prior to a M. tuberculosis-like stationary phase. Our findings suggest that M. caprae relies on GABA and/or glyoxylate shunts for in vitro growth in routine media. The newly developed medium will improve experiments with this bacterium by allowing faster growth in vitro.


Subject(s)
Mycobacterium bovis , Mycobacterium tuberculosis , Genomics , Glutamic Acid , Glyoxylates , Mycobacterium bovis/genetics , Mycobacterium tuberculosis/genetics , Polysorbates , Pyruvate Kinase , Pyruvates , gamma-Aminobutyric Acid
2.
Microbiol Resour Announc ; 10(39): e0052121, 2021 Sep 30.
Article in English | MEDLINE | ID: mdl-34591668

ABSTRACT

Alcaligenes faecalis is a Gram-negative rod that is ubiquitous in the environment and is an opportunistic human pathogen. Here, we report the whole-genome sequencing analysis of A. faecalis HZ01, which presents mycobacterial growth inhibitory activity and was isolated from a contaminated culture of Mycobacterium chubuense ATCC 27278.

3.
Mem Inst Oswaldo Cruz ; 116: e200517, 2021.
Article in English | MEDLINE | ID: mdl-33729319

ABSTRACT

Molecular-typing can help in unraveling epidemiological scenarios and improvement for disease control strategies. A literature review of Mycobacterium tuberculosis transmission in Brazil through genotyping on 56 studies published from 1996-2019 was performed. The clustering rate for mycobacterial interspersed repetitive units - variable tandem repeats (MIRU-VNTR) of 1,613 isolates were: 73%, 33% and 28% based on 12, 15 and 24-loci, respectively; while for RFLP-IS6110 were: 84% among prison population in Rio de Janeiro, 69% among multidrug-resistant isolates in Rio Grande do Sul, and 56.2% in general population in São Paulo. These findings could improve tuberculosis (TB) surveillance and set up a solid basis to build a database of Mycobacterium genomes.


Subject(s)
Minisatellite Repeats/genetics , Mycobacterium tuberculosis/genetics , Polymorphism, Restriction Fragment Length/genetics , Bacterial Typing Techniques , Brazil/epidemiology , Genotype , Humans , Molecular Epidemiology , Mycobacterium tuberculosis/isolation & purification , Whole Genome Sequencing
4.
Infect Drug Resist ; 14: 459-466, 2021.
Article in English | MEDLINE | ID: mdl-33574685

ABSTRACT

PURPOSE: Mycobacterium bovis Bacillus Calmette-Guérin (BCG) is the only vaccine licensed against tuberculosis. Despite the protection offered by the vaccine, in some circumstances children and immunocompromised individuals can develop associated infections, known as BCGitis. Drug susceptibility patterns of BCG clinical strains have rarely been described. We aimed to describe the susceptibility pattern of BCG clinical strains isolated in two different countries. METHODS: We performed culture-based drug susceptibility testing of thirty one BCG strains isolated from patients in Brazil (n=5, 16%) and Argentina (n=26, 84%) using the broth micro-dilution method (phenotypic method). Final antibiotic concentrations for susceptibility testing ranged from 0.5 to 16 mg/L for amikacin, 0.3125 to 10 mg/L for ethambutol, 0.05 to 1.6 mg/L for isoniazid and 0.25 to 8 mg/L for rifampicin, streptomycin and ofloxacin. Additionally, we compared the results with genetic data obtained by whole genome sequencing. RESULTS: By using the phenotypic method we detected one strain resistant to ethambutol, three strains resistant to rifampicin and one resistant to isoniazid. Additionally, two strains that were phenotypically resistant to both isoniazid and rifampicin carried mutations in the katG and rpoB genes simultaneously. CONCLUSION: There is evidence of the emergence of BCG-resistant strains isolated from vaccine-related complications. We recommend drug susceptibility testing of the BCG strain causing the infection in order to prevent treatment failure.

5.
Infect Genet Evol ; 85: 104550, 2020 11.
Article in English | MEDLINE | ID: mdl-32920193

ABSTRACT

Human tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis complex (MTBC), including Mycobacterium tuberculosis var. tuberculosis (MTB) and Mycobacterium tuberculosis var. africanum (MAF). While MTB is isolated worldwide, MAF is almost completely restricted to the African continent, and despite the historical proximity between Brazil and Africa during the slave trade, no case of TB being caused by MAF has been reported in Brazil to date. We hereby describe the first case of TB caused by MAF in Brazil comparing its genome against the published ones. A female patient who had never visited Africa presented with clinical symptoms typical of pulmonary TB. Based on 16S rRNA gene sequencing, the cultured isolate was identified as belonging to MTBC and partial sequence of the hsp65 gene was identical to that of MAF. This was confirmed by genotyping based on detection of Single Nucleotide Polymorphism (SNP), Region of Difference (RD) and spoligotyping. The isolate presented the Shared International Typing (SIT) 181. In the whole-genome comparison against MAF genomes available on published EMBL-EBI European Nucleotide Archive (ENA), the Brazilian genome (MAFBRA00707) was identified as belonging to Lineage 6 and clustered with isolates from The Gambia. This is the first report of the isolation of MAF from a patient from Brazil, without evidence of having any contact with an African index case.


Subject(s)
Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/genetics , Tuberculosis, Pulmonary/diagnosis , Tuberculosis, Pulmonary/microbiology , Brazil/epidemiology , Genes, Bacterial , Genome, Bacterial , Genotype , Humans , Molecular Epidemiology , Molecular Typing , Mycobacterium tuberculosis/isolation & purification , Phylogeny , Polymorphism, Single Nucleotide , RNA, Ribosomal, 16S , Tuberculosis, Pulmonary/epidemiology
6.
Mem Inst Oswaldo Cruz ; 115: e190401, 2020.
Article in English | MEDLINE | ID: mdl-32401897

ABSTRACT

Bacillus Calmette Guerin (BCG) vaccines comprise a family of related strains. Whole genome sequencing has allowed the better characterisation of the differences between many of the BCG vaccines. As sequencing technologies improve, updating of publicly available sequence data becomes common practice. We hereby announce the draft genome of four commonly used BCG vaccines in Brazil, Argentina and Venezuela.


Subject(s)
BCG Vaccine/genetics , Chromosome Mapping , Mycobacterium bovis/genetics , Argentina , Base Sequence , Brazil , Humans , Molecular Sequence Data , Polymorphism, Single Nucleotide , Venezuela
7.
Trop Med Int Health ; 22(4): 407-414, 2017 04.
Article in English | MEDLINE | ID: mdl-28072501

ABSTRACT

OBJECTIVE: To assess risk factors for nasopharyngeal carriage of potential pathogens in geographically isolated Warao Amerindians in Venezuela. METHODS: In this point prevalence survey, nasopharyngeal swabs were obtained from 1064 Warao Amerindians: 504 children aged 0-4 years, 227 children aged 5-10 years and 333 caregivers. Written questionnaires were completed to obtain information on demographics and environmental risk factors. Anthropometric measurements were performed in children aged 0-4 years. RESULTS: Carriage rates of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae and Moraxella catarrhalis were 51%, 7%, 1% and 13%, respectively. Crowding index, method of cooking and tobacco exposure were not associated with increased carriage. In multivariable analysis, an increase in height-for-age Z score (i.e. improved chronic nutritional status) was associated with decreased odds of S. pneumoniae colonisation (OR 0.76, 95% CI 0.70-0.83) in children aged 0-4 years. CONCLUSIONS: Better knowledge of demographic and environmental risk factors facilitates better understanding of the dynamics of colonisation with respiratory bacteria in an Amerindian population. Poor chronic nutritional status was associated with increased pathogen carriage in children <5 years of age. The high rates of stunting generally observed in indigenous children may fuel the acquisition of respiratory bacteria that can lead to respiratory and invasive disease.


Subject(s)
Carrier State , Gram-Negative Bacteria/growth & development , Growth Disorders/complications , Indians, South American , Nasopharynx/microbiology , Respiratory Tract Infections/etiology , Staphylococcus/growth & development , Adolescent , Adult , Body Height , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , Nutritional Status , Prevalence , Respiratory Tract Infections/microbiology , Risk Factors , Surveys and Questionnaires , Venezuela , Young Adult
9.
Vaccine ; 34(20): 2312-20, 2016 Apr 29.
Article in English | MEDLINE | ID: mdl-27036512

ABSTRACT

OBJECTIVE: To determine the impact of pre-vaccination nutritional status on vaccine responses in Venezuelan Warao Amerindian children vaccinated with the 13-valent pneumococcal conjugate vaccine (PCV13) and to investigate whether saliva can be used as read-out for these vaccine responses. METHODS: A cross-sectional cohort of 504 Venezuelan Warao children aged 6 weeks - 59 months residing in nine geographically isolated Warao communities were vaccinated with a primary series of PCV13 according to Centers for Disease Control and Prevention (CDC)-recommended age-related schedules. Post-vaccination antibody concentrations in serum and saliva of 411 children were measured by multiplex immunoassay. The influence of malnutrition present upon vaccination on post-vaccination antibody levels was assessed by univariate and multivariable generalized estimating equations linear regression analysis. RESULTS: In both stunted (38%) and non-stunted (62%) children, salivary antibody concentrations correlated well with serum levels for all serotypes with coefficients varying from 0.61 for serotype 3-0.80 for serotypes 5, 6A and 23F (all p < 0.01). Surprisingly, higher serum and salivary antibody levels were observed with increasing levels of stunting in children for all serotypes. This was statistically significant for 5/13 and 11/13 serotype-specific serum and saliva IgG concentrations respectively. CONCLUSION: Stunted Amerindian children showed generally higher antibody concentrations than well-nourished children following PCV13 vaccination, indicating that chronic malnutrition influences vaccine response. Saliva samples might be useful to monitor serotype-specific antibody levels induced by PCV vaccination. This would greatly facilitate studies of vaccine efficacy in rural settings, since participant resistance generally hampers blood drawing.


Subject(s)
Antibodies, Bacterial/blood , Growth Disorders/immunology , Nutritional Status , Pneumococcal Vaccines/administration & dosage , Saliva/chemistry , Antibodies, Bacterial/chemistry , Child, Preschool , Cross-Sectional Studies , Female , Humans , Immunoglobulin G/blood , Immunoglobulin G/chemistry , Infant , Linear Models , Male , Malnutrition/immunology , Pneumococcal Infections/prevention & control , Serogroup , Streptococcus pneumoniae/classification , Vaccines, Conjugate/administration & dosage , Vaccines, Conjugate/therapeutic use , Venezuela
10.
J Infect Dev Ctries ; 8(4): 469-73, 2014 Apr 15.
Article in English | MEDLINE | ID: mdl-24727513

ABSTRACT

INTRODUCTION: Serotype surveillance of Streptococcus pneumoniae is indispensable for evaluating the potential impact of pneumococcal conjugate vaccines. Serotyping by the standard Quellung reaction is technically demanding, time consuming, and expensive. A simple and economical strategy is multiplex PCR-based serotyping. We evaluated the cost effectiveness of a modified serial multiplex PCR (mPCR), resolving 24 serotypes in four PCR reactions and optimally targeting the most prevalent invasive and colonizing pneumococcal serotypes found in Venezuela. METHODOLOGY: A total of 223 pneumococcal isolates, 140 invasive and 83 carriage isolates, previously serotyped by the Quellung reaction and representing the 18 most common serotypes/groups identified in Venezuela, were serotyped with the adapted mPCR. RESULTS: The mPCR serotyped 76% of all the strains in the first two PCR reactions and 91% after four reactions, correctly identifying 17 serotypes/groups. An isolate could be serotyped with mPCR in less than 2 minutes versus 15 minutes for the Quellung reaction, considerably lowering labor costs. A restrictive weakness of mPCR was found for the detection of 19F strains. Most Venezuelan 19F strains were not typeable using the mPCR, and two 19F cps serotype variants were identified. CONCLUSIONS: The mPCR assay is an accurate, rapid, and economical method for the identification of the vast majority of the serotypes from Venezuela and can be used in place of the standard Quellung reaction. An exception is the identification of serotype 19F. In this setting, most 19F strains were not detectable with mPCR, demonstrating a need of serology-based quality control for PCR-based serotyping.


Subject(s)
Carrier State/microbiology , DNA, Bacterial/analysis , Pneumococcal Infections/microbiology , Streptococcus pneumoniae/isolation & purification , Cost-Benefit Analysis , Humans , Polymerase Chain Reaction/economics , Polymerase Chain Reaction/methods , Serogroup , Streptococcus pneumoniae/genetics , Time Factors , Venezuela
SELECTION OF CITATIONS
SEARCH DETAIL
...