ABSTRACT
Simian adenovirus 20 DNA was specifically cleavered by restriction endonucleases EcoRI, BamHI, XbaI and HindIII. The transformation activity of the DNA digest was investigated. BamHI, XbaI, and HindII DNA digests were able to transform the primary rat kidney cell culture (Wistar) as well as the native SV20 DNA. The transforming activity was revealed in a specific fragment of the viral DNA, obtained after the treatment of the DNA with BamHI (fragment B), with molecular weight 5.4 x 10(6) dalton. This fragment is located in the left end of the viral genome. The lack of cell transformation by the EcoRI-hydrolysate of viral DNA may serve a proof of the extremely left position of the oncogene in the viral genome, since of EcoRI-fragment chips off a fragment with molecular weight 3 x 10(5) dalton fr om the left side of DNA molecule.
Subject(s)
Adenoviridae/analysis , Adenoviruses, Simian/analysis , Carcinogens , Cell Transformation, Viral , DNA, Viral/analysis , Adenoviruses, Simian/genetics , Animals , Cell Transformation, Viral/drug effects , DNA Restriction Enzymes/pharmacology , DNA, Viral/genetics , Genes, Viral/drug effects , Rats , Rats, Inbred Strains , Virus CultivationABSTRACT
Cell transformation by the complete and the incomplete virus SV20 was investigated. To compare the oncogenic properties of human adenoviruses, two types of cell systems--the primary rat embryonic cell culture and the primary rat kidney cell culture--were used. The dependence of DNA concentration on transformation frequency was investigated. With the incomplete virus SV20, the transformation efficiency was more than that with the complete virus (16 and 1-3 percent, resp.). In addition, it has been shown that the primary rat kidney cell culture is more sensitive to transformation than the primary rat embryonic cells culture. The new-born Sirian hamsters being inoculated with virus SV20 DNA, the growth of a tumor being registered in the point of injection 120 days afterwards.
Subject(s)
Adenoviridae/pathogenicity , Adenoviruses, Simian/pathogenicity , Carcinogens , Cell Transformation, Viral , DNA, Viral/pharmacology , Adenoviruses, Simian/genetics , Animals , Animals, Newborn , Defective Viruses/genetics , Defective Viruses/pathogenicity , Embryo, Mammalian , Genes, Viral , In Vitro Techniques , Kidney , Rats , Rats, Inbred Strains , Virus CultivationABSTRACT
The effect of specific restriction endonuclease on the simian adenovirus SV20 DNA was studied. It was shown that endonucleases SalI, XbaI, EcoRI, BamHI, HindIII cleaved the viral DNA into 3, 4, 5, 5, 8 specific fragments respectively. The sequence of fragments (physical map) was determined and found to be B-C-A for enzyme SalI, C-D-B-A--for enzyme Xbal, E-A-C-D-B--for enzyme EcoRI, B-E-C-A-D--for enzyme BamHI and B-E-A-C-(GH)-D-F--for enzyme HindIII. The G-C content of specific fragments was studied. The "right"-"left" orientation of the physical map of the simian adenovirus 20 DNA based on the G-C content was made in respect with the nomenclature of human adenoviruses.
Subject(s)
Adenoviridae/analysis , Adenoviruses, Simian/analysis , DNA Restriction Enzymes , DNA, Viral , Deoxyribonucleases, Type II Site-Specific , Genes, Viral , Adenoviruses, Human , Base Sequence , Deoxyribonuclease BamHI , Deoxyribonuclease HindIII , Molecular Weight , Species Specificity , Substrate Specificity , Terminology as TopicABSTRACT
The effect of specific endonucleases on DNA chiken adenovirus CELO was studed. It was shown that endonucleases R. HpaI, R. EcoRI and R. HindIII cleaved viral DNA into 5,7 and 9 specific fragments, respectively. The sequence of fragments (physical map) was determined and found to be: D-A-E-C-B for enzyme R. HpaI; B-(EG)-C-A-D-F for enzyme R. EcoRI; H-F-A-C-G-B-D-E-I for enzyme R. HindIII.
Subject(s)
Adenoviridae/analysis , Aviadenovirus/analysis , DNA Restriction Enzymes , DNA, Viral , Base Sequence , DNA Restriction Enzymes/metabolism , Escherichia coli/enzymology , Haemophilus/enzymology , Oligodeoxyribonucleotides/analysis , Substrate SpecificityABSTRACT
The author demonstrated the transmission of chromosomal genes, controlling the histidine, proline, tryptophane synthesis, from Ps. aeruginosa to E. coli in conjugation mediated through the factor of drug resistance. A simultaneous transmission of the drug resistance and of the chromosomal genes by some transconjugants with further conjugation and also in transduction, a simultaneous transmission of the mentioned signs by transductants in conjugation with RecA--recipients, and also the presence of the satellite DNA peaks in the transconjugants permitted to draw a conclusion on the formation of the replaced RP factors, R'-his, R'-pro, and also R'-protrp.
