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Evaluation of BacT/Alert 3D automated unit for detection of nontuberculous mycobacteria requiring incubation at 30 degrees C for optimal growth.
Alfa, Michelle J; Manickam, Kanchana; Sepehri, Shadi; Sitter, Denise; Lenton, Pat.
J Clin Microbiol ; 49(7): 2691-3, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21543566

ABSTRACT

The reliability of the BacT/Alert 3D unit for automated detection of nontuberculous mycobacteria (NTM) that grow optimally at 30 °C was assessed. This system reliably maintained a temperature of 30 °C and detected 50% of the clinical NTM strains (5 Mycobacterium marinum and 3 Mycobacterium gordonae strains) faster than 37 °C culture.


Subject(s)
Automation/methods , Bacteriological Techniques/methods , Mycobacterium Infections/diagnosis , Mycobacterium marinum/classification , Mycobacterium marinum/isolation & purification , Nontuberculous Mycobacteria/classification , Nontuberculous Mycobacteria/isolation & purification , Humans , Mycobacterium marinum/growth & development , Nontuberculous Mycobacteria/growth & development , Sensitivity and Specificity , Temperature
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