ABSTRACT
This study was undertaken to identify the most effective dosage level of folltrooin for superovulation in buffalo. In addition, the effect of the day of estrus on superovulation and the use of exogenous GnRH were also studied. Eighty-three buffalo were treated with prostaglandin. A functional Corous luteum (CL) was palpated in only 73 buffalo 1 d before the superovulation treatment was initiated. One of eight treatments was used on the buffalo: Protocol I(n 8) 9 mg folltropin (PPFE) on Days 9 to 12 of the cycle; Protocol II(n 10) 18 mg PPFE on Days 9 to 12 of the cycle; Protocol III(n 9) 18 mg PPFE on Days 13 to 15 of the cycle; Protocol IV(n 9) 21.6 mg PPFE on Days 9 to 12 of the cycle; Protocol V(n 9) 21.6 mg PPFE with GnRH on Days 9 to 12 of the cycle; Protocol-VI(n 10) 25.2 mg PPFE on Days 9 to 12 of the cycle; Protocol VII(n 9) 28.8 mg PPFE on Days 9 to 12 of the cycle; Protocol VIII (n 9) 36 mg PPFE on Days 9 to 12 of the cycle. The highest ovulation rate was observed in Protocol VI (x 5.3+/-0.79) which is significantly higher (P < 0.01) than other Protocols. Maxium embryos (x 3.7) were recovered using Protocol III. Whereas, highest number of transferable embryos (x 2.2) were recovered from Protocol V. Use of GnRH and superovulation treatment on Days 13 to 15 has no advantageous effect on ovulation rate. In all, 41 embryos were transferred to 35 recioients: nine buffalo became pregnant; 59 embryos were frozen; 12 were thawed; nine good frozen-thawed embryos were transferred to eight recidients, three of which were diagnosed pregnant.
