Enhanced applications in dentistry through autoclave-assisted sonochemical synthesis of Pb/Ag/Cu trimetallic nanocomposites.

In recent years, researchers have increasingly focused on the development of multiphase trimetallic nanocomposites (TMNC) incorporating ternary metals or metal oxides, which hold significant potential as alternatives for combatting biofilms and bacterial infections. Enhanced oral health is ensured by the innovative techniques used to effectively prevent bacterial adherence and formation of biofilm on dental sutures. In this investigation, TMNC, which consists of Pb, Ag, and Cu, was synthesized using an autoclave-assisted sonochemical technique. Following synthesis, TMNC were characterized using FTIR, XRD, BET, XPS, TGA, and Raman spectroscopy to analyze their shape and microstructure. Subsequent evaluations, including MTT assay, antibacterial activity testing, and biofilm formation analysis, were conducted to assess the efficiency of the synthesized TMNC. Cytotoxicity and anti-human oral squamous cell carcinoma activities of TMNC were evaluated using the Human Oral Cancer cell line (KB) cell line through MTT assay, demonstrating a dose-dependent increase in anti-human oral squamous cell carcinoma activity against the KB cell line compared to the normal cell line, resulting in notably high cell viability. Furthermore, an ultrasonic probe was employed to incorporate TMNC onto dental suturing threads, with different concentrations of TMNC, ultrasonic power levels, and durations considered to determine optimal embedding conditions that result in the highest antibacterial activity. The inhibitory effects of TMNC, both in well diffusion assays and when incorporated into dental suturing threads, against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria on Mueller-Hinton agar (MHA) were assessed using various concentrations of TMNC. The results of the study indicated that the efficacy of TMNC in inhibiting bacterial growth on dental suturing threads remained impressive, even at low concentrations. Moreover, an evaluation of their potential to destabilize biofilms formed by S. aureus and E. coli, the two pathogens in humans, indicated that TMNC would be a promising anti-biofilm agent.

An eco-friendly ultrasound approach to extracting yellow dye from Cassia alata flower petals: Characterization, dyeing, and antibacterial properties.

Using natural dyes in dyeing industries becomes an alternative to synthetic dyes, which are known to contain harmful chemicals that can pose risks to the environment and human health. This study involves the extraction of yellow dye from Cassia alata flower petals, optimization of the extraction process using an ultrasonic bath (40 KHz and an input power of 500), ultrasonic probe (390 W, 455 W, 520 W, 585 W, and 650 W), and conventional heating (heating mantle with 30 °C, 40 °C, 50 °C, 60 °C, and 70 °C), characterization of the dye, as well as dyeing (cotton, silk, and leather) without using a mordant. The extracted yellow dye was further evaluated to determine its antibacterial activity against skin bacteria. Dye extraction optimization using UV-Visible spectrophotometric analysis revealed that the maximum yellow color in methanol extract (287 and 479 nm) was obtained at 50 °C for 45 min using ultrasonic water bath extraction, followed by the ultrasonic probe and direct heating. Based on the FTIR spectra, it is evident that OH is present at approximately 3300 cm-1, while CH stretches at around 2900 cm-1. A characteristic peak at 1608 cm-1 bears a striking similarity to anthraquinonoid-based compounds. Also, using the ultrasonic water bath dyeing technique at 50 °C for 45 min, the yellow color of cotton, silk, and leather was dyed optimally. Due to effective color removal after two washings with boiling soap liquid, the dyed cotton and silk fabric displayed good washing and rubbing fastness. Regarding antibacterial activity, the dye was highly active against all pathogens after extraction in methanol. The maximum inhibition was observed against Pseudomonas sp. with a MIC value of 1.56 mg/ml.

Occurrence, morphology and molecular characterisation of Bopyrid parasite Epipenaeon ingens Nobili, 1906 (Isopoda: Bopyridae).

Epipenaeon ingens occurs most commonly in Southeast Coast of India parasitized the penaeid shrimp. The present study provides the prevalence, morphological and molecular characterisation of E. ingens collected from the host Metapenaeopsis stridulans. In the present study highlights the significance of high prevalence and genetic diversity of E. ingens in M. stridulans. Intra specific analysis also revealed that E. ingens species detected in the present study can be easily distinguished from the other genospecies of bopyrids. Further application of this molecular tool to investigate the genetic variability among bopyrids detected in different hosts may facilitate our understanding of the significance of genetic diversity in relation to the infestation of bopyrid species in south coast of India.

High prevalence and infestation of Mothocya renardi (Isopoda, Cymothoidae) in marine fish Strongylura leiura (Bleeker 1850).

Cymothoid isopods are parasites that cause severe damage to the fish species. The present study was focused to carry out the parasitic infestation on the one of the important marine fish Strongylura leiura during the 2 years period between June 2009 and May 2011. 272 fish specimens collected and about 157 (57.72 %) fishes were infested by the cymothoid which were concealed under the gill arches of the host. Infested parasite was identified as Mothocya renardi. The prevalence rate of parasites during different seasons of the range of host specificity of isopods was studied. Variation of infestation rate also depends on the size, length and weight of the host. The sites of infestation by the parasitic crustaceans are highly specific. The infestation had affected the normal growth of the host fish and also probably leads the high levels of secondary infections.

Unusual attachment of Nerocila exocoeti in Hemiramphus far (Forsskal, 1775) from Parangipettai south east coast of India.

A female Nerocila exocoeti was found attached on the head region of the halfbeak fish Hemiramphus far reported for the first time from Parangipettai south east coast of India. This was abnormal position to all known cymothoids attached on the surface of host fishes. This isopod naturally occurs in the fish belongs to Exocoetidae. The attachment must have occurred accidentally. This is only the second time this isopod has been collected in the south coast India and the first for this host.

A new record of parasitic isopod for the Indian fauna (Mothocya karobran Bruce, 1986) from Strongylura strongylura in the Pazhayar region, Southeast coast of India.

Cymothoid isopods are permanent ectoparasites of fish. In the present study Strongylura strongylura captured in gillnets from the inshore waters of Pazhayar from January to March-12. Among the 122 Belonidae fishes examined, 16 specimens were parasitized by isopods belonging to the species Mothocya karobran, a new record of this cymothoid isopod for the first time from India. Lowest prevalence occurred in January (10.5 %) and the highest in February (15.9 %). The infected fishes usually carried one cymothoid per branchial cavity. 12 parasites were found in the left branchial cavity and 4 parasites in the right. The non ovigerous female (18.5-24.7 mm) and female ovigerous (20.8-27.4 mm) in total length.

New occurrence of parasitic isopods from Indian fishes.

Until now, 36 species belonging to the family Cymothoidae (Crustacea, Isopoda) were recorded from Indian fishes. In this study, ten additional cymothoids are reported in India, most of them for the first time. They parasitize nine fish species, several of them being new host species.

Preliminary studies for a new antibiotic from the marine mollusk Melo melo (Lightfoot, 1786)

OBJECTIVE@#To search for new bioactive compounds from marine mollusks Melo melo (M. melo).@*METHODS@#Preliminary work for bioactive compound was identified by using disc diffusion methods against human pathogens. Further analyses of compound were done by using TLC, SDS-PAGE. And also estimate the amount of protein in the samples by following Biuret method.@*RESULTS@#In antibacterial activity the maximum diameter of 24 mm zone of inhibition was recorded against Klebsiella pneumoniae (K. pneumoniae) strain of the mucus extract and minimum zone of inhibition of 11 mm was observed in Salmonella typhi (S. typhi) strain of body tissue extract. The antifungal activity of the extraction shows maximum activity against Trichophyton mentagarophytes (T. mentagarophytes) (14 mm) and minimum activity was recorded in Aspergillus flavus (A. flavus) (11 mm). The extract of mucus, nerve tissue, body tissue and kidney that showed antimicrobial activity was subjected to TLC to determine the presence of the peptides and amide groups, and also subjected to SDS-PAGE to estimate the molecular weight of proteins in a clear band were detected in the gel that represented kidney, body tissue, brain and mucus represent 14, 17, 22, 45 kDa.@*CONCLUSIONS@#The extracts from marine mollusks M. melo is the potential source of producing bioactive compounds against human pathogens and can be used for synthesis of new drugs.