ABSTRACT
Polymorphism of chs_H1 gene encoding the "true" chalcone synthase was determined by alignment of sequences. The polymorphism associates with single nucleotide changes, insertions or deletions (indels) in the promoter, exons, intron, 3'-untranslated region. The molecular-genetic polymorphism in gene chs_H1 different regions of hop varieties of Polessye Agriculture Institute' breeding NAAS was analyzed.
Subject(s)
Acyltransferases/genetics , Genes, Plant , Humulus/genetics , INDEL Mutation , Polymorphism, Single Nucleotide , 3' Untranslated Regions , Amino Acid Sequence , Exons , Gene Expression , Humulus/enzymology , Introns , Molecular Sequence Data , Promoter Regions, Genetic , Sequence AlignmentABSTRACT
The collections of varieties of spring barley cultivars from the Eastern European and Central Asian areas were analyzed by exonpecific PCR (EPIC) for beta-amylase genes. The endosperm beta-amylase gene (bamyl) was differentiated by the presence of 126 bp MITE insertion into intron 3 that is associated with low activity beta-amylase. The findings suggest that a low level of genetic variation for bamylgene within climatic zones is associated with individual breeding program for each climatic zone.
