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1.
Ukr Biochem J ; 88(5): 45-51, 2016.
Article in English | MEDLINE | ID: mdl-29235835

ABSTRACT

At higher order levels chromatin fibers in interphase nuclei are organized into loop domains. Gene regulatory elements (promoters and enhancers) are often located near the sites of loop attachments. Therefore, loop domains play a key role in regulation of cell transcriptional activity. We investigated the kinetics of DNA loop exit during single cell gel electrophoresis (the comet assay) of nucleoids obtained from two cell types that differ in their synthetic activity ­ human lymphocytes and lymphoblasts. Lymphocyte activation and transformation into lymphoblasts (blast transformation) was performed with interleukin 2. The results obtained suggest that a rearrangement of the loops occurs after lymphocyte activation. After blast transformation we observed an increase of the amount of loop domains on the surface of nucleoids against a decrease of the inner loop fraction. Therefore, the comet assay can be used for detection of large-scale changes in the cell nucleus that follow changes in cell functional state.


Subject(s)
Cell Transformation, Neoplastic/genetics , Chromatin/chemistry , DNA/chemistry , Interleukin-2/pharmacology , Lymphocytes/drug effects , Cell Nucleus/metabolism , Cell Nucleus/ultrastructure , Cell Transformation, Neoplastic/metabolism , Cell Transformation, Neoplastic/pathology , Chromatin/metabolism , Comet Assay , DNA/metabolism , Humans , Kinetics , Lymphocyte Activation , Lymphocytes/metabolism , Lymphocytes/pathology , Nucleic Acid Conformation , Primary Cell Culture , Single-Cell Analysis
2.
Ukr Biochem J ; 87(1): 91-8, 2015.
Article in English | MEDLINE | ID: mdl-26036135

ABSTRACT

The self-ordering of C60 fullerene, doxorubicin and their mixture precipitated from aqueous solutions was investigated using atomic-force microscopy. The results suggest the complexation between the two compounds. The genotoxicity of doxorubicin in complex with C60 fullerene (C 60+Dox) was evaluated in vitro with comet assay using human lymphocytes. The obtained results show that the C60 fullerene prevents the toxic effect of Dox in normal cells and, thus, C60+Dox complex might be proposed for biomedical application.


Subject(s)
Antineoplastic Agents/pharmacology , Doxorubicin/pharmacology , Drug Carriers/chemistry , Fullerenes/chemistry , Lymphocytes/drug effects , Antineoplastic Agents/chemistry , Cell Survival/drug effects , Comet Assay , DNA Fragmentation/drug effects , Doxorubicin/chemistry , Humans , Lymphocytes/cytology , Microscopy, Atomic Force , Mutagenicity Tests , Primary Cell Culture , Surface Properties
3.
Ukr Biochem J ; 86(4): 110-8, 2014.
Article in Russian | MEDLINE | ID: mdl-25509189

ABSTRACT

The main mechanism of DNA track formation during comet assay of nucleoids, obtained after removal of cell membranes and most of proteins, is the extension to anode of negatively supercoiled DNA loops attached to proteins, remaining in nucleoid after lysis treatment. The composition of these residual protein structures and the nature of their strong interaction with the loop ends remain poorly studied. In this work we investigated the influence of chloroquine intercalation and denaturation of nucleoid proteins on the efficiency of electrophoretic track formation during comet assay. The results obtained suggest that even gentle protein denaturation is sufficient to reduce considerably the effectiveness of the DNA loop migration due to an increase in the loops size. The same effect was observed under local DNA unwinding upon chloroquine intercalation around the sites of the attachment of DNA to proteins. The topological interaction (protein intercalation into the double helix) between DNA loop ends and nucleoid proteins is discussed.


Subject(s)
Chloroquine/chemistry , Chromatin/chemistry , DNA/chemistry , Intercalating Agents/chemistry , Lymphocytes , Cells, Cultured , Comet Assay , Humans , Nucleic Acid Conformation , Protein Denaturation
4.
Tsitol Genet ; 43(6): 3-7, 2009.
Article in Russian | MEDLINE | ID: mdl-20458971

ABSTRACT

The results are presented on comparison of the kinetics of DNA exit during neutral and alkaline variants of single cell gel electrophoresis. It has been shown that pre-incubation of samples in alkaline buffer makes impossible DNA exit during the neutral electrophoresis, in contrast to the alkaline conditions where DNA exit is very efficient. The conclusion has been made that the alkaline conditions induce a disruption of DNA matrix interactions. The hypothetical nature of these interactions is discussed. The results obtained suggest that the mechanisms of DNA exit in the course of neutral and alkaline electrophoresis are essentially different. In the case of the neutral electrophoresis the comet tails are formed by the relaxed loop domains while during the alkaline electrophoresis they are formed by single stranded DNA fragments, which are pulled out by one and from the coil that is not bound to the matrix.


Subject(s)
Comet Assay/methods , DNA Breaks, Double-Stranded , DNA Breaks, Single-Stranded , DNA/analysis , DNA/genetics , Humans , Hydrogen-Ion Concentration , Lymphocytes/metabolism , Sensitivity and Specificity
5.
Mol Biol (Mosk) ; 36(3): 391-6, 2002.
Article in Russian | MEDLINE | ID: mdl-12068622

ABSTRACT

The minireview presents recent results obtained in the experiments with DNA minicircles containing reconstituted nucleosomes. This system allows one to register and to characterize conformational dynamics of nucleosomes and of subnucleosomal particles containing histone tetramer (H3-H4)2. In particular, it has revealed an important role of the histone N-terminal tails in this dynamics. Solution of the linking number paradox and relevance of the results obtained to chromatin structural dynamics are discussed.


Subject(s)
Nucleosomes/chemistry , DNA/chemistry , Histones/chemistry , Nucleic Acid Conformation
6.
Biochim Biophys Acta ; 1351(1-2): 213-22, 1997 Mar 20.
Article in English | MEDLINE | ID: mdl-9116035

ABSTRACT

The influence of ionic strength on DNA-histone and histone-histone interactions in reconstituted nucleosomes was studied by measuring the parameters of histone tyrosine fluorescence: fluorescence intensity and lambda(max) position. The first parameter is sensitive to histone-DNA interactions. The changes of the second one accrue due to hydrogen bond formation/disruption between tyrosines in the histone H2A-H2B dimer and the (H3-H4)2 tetramer. The simultaneous measurement of these parameters permits the recording of both the dissociation of histone complexes from DNA, as well as changes in histone-histone interactions. As ionic strength is increased, the H2A-H2B histone dimer dissociated first, followed by dissociation of the (H3-H4)2 tetramer [Yager, T.G., McMurray, C.T. and Van Holde, K.E. (1989) Biochemistry 28, 2271-2276]. The H2A-H2B dimer is dissociated in two stages: first, the ionic bonds with DNA were disrupted, followed by the dissociation of the histone dimer from the tetramer. And secondly, the disruption of dimer-tetramer specific H-bonds. It was established that the energy of electrostatic interactions of the histone dimer with DNA within the nucleosome is much less than the energy of interaction of the histone dimer with the tetramer.


Subject(s)
DNA-Binding Proteins/metabolism , DNA/metabolism , Histones/metabolism , Nucleosomes/metabolism , Dimerization , Models, Chemical , Models, Structural , Nucleosomes/drug effects , Osmolar Concentration , Protein Binding , Protein Conformation , Sodium Chloride/pharmacology , Spectrometry, Fluorescence , Thermodynamics , Tyrosine/chemistry
7.
J Mol Biol ; 247(5): 918-31, 1995 Apr 14.
Article in English | MEDLINE | ID: mdl-7723041

ABSTRACT

A model has been derived that accounts for the nucleosome translational position in terms of the bending free energy that depends on the nearest-neighbor interactions between base-pairs. The available data on the nucleosome positioning on defined DNA sequences in the reconstituted systems have been analyzed. It has been shown that the model allows one to predict the preferred nucleosome translational positioning with an accuracy of about one turn of the double helix. The conclusion is made that the isotropic elastic properties of the DNA molecule are very important for nucleosome translational positioning. The anisotropic flexibility modulates the sequence-dependent preference and defines the precise rotational placement. The analysis points to a possible involvement of DNA bendability in nucleosome structural transitions. To model the nucleosome positioning within the chromatin fiber, the derived algorithm has been applied to random DNA sequences. The nucleosome distribution obtained is close to random, but nucleosomes, according to calculations, are placed on sites with a low value of bending free energy and decreased G+C-content. Relations with other work and some implications are discussed.


Subject(s)
Chromatin/chemistry , DNA/chemistry , Nucleic Acid Conformation , Nucleosomes/chemistry , Animals , Base Composition , Chickens/genetics , Computer Simulation , DNA, Ribosomal/chemistry , Erythrocytes/chemistry , Models, Molecular , Plasmids/chemistry , Probability , RNA, Ribosomal, 5S/genetics , Sea Urchins/chemistry
8.
Article in Russian | MEDLINE | ID: mdl-1302503

ABSTRACT

Two fractions of rat liver nuclei with different buoyant density have been obtained. The electrophoretic analysis of the oligonucleosome patterns of DNA out of nuclei of these two fractions revealed different levels of activity in endonucleases. In case of inhibition during the extraction of activity in Ca, Mg-dependent endonucleases, the average size of high polymeric DNA is larger for nuclei with bigger buoyant density (fraction I) than for nuclei with smaller ones (fraction II). This finding is evidence of in situ existence of two pools of liver nuclei with different endogenic nuclease activities. In nuclear chromatin fraction I DNA is torsionally stressed; in fraction II it is relaxed that correlates with larger activity of endonucleases and smaller buoyant density of this fraction. A hypothesis on a possible role of endonucleases in chromatin structure organization has been put forward. According to this hypothesis a modulation of activity in nuclear endonucleases can determine different packaging and activity of chromatin from different pools of cellular nuclei.


Subject(s)
Chromatin/chemistry , Deoxyribonucleases/chemistry , Animals , Cell Fractionation/methods , Cell Nucleus/chemistry , DNA/analysis , DNA/isolation & purification , Deoxyribonucleases/analysis , Liver/chemistry , Rats , Rats, Wistar , Spectrometry, Fluorescence/methods
9.
Mol Biol (Mosk) ; 25(1): 144-52, 1991.
Article in Russian | MEDLINE | ID: mdl-1654518

ABSTRACT

The circular DNA which contains nucleosomes and additional supercoils has been considered theoretically. The different possible effect of increased negative supercoiling on the nucleosome structure have been studied. According to the model proposed all supercoils in the nucleosome-containing circular DNA are realized as torsional deformations of the double helix. The free energy of both supercoiling (torsional deformations) and nucleosome stabilization have been taken into consideration to obtain the equation for free energy of nucleosome-containing circular DNA. The analysis of this equation and the experimental data by Garner et al. (II Psoc. Natl. Acad. Sci. USA. 1987. P. 2620-2623) about the maximum amount of supercoiling obtained by DNA-topoisomerase II treatment of nucleosome-containing pBR322 plasmid has been performed. It has been shown that two possibilities are consistent with both the equation and experimental data. These are: (1) the increased supercoiling induces the torsional strains not only in linker regions but also in nucleosome DNA and thus supercoiling causes an instability on nucleosome structure; (2) increased supercoiling induces a structural change of nucleosome which is accompanied by nucleosome DNA unwinding and its transition into form with approximately 11 base pairs per turn of double helix. It has been evaluated that in the first case the average torsional rigidity of nucleosome DNA should be approximately 2.5 times as much and in the second case--much more than the rigidity of naked DNA. Both types of nucleosome structural changes may cause its transition to a potentially active state for transcription. It is suggested that increased supercoiling can be a switch mechanism of chromatin activation.


Subject(s)
DNA, Superhelical/chemistry , Nucleosomes/chemistry , Base Composition , Chromatin , DNA Topoisomerases, Type I/chemistry , Nucleic Acid Hybridization , Transcription, Genetic
10.
Biofizika ; 34(1): 28-33, 1989.
Article in Russian | MEDLINE | ID: mdl-2730930

ABSTRACT

DNA compactization in the presence of polycationic ligands was analysed theoretically. The concept is substantiated which states that the formation of polycationic bridges between the DNA regions adjoining in the chain is the principal mechanism of compactization. A phase diagram is plotted for DNA transition into the compact state in the coordinates of solution ionic strength and ligand concentration. The ligand binding with DNA under compactization conditions is shown to be characterized by positive cooperativity. DNA compactization by protamines and histones HI is discussed in terms of the results obtained.


Subject(s)
DNA/drug effects , Nucleic Acid Conformation , Polyamines , Polymers/pharmacology , Mathematics , Models, Theoretical , Polyelectrolytes
11.
Mol Biol (Mosk) ; 22(2): 414-22, 1988.
Article in Russian | MEDLINE | ID: mdl-3393151

ABSTRACT

The electrostatic interaction of extended cationic ligands with DNA has been considered on the basis of the analytical solution of a simplified Poisson--Boltzmann equation for the charged polyion cylinder. The control numerical solution of rigorous Poisson--Boltzmann equation shows that the assumption about the absence of coions in the vicinity of the highly charged polyion cylinder does not significantly influence the accuracy of solution and DNA electrostatic free energy evaluation. It was found that the basic contribution to the free energy of electrostatic ligand-DNA interaction is the mixing entropy change due to release of counterions from the vicinity of DNA. The equation for the dependence of the ligand to DNA binding constant K upon ionic strength c has been derived without introduction of any empirical parameters. This equation is consistent with the experimental data and can be used for the determination of a number of ligand--DNA ionic contacts in a wide range of salt concentrations. The main consequences of Manning and Record et al. theories can be considered as limiting cases of the theory presented. In particular the equation d(lnK)/d(lnc) = -0.88 N by Record et al. has a restricted range of application and it can be used only for a relative approximate estimation of the number of electrostatic bonds in ligand-DNA complexes. The analysis of electrostatic interaction of DNA with ligands which neutralize only part of phosphate groups in the binding site of DNA was also performed.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
DNA/metabolism , Ligands/metabolism , Binding Sites , Cations , Electric Conductivity , Mathematics , Models, Biological , Osmolar Concentration
12.
Mol Biol (Mosk) ; 21(3): 714-23, 1987.
Article in Russian | MEDLINE | ID: mdl-3657772

ABSTRACT

The theoretical analysis of nucleosome stability at low ionic strength has been performed on the basis of consideration of different contributions to the free energy of compact state of the nucleosome DNA terminal regions. The proposed model explains: the fact of low-salt structural change; the transition point (approximately 1.7 mM NaCl) and width (approximately 1 mM); the shift of the transition to the higher salt concentrations in the case of histones tails removal by trypsin. According to the model the increase of electrostatic repulsion between neighbouring turns of DNA superhelix is the main cause of the unwinding of nucleosomal DNA terminal regions in the course of low-salt structural change. The interactions between histone (H2A-H2B) dimer and (H3-H4)2 tetramer provide the compact state of the nucleosomal DNA terminal regions. The existence of electrostatic interactions of nucleosomal DNA terminal regions with tetramer was suggested. These interactions can provide the compact state of nucleosomal DNA at physiological ionic strength even in the absence of (H2A-H2B) dimer.


Subject(s)
DNA , Nucleic Acid Conformation , Nucleosomes , Histones , Models, Theoretical , Osmolar Concentration
13.
Mol Biol (Mosk) ; 21(3): 724-36, 1987.
Article in Russian | MEDLINE | ID: mdl-3657773

ABSTRACT

We have used the measurements of the histone fluorescence parameters to study the influence of the ionic strength on histone-DNA and histone-histone interactions in reconstructed nucleosomes. The ionic strength increase lead to the two-stage nucleosome dissociation. The dimer H2A-H2B dissociates at the first stage and the tetramer (H3-H4)2 at the second one. The dimer H2A-H2B dissociation from nucleosome is a two-stage process also. The ionic bonds between (H2A-H2B) histone dimer and DNA break at first and then the dissociation of dimer from histone tetramer (H3-H4)2 occurs. According to the proposed model the dissociation accompanying a nucleosome "swelling" and an increase of DNA curvature radius. It was shown that the energy of electrostatic interactions between histone dimer and DNA is sufficiently less than the energy of dimer-tetramer interaction. We propose that the nucleosome DNA ends interact with the dimer and tetramer simultaneously. The calculated number (approximately 30 divided by 40) of ionic bonds between DNA and histone octamer globular part practically coincides with the number of exposed cationic groups on the surface of octamer globular head. On this basis we have assumed that the spatial distribution of these groups is precisely determined, which explains the high evolutionary conservatism of the histone primary structure.


Subject(s)
DNA , Histones , Nucleosomes , Animals , Cattle , Macromolecular Substances , Spectrometry, Fluorescence , Thymus Gland
14.
Mol Biol (Mosk) ; 19(6): 1553-61, 1985.
Article in Russian | MEDLINE | ID: mdl-4079932

ABSTRACT

The salt-dependent structural changes of the histone octamer in complex with high-molecular-weight DNA have been studied by fluorescent spectroscopy. Changes in both the spectra maximum position and anisotropy of the histone tyrosine fluorescence reveal structural transitions in nucleosome within the ranges of 0.5-3 mM and 20-30 mM NaCl. Comparison of the octamer fluorescent parameters in complex with DNA as well as in a free state permits to interpret the revealed structural transitions as a change in degree of contacts stability between (H2A-H2B) dimer and (H3-H4)2 tetramer. More pronounced conformational changes in histone octamer are observed under the conditions of polynucleosome fibers interaction within the range of physiological ionic strength (100-600 mM NaCl). As far as fluorescent parameters are concerned, the aforementioned changes are connected with entire destruction of (H2A-H2B) dimer specific contacts with (H3-H4)2 tetramer. The obtained results suggest the possibility of existence of different structural states of histone octamer in the chromatin composition including those which are quite dissimilar from the octamer structure in the 2M NaCl solution.


Subject(s)
DNA/analysis , Histones/analysis , Nucleosomes/analysis , Animals , Cattle , In Vitro Techniques , Macromolecular Substances , Nucleic Acid Conformation , Osmolar Concentration , Protein Conformation , Spectrometry, Fluorescence , Thymus Gland/analysis , Urea
15.
Int J Biochem ; 17(2): 217-22, 1985.
Article in English | MEDLINE | ID: mdl-4007238

ABSTRACT

Tyr-72 is included in the hydrophobic cleft which is formed in the histone H1 globular head. Tyr-72 is screened against polar aqueous environment and its intramolecular mobility is sharply retarded. This microenvironment causes a red shift (lambda max = 279 nm) and a sharpening of the longer wavelength shoulder of absorption spectra, a high fluoresence anisotropy value (A = 0,11), high quantum yield of fluoresence (approximately 0.2) and a decrease of the Stern-Volmer Constant during quenching of histone H1 fluorescence by acrylamide. It has been found that the change in the intensity of histone fluorescence at lambda excit = 265 nm, but not at lambda excit = 280 nm, is due to the changes in the quantum yield of fluorescence. The increase of fluorescence intensity at lambda excit = 280 nm depends on the changes in the quantum yield and molar extinction coefficient of histone H1 tyrosyl chromophore. The change in the ratio of fluorescence intensity exited at 280 nm (F280) to the fluorescence intensity excited at 265 nm (F265) corresponds to the change of delta epsilon 286 in difference absorption spectra. The introduction of the parameter Cf = F280/F265 allows one to go over to studying excitation spectrum shifts instead of histone absorption spectrum shifts, which is much more convenient methodologically since in this case it is possible to carry out research using lower protein concentrations and turbid solutions. The results make it possible to designate Tyr-72 of histone H1 as a special class of fluorescent tyrosyls whose properties differ from those of tyrosyls of other tryptophane-free proteins: RNAase, insulin, core histones--H2A, H2B, H3, H4 and some others.


Subject(s)
Histones , Amino Acid Sequence , Animals , Cattle , Histones/isolation & purification , Macromolecular Substances , Osmolar Concentration , Protein Conformation , Sodium Chloride , Spectrometry, Fluorescence , Spectrophotometry, Ultraviolet , Thymus Gland
16.
Mol Biol (Mosk) ; 18(4): 979-87, 1984.
Article in Russian | MEDLINE | ID: mdl-6504036

ABSTRACT

By optical methods it has been previously shown that the globular "head" of histone H1 forms a hydrophobic cavity containing Tyr72. The latter is screened from the polar water surrounding and its intramolecular mobility is drastically hindered. As a consequence of the alteration in the micromilieu are a long wave shift (lambda max = 279,5 nm) and a more pronounced longwave absorption spectra, higher anisotropy (A = 0,11), augmented quantum yield of fluorescence (approximately 0,2) and a decrease of the Stern-Volmer constant for Hl at fluorescence quenching by acrylamide. It was found that changes in fluorescence intensity of histones are connected with alterations in the quantum yield of fluorescence at lambda exc = = 265 nm, but not at lambda exc = 280 nm. The changes in fluorescence intensity at light excitation 280 nm (F280) and 265 nm (F265) are in good accordance with shift delta E286 in differential absorption spectra. Introduction of parameter Cf = F280/F265 allows to study shifts of excitation spectra instead of shifts in absorption spectra of histones. This method has certain advantages, since it permits investigations with lower protein concentrations and in turbid solutions. The data obtained allow to draw out Tyr72 of histone Hl into a special class of fluorescent-tyrosyls, that differ in properties from those of other tryptophandevoided proteins: RNAse, insulin and core-histones H2A, H2B, H3 and H4.


Subject(s)
Histones , Animals , Cattle , Hydrogen-Ion Concentration , Protein Conformation , Spectrometry, Fluorescence , Thymus Gland/analysis
17.
Biofizika ; 28(4): 573-8, 1983.
Article in Russian | MEDLINE | ID: mdl-6615895

ABSTRACT

A model is proposed which describes the packing of polypeptide chains of histone molecules in the octamer (H3--H4--H2A--H2B)2, and interlocation of DNA and octamer in the nucleosome. DNA packing in the nucleosome is provided for by electrostatic interactions between DNA phosphates and cationic groups located on the globular part surface of histones octamer. The cationic groups of N- and C-end regions of the histone molecules (histones H3 and H4 in particular) additionally stabilize the nucleosome structure.


Subject(s)
DNA/metabolism , Histones/metabolism , Nucleosomes/ultrastructure , Animals , Cattle , Macromolecular Substances , Models, Molecular , Nucleic Acid Conformation , Nucleosomes/metabolism , Protein Conformation
18.
Biokhimiia ; 48(7): 1085-94, 1983 Jul.
Article in Russian | MEDLINE | ID: mdl-6615921

ABSTRACT

The amino acid composition of the H1-like histone isolated from carp spermatozoa (H1carp) is characterized by a high content of lysine (34.6%) and a low content of glycine (4.5%) as compared to that of its calf counterpart (H1calf). The Lys/Arg ratio is 21.6, which is much higher than that for the H1-like histones from other species spermatozoa (cf. echinodermata). It was shown that the fluorescence anisotropy and excitation spectra of histones H1carp and H1calf change synchronically. At the same time the final folding of the polypeptide chains of these histones within their ternary structure is different. These differences manifest themselves in a distinct quantum yield of both histones and different accessibility of the single tyrosine residue for fluorescence quenchers. In histone--DNA complexes the tyrosine fluorescence is quenched. An increase in the ionic strength gives rise to a formation of large-sized aggregates in a histone H1--DNA solution which contain structurally heterogenous histones H1 from different sources. Histone H1carp causes DNA aggregation at lower ionic strength values than its calf counterpart. The complexes are dissociated at 0.6 M NaCl.


Subject(s)
DNA/metabolism , Histones/metabolism , Spermatozoa/metabolism , Thymus Gland/metabolism , Amino Acids/analysis , Animals , Carps , Cattle , Fluorescence Polarization , Kinetics , Male , Nucleic Acid Conformation , Protein Conformation
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