Both autologous bone marrow mononuclear cell and peripheral blood progenitor cell therapies similarly improve ischaemia in patients with diabetic foot in comparison with control treatment.

BACKGROUND: The aim of our study was to compare the effect of bone marrow mononuclear cell and peripheral blood progenitor cell therapies in patients with diabetic foot disease and critical limb ischaemia unresponsive to revascularization with conservative therapy. METHODS: Twenty-eight patients with diabetic foot disease (17 treated by bone marrow cells and 11 by peripheral blood cell) were included into an active group and 22 patients into a control group without cell treatment. Transcutaneous oxygen pressure and rate of major amputation, as the main outcome measures, were compared between bone marrow cells, peripheral blood cell and control groups over 6 months; both cell therapy methods were also compared by the characteristics of cell suspensions. Possible adverse events were evaluated by changes of serum levels of angiogenic cytokines and retinal fundoscopic examination. RESULTS: The transcutaneous oxygen pressure increased significantly (p < 0.05) compared with baseline in both active groups after 6 months, with no significant differences between bone marrow cells and peripheral blood cell groups; however, no change of transcutaneous oxygen pressure in the control group was observed. The rate of major amputation by 6 months was significantly lower in the active cell therapy group compared with that in the control group (11.1% vs. 50%, p = 0.0032), with no difference between bone marrow cells and peripheral blood cell. A number of injected CD34+ cells and serum levels of angiogenic cytokines after treatment did not significantly differ between bone marrow cells and peripheral blood cell. CONCLUSIONS: Our study showed a superior benefit of bone marrow cells and peripheral blood cell treatments of critical limb ischaemia in patients with diabetic foot disease when compared with conservative therapy. There was no difference between both cell therapy groups, and no patient demonstrated signs of systemic vasculogenesis.

[Surgical treatment of gastrointestinal complications after the heart transplantation]. / Chirurgické výkony na gastrointestinálním traktu po transplantaci srdce.

BACKGROUND: Orthotopic heart transplantation (OHT) is standard treatment of patients with end-stage heart failure. Long-term immunosuppressive therapy leads to the increased risk of the infection and cancer. METHODS AND RESULTS: Our retrospective study was aimed to evaluate gastrointestinal surgery procedures after OHT. The retrospective study was carried out at the Institute of clinical and experimental medicine (IKEM), between January 1, 1996 and December 31, 2006. Our group of patients includes 587 transplant recipients and 22 gastrointestinal surgery procedures. CONCLUSIONS: Results from our department did not show statistical differences in the length of hospital stay, morbidity and mortality in comparison with patients after OHT and control group.

[Endoscopic transgastric drainage of a pancreatic pseudocyst--a cause of late perforation of the intestine]. / Endoskopická transgastrická drenáz pseudocysty pankreatu jako pricina pozdní perforace streva.

The authors present a case-review of a polymorbid patient, who underwent repetitive endoscopic transgastric drainage of the pancreatic pseudocyst in chronic pancreatitis. 3 months after the stents were released, the terminal ileus perforated. Ileocaecal resection was then completed. Further postoperative course was uncomplicated and the patient's current clinical condition is good.

[Laparoscopic rectopexis]. / Laparoskopická rektopexe.

INTRODUCTION: Rektopexis is an effective treatment method of rectal prolapses. Our retrospective study assessed the laparoscopic rectopexis results. METHODOLOGY: From 01-01-2003 to 31-11-2005, 10 patients were indicated for the procedure of laparoscopic retropexis. A "Vypro" mesh (Johnson&Johnson) was used to suspend the rectum. We fix it to the sacrum and to the rectum with an anchor (Eanchr, Johnson&Johnson). The follow-up examinations were conducted 2-30 months after the procedure. We assessed mortality rates, morbidity rates, the prolaps relapses incidence rates, obstipation incidence rates. RESULTS: The laparoscopic procedure was completed in 9 patients. In one case, we converted. The mortality and serious postoperative morbidity rate was 0%. The mean duration of hospitalization was 7 days. During the follow-up, no patient relapsed. One female patient complained of mild postoperative obstipation, which, however, did not require surgical revision. CONCLUSION: Laparoscopic rectopexis is a modern, safe and effective method of the prolaps of the rectum treatment.

[Planned laparoscopic procedures in clinical practice]. / Plánované laparoskopické výkony v klinické praxi.

Several recent randomized studies have proved that laparoscopic technique is feasible, safe and has been associated with better cosmetics, less pain, faster recovery, lower incidence of infectious complications, and shorter postoperative stay. However, these advantages are not substantial for patients with malignant diseases. New published randomized trials (laparoscopic gastrectomy, colectomy, nephrectomy) have proved that long-term survival data did not show any detrimental effect of laparoscopy in comparison with historic studies of open resections.

[Laparoscopic distal resection of the pancreas]. / Laparoskopická distální resekce pankreatu.

During the last two years, reports on laparoscopic procedures of the pancreas have been on increase. Laparoscopic resection of the pancreatic cauda is indicated, primarily, for benign cystic lesions of the cauda of the pancreas and for neuroendocrine tumors of the pancreas (mainly insulinomas). We have not recorded any report on the above procedure in the Czech literature. Therefore, in our case review, we have described laparoscopic distal resection of the pancreas with splenectomy for a pseudopapillary tumor of the pancreas.