ABSTRACT
A method was elaborated to construct combined artificial immunogens mimicking virus particles. The gist was exposing protein antigenic determinants of one virus on the particle surface and delivering plasmids with genes for antigenic proteins of another virus to specialized immune cells. Such immunogens were constructed and shown to induce biosynthesis of specific antibodies against HIV-1 and the tick-borne encephalitis virus. The level and duration of the humoral and cell responses were assayed.
Subject(s)
Drug Design , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunology , AIDS Vaccines/genetics , AIDS Vaccines/immunology , Animals , Antibodies/immunology , Antibodies/metabolism , Antibody Formation , Encephalitis Viruses, Tick-Borne/immunology , Epitopes/genetics , HIV Core Protein p24/genetics , HIV Core Protein p24/immunology , HIV-1/immunology , Mice , Mice, Inbred BALB C , Microscopy, Electron , Plasmids/genetics , Vaccines, Synthetic/pharmacology , Viral Proteins/chemistry , Viral Proteins/genetics , Viral Proteins/immunologySubject(s)
Genes, Synthetic , Models, Immunological , Recombinant Proteins , Vaccines, Combined/biosynthesis , Vaccines, Combined/immunology , Vaccines, Synthetic/biosynthesis , Vaccines, Synthetic/immunology , Animals , Antibodies/blood , Antibodies/immunology , Encephalitis Viruses, Tick-Borne/immunology , Escherichia coli , Immunoenzyme Techniques , Mice , Mice, Inbred BALB C , Proteins/genetics , Proteins/immunology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology , Th1 Cells/immunology , Th2 Cells/immunology , VaccinationABSTRACT
A method for designing molecular constructions of combined artificial immunogens mimicking viral particles is proposed. Using this method, it is possible to expose antigenic determinants of any viral protein on the surface of such particles and to deliver plasmids containing genes encoding the synthesis of protein antigens to target cells. This approach was used to create the constructions of combined artificial immunogens inducing the production of specific antibodies to HIV-1 and to evaluate the extent and duration of B-cellular immune response depending on the way of antigen exposure to immunocompetent cells.
Subject(s)
Vaccines, Combined/genetics , Vaccines, Synthetic/genetics , AIDS Vaccines/genetics , Animals , Epitopes/genetics , Gene Products, env/genetics , Gene Products, gag/genetics , HIV-1/genetics , HIV-1/immunology , Immunization , Mice , Mice, Inbred BALB C , Plasmids/genetics , Vaccines, Combined/immunology , Vaccines, Synthetic/immunologySubject(s)
Immunization/methods , Mycobacterium/chemistry , Mycobacterium/metabolism , Tuberculosis/prevention & control , Animals , B-Lymphocytes/immunology , Electrophoresis, Agar Gel , Interferon-gamma/metabolism , Mice , Mice, Inbred BALB C , Mycobacterium/immunology , Phagocytosis , T-Lymphocytes/immunology , Tumor Necrosis Factor-alpha/metabolismABSTRACT
The proposed method for assessing microorganism sensitivity in solid and semisolid liquid nutrient media provides more accurate results and permits isolation of resistant forms of bacteria and studies of antibiotic interactions. Bacteriological study of latent growth zones of bacteria, although rather long, makes the results more reliable. The method can be used for assessing the microflora sensitivity in patients without resorting to isolation of pure cultures and in biotechnology for isolation of new antibiotics.
