Essential and xenobiotic elements in cottage cheese from the Slovak market with a consumer risk assessment.

The examination of various elements in the milk products is very important in the food sector in respect of food quality and safety. The aim of this study was to determine the concentrations of calcium (Ca), cobalt (Co), cadmium (Cd), copper (Cu), chromium (Cr), iron (Fe), mercury (Hg), potassium (K), magnesium (Mg), sodium (Na), nickel (Ni), phosphorus (P), lead (Pb) and zinc (Zn) in white cottage cheese or cottage cheese supplemented with various additives (white, lacto-free, chive, tzatziki, mustard + onion, chili, active protein) available on the market of Slovakia. All essential elements were within the reference range. Cottage cheese enriched with tzatziki showed higher amount of Cu, Fe, K, and Zn. Mustard + onion cheese contained high values of Ca, Co, Mg, and Ni. In white cottage cheese high amount of Cr, Mn, and P was measured. The content of xenobiotic metals was below permitted limit. The contribution to PTWI (Provisional tolerable weekly intake) suggested very low dietary exposure to heavy metals as Cd, Hg, and Pb as well as other metals (Cu, Ni, and Zn) in cottage cheese. Numerous correlations between concentrations were observed. MOE (Margin of Exposure) evaluation denoted that average consumption of cottage cheese does not pose any high cardiovascular and nephrotoxicity threat.

Concentrations of selected trace elements in organs and tissues of livestock from a polluted area.

The occurrence of cadmium (Cd), nickel (Ni) and lead (Pb) from industrial emissions were determined in the muscle and liver of cattle from agricultural farms near an industrial plant in Eastern Slovakia. In this study cows (n = 25) were slaughtered and the concentrations of Cd, Ni, Pb were analyzed with a the atomic absorption spectrophotometer. Levels reaching the highest permissible hygiene limits for toxic metals Cd, Pb and Ni were determined in 50 samples. The maximum levels of Cd, Pb and Ni were recorded in the liver (0.865; 2.324; 1.140 mg/kg, respectively) and muscle (0.300; 0.854; 0.700 mg/kg, respectively). It was concluded that the exposure to an industrial plant significantly increases the levels of contaminants in the muscle and organs of cattle, as the most susceptible livestock.

The effects of long-term cadmium exposure in turkeys: accumulation and zinc prevention.

Experiments with turkeys were conducted to determine the effects of long-term ingestion of Cd (2.0 mg Cd/day/turkey) on its accumulation in the tissues and organs. The cadmium was found especially in the muscles, livers and kidneys. The highest average content of cadmium was found in the kidneys (1.09 mg/kg). The contents of Cd in the kidneys and livers were 19 times and 14 times (respectively) higher compared to the muscles. The administration of Zn (72 mg / day / turkey) along with high doses of Cd, significantly (P < 0.001) reduced the concentrations of Cd in the organs of the experimental animals. The average concentrations of cadmium in the kidneys and livers of turkeys from the CdZn group were 43 % and 48 % (respectively) lower than the average concentrations in the same organs in the turkeys from the group which received only Cd.

Content of selected metals in muscle of cyprinid fish species from the Nitra River, Slovakia.

OBJECTIVES: This study presents a rate of bioaccumulation of some metal concentrations in the muscle of five common cyprinid fish species (gudgeon - Gobio gobio, Europen chub - Leuciscus cephalus, barbel - Barbus barbus, roach - Rutilus rutilus, and nase - Chondrostoma nasus). DESIGN: Besides, correlations among selected metals as well as standard length and total weight and order of metal accumulation in the fish muscle were determined. RESULTS: Recorded metal concentrations (mg/kg wet weight basis) ranged as follows: Fe 4.23-22.95, Mn 0.10-0.72, Zn 16.26-166.90, Cu 15.39-25.97, Ni 0.00-3.72, Pb 0.00-1.81, and Cd 0.00-0.11. CONCLUSIONS: The Slovak permissible limit for Cu (10.0 mg/kg), Ni (0.5 mg/kg), Pb (0.2 mg/kg) and Cd (0.05 mg/kg) defined in the Codex Alimentarius for safe human consumption exceeded in 100%, 12%, 6% and 10% of analyzed samples for Cu, Ni, Pb and Cd respectively. On an average, the order of metal concentrations in the fish muscle was: Zn>Cu>Fe>Mn>Ni>Pb>Cd.

Effect of oral cadmium and zinc application on leukocytes biological activity in Japanese quails (Coturnix coturnix japonica).

Objective of this study was to investigate changes in biological activity of blood leukocytes in Japanese quails, caused by zinc and cadmium administration. Four groups of Japanese quails were used. Three experimental groups of quails were exposed either to Cd (0.12 mg Cd/quail), Zn (4 mg Zn/quail) or a combination of Cd and Zn (0.12 mg Cd, 4 mg Zn/quail), which were added daily to the drinking water. The fourth group was the control group. The metabolic activity of phagocytes and mitogenic activation of lymphocytes to phytohemaglutinine (PHA) were determined on day 37, 58 and 118 of exposure. The numbers of peripheral blood leukocytes of Japanese quail after cadmium and zinc addition in all groups of birds were without significant differences, however, the functional activities of phagocytes and lymphocytes in the Cd-group of quails were significantly decreased. The metabolic activity of phagocytes decreased significantly at all time points analysed (P < 0.05-0.001) in comparison to the control group and the Cd-Zn group. Similarly, the response of lymphocytes to PHA activation in the Cd exposed group of quails decreased significantly on day 58 and 118 of exposure when compared to the control group (P < 0.05) and Zn-group of quails (P < 0.01). Zn in combination with Cd eliminated the immunotoxic effect of Cd on metabolic activity of phagocytes and improved lymphocyte answer to PHA, when compared to the Cd-group on day 58 and 118 of metal administration. The results of the present study indicate that cadmium caused a significant decrease of metabolic activity of phagocytes and mitogenic activation of lymphocytes in peripheral blood of Japanese quails. Simultaneous administration of Zn and Cd eliminated the immunotoxic effect of Cd on functional activity of phagocytes and lymphocytes, and zinc improved both investigated functional parameters of immune cells.

The effect of cadmium in combination with zinc and selenium on ovarian structure in Japanese quails.

In this study the effect of cadmium, cadmium+selenium and cadmium+zinc administration on the ovarian structure in Japanese quails was studied. The morphometric analysis of the relative volume of primary follicles detected the highest value in control group with a similar value in the group with administration of cadmium with selenium. Lower relative volume is reported in group with cadmium and zinc administration and the group with simple cadmium administration (P < 0.05). The relative volume of growing follicles was very similar in all studied groups (11.33-15.35%), and the relative volume of stroma was very stable (82.59-86.45%). In the evaluation of the number of follicles undergoing atresia detected significantly higher number of atretic primary follicles as well as atretic growing follicles in the group with cadmium administration and cadmium with selenium administration in comparison with control group. In comparison of normal and atretic follicles we report the most negative effect of single cadmium administration on ovarian structure. Selenium co-administration shows protective effects but only the co-administration with zinc prevent significant cadmium ovarian alterations.

Seminal concentration of trace elements in fox and relationships to spermatozoa quality.

Concentrations of copper, zinc, iron, cadmium, lead, and nickel in the semen of foxes (Vulpes vulpes, n = 10), microscopic analysis of occurrence of pathological spermatozoa, and correlations of these elements with pathological forms were studied. Samples were analyzed by using an atomic absorption spectrophotometer. For analysis of pathological spermatozoa semen samples fixed with Hancock's solution and stained with Giemsa were prepared. For each fox at least 1000 spermatozoa were evaluated. The concentrations of copper, zinc, and iron in semen of foxes were found to be 2.16+/-0.53 mg/kg, 13.09+/-5.22 mg/kg, and 33.16+/-24.36 mg/kg, respectively, on wet weight basis. Concentration of cadmium was low (0.07+/-0.05 mg/kg). The levels of lead and nickel in the semen of foxes were 0.08+/-0.06 mg/kg and 0.35+/-0.24 mg/kg, respectively. The total percentage of pathological spermatozoa was 7.76+/-1.33% with predominancy of knob twisted flagellum, separated flagellum, and broken flagellum. In relation to trace elements the analysis showed significant (p < 0.05) correlation between copper and lead (r = -0.85), copper and other forms of pathological spermatozoa (r = -0.72), zinc and broken flagellum (r = -0.69), iron and retention of cytoplasmic drop (r = 0.87), cadmium and separated flagellum (r = -0.68), and between cadmium retention of cytoplasmic drop (r = 0.87).

Concentration of copper, iron, zinc, cadmium, lead, and nickel in boar semen and relation to the spermatozoa quality.

The concentration of copper, iron, zinc, cadmium, lead, and nickel as well as its relation to spermatozoa quality was investigated. The semen samples were analyzed by atomic absorption spectrophotometry (AAS). The concentration of copper in boar semen was 1.64 +/- 0.28 mg kg(-1) and of iron 16.14 +/- 10.35 mg kg(-1). The concentration of zinc in boar semen reached an average value of 171.74 +/- 64.72 mg kg(-1) and the level of cadmium reached 0.01-0.16 mg kg(-1) with the average value of 0.05 mg kg(-1). The analysis of lead showed that the concentration of this element in boar semen was 0.02 +/- 0.03 mg kg(-1) and the average level of nickel was 0.06 +/- 0.08 mg kg(-1). The total percentage of pathological spermatozoa was 9.82 +/- 1.47%. Detail analysis determined 3.18% of separated flagellum, 2.26% knob twisted flagellum, 0.88% flagellum torso, 0.85% flagellum ball, 0.42% broken flagellum, 0.23% retention of the cytoplasmic drop, 0.14% small heads, 0.03% large heads, and 1.83% forms other of pathological changes. Correlation analysis showed significant (p < 0.05) positive correlation between copper and lead (r = 0.52). High correlation between small head and knob twisted tail (r = 0.67), small head and broken flagellum (r = 0.88) as well as between small head and total number of pathological spermatozoa (r = 0.73) was determined.