In vitro and in vivo evaluation of the movement of Kepone in the rat submaxillary gland.

In vitro studies performed on slices of rat submaxillary gland showed that the rates of uptake and efflux of Kepone consisted of both a fast and a slow component. No significant alteration of these rates was observed after aeration with N2, decreased temperature, or incubations with cyanide or 2,4-dinitrophenol. In vivo data on actual concentration of Kepone in saliva and plasma over a 48 hour period showed no evidence of concentration of Kepone by the submaxillary glands. These data suggest that the movement of Kepone from blood into the saliva through the submaxillary glands occurs by means of passive diffusion.

The excretion of pesticides in saliva and its value in assessing exposure.

The possibility that pesticides are secreted in the saliva and that this secretion can be used to assess exposure to pesticides is discussed. Both Kepone and carbaryl were tested through a series of in vitro studies and in vivo experiments including both acute and subchronic studies. In the in vivo studies, both Kepone and carbaryl were detected in the saliva in the first twenty-four hours, but seldom thereafter. Analysis of urinary samples indicated a rapid clearance of the substances from the animals. In vitro uptake and efflux studies suggested that movement of both substances was by passive diffusion and reached a plateau after 60 minutes of incubation. Efflux rates were unaffected by experimental conditions, but were ten times faster for carbaryl.