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1.
Biodivers Data J ; 12: e114809, 2024.
Article in English | MEDLINE | ID: mdl-38283142

ABSTRACT

This paper describes a dataset of microbial communities from four different sponge species: Irciniaoros (Schmidt, 1864), Irciniavariabilis (Schmidt, 1862), Sarcotragusspinosulus Schmidt, 1862 and Sarcotragusfasciculatus (Pallas, 1766). The examined sponges all belong to Demospongiae (Class); Keratosa (Subclass); Dictyoceratida (Order); Irciniidae (Family). Samples were collected by scuba diving at depths between 6-14 m from two sampling sites of rocky formations at the northern coast of Crete (Cretan Sea, eastern Mediterranean) and were subjected to metabarcoding for the V5-V6 region of the 16S rRNA gene.

2.
Mol Phylogenet Evol ; 98: 147-60, 2016 May.
Article in English | MEDLINE | ID: mdl-26899923

ABSTRACT

Strains of Cyanobacteria isolated from mats of 9 thermal springs of Greece have been studied for their taxonomic evaluation. A polyphasic taxonomic approach was employed which included: morphological observations by light microscopy and scanning electron microscopy, maximum parsimony, maximum likelihood and Bayesian analysis of 16S rDNA sequences, secondary structural comparisons of 16S-23S rRNA Internal Transcribed Spacer sequences, and finally environmental data. The 17 cyanobacterial isolates formed a diverse group that contained filamentous, coccoid and heterocytous strains. These included representatives of the polyphyletic genera of Synechococcus and Phormidium, and the orders Oscillatoriales, Spirulinales, Chroococcales and Nostocales. After analysis, at least 6 new taxa at the genus level provide new evidence in the taxonomy of Cyanobacteria and highlight the abundant diversity of thermal spring environments with many potential endemic species or ecotypes.


Subject(s)
Cyanobacteria/classification , Hot Springs/microbiology , Bayes Theorem , Cyanobacteria/cytology , Cyanobacteria/genetics , Cyanobacteria/isolation & purification , Cyanobacteria/ultrastructure , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Greece , Likelihood Functions , Microscopy , Phylogeny , RNA, Bacterial/genetics , RNA, Ribosomal/genetics , Sequence Analysis, DNA
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